Cargando…

Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens

The intestinal microbiome is responsible for the fermentation of complex carbohydrates and orchestrates the immune system through gut microbiota-derived metabolites. In our previous study, we reported that supplementation of Enteromorpha polysaccharide (EP) and yeast glycoprotein (YG) in combination...

Descripción completa

Detalles Bibliográficos
Autores principales: Wassie, Teketay, Cheng, Bei, Zhou, Tiantian, Gao, Lumin, Lu, Zhuang, Xie, Chunyan, Wu, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614668/
https://www.ncbi.nlm.nih.gov/pubmed/36311785
http://dx.doi.org/10.3389/fimmu.2022.996897
_version_ 1784820247731109888
author Wassie, Teketay
Cheng, Bei
Zhou, Tiantian
Gao, Lumin
Lu, Zhuang
Xie, Chunyan
Wu, Xin
author_facet Wassie, Teketay
Cheng, Bei
Zhou, Tiantian
Gao, Lumin
Lu, Zhuang
Xie, Chunyan
Wu, Xin
author_sort Wassie, Teketay
collection PubMed
description The intestinal microbiome is responsible for the fermentation of complex carbohydrates and orchestrates the immune system through gut microbiota-derived metabolites. In our previous study, we reported that supplementation of Enteromorpha polysaccharide (EP) and yeast glycoprotein (YG) in combination synergistically improved antioxidant activities, serum lipid profile, and fatty acid metabolism in chicken. However, the mechanism of action of these polysaccharides remains elusive. The present study used an integrated 16S-rRNA sequencing technology and untargeted metabolomics technique to reveal the mechanism of action of EP+YG supplementation in broiler chickens fed basal diet or diets supplemented with EP+YG (200mg/kg EP + 200mg/kg YG). The results showed that EP+YG supplementation altered the overall structure of caecal microbiota as evidenced by β diversities analysis. Besides, EP+YG supplementation changed the microbiota composition by altering the community profile at the phylum and genus levels. Furthermore, Spearman correlation analysis indicated a significant correlation between altered microbiota genera vs serum cytokine levels and microbiota genera vs volatile fatty acids production. Predicted functional analysis showed that EP+YG supplementation significantly enriched amino acid metabolism, nucleotide metabolism, glycan biosynthesis and metabolism, energy metabolism, and carbohydrate metabolism. Metabolomics analysis confirmed that EP+YG supplementation modulates a myriad of caecal metabolites by increasing some metabolites, including pyruvic acid, pyridoxine, spermidine, spermine, and dopamine, and decreasing metabolites related to lipid metabolisms such as malonic acid, oleic acid, and docosahexaenoic acid. The quantitative enrichment analysis results further showed that glycolysis/gluconeogenesis, citric acid cycle, tyrosine metabolism, glycine, serine, and threonine metabolism, and cysteine and methionine metabolism were the most important enriched pathways identified with enrichment ratio >11, whereas, fatty acid biosynthesis and biosynthesis of unsaturated fatty acids pathways were suppressed. Together, the 16S-rRNA and untargeted metabolomics results uncovered that EP+YG supplementation modulates intestinal microbiota and their metabolites, thereby influencing the important metabolism pathways, suggesting a potential feed additive.
format Online
Article
Text
id pubmed-9614668
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96146682022-10-29 Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens Wassie, Teketay Cheng, Bei Zhou, Tiantian Gao, Lumin Lu, Zhuang Xie, Chunyan Wu, Xin Front Immunol Immunology The intestinal microbiome is responsible for the fermentation of complex carbohydrates and orchestrates the immune system through gut microbiota-derived metabolites. In our previous study, we reported that supplementation of Enteromorpha polysaccharide (EP) and yeast glycoprotein (YG) in combination synergistically improved antioxidant activities, serum lipid profile, and fatty acid metabolism in chicken. However, the mechanism of action of these polysaccharides remains elusive. The present study used an integrated 16S-rRNA sequencing technology and untargeted metabolomics technique to reveal the mechanism of action of EP+YG supplementation in broiler chickens fed basal diet or diets supplemented with EP+YG (200mg/kg EP + 200mg/kg YG). The results showed that EP+YG supplementation altered the overall structure of caecal microbiota as evidenced by β diversities analysis. Besides, EP+YG supplementation changed the microbiota composition by altering the community profile at the phylum and genus levels. Furthermore, Spearman correlation analysis indicated a significant correlation between altered microbiota genera vs serum cytokine levels and microbiota genera vs volatile fatty acids production. Predicted functional analysis showed that EP+YG supplementation significantly enriched amino acid metabolism, nucleotide metabolism, glycan biosynthesis and metabolism, energy metabolism, and carbohydrate metabolism. Metabolomics analysis confirmed that EP+YG supplementation modulates a myriad of caecal metabolites by increasing some metabolites, including pyruvic acid, pyridoxine, spermidine, spermine, and dopamine, and decreasing metabolites related to lipid metabolisms such as malonic acid, oleic acid, and docosahexaenoic acid. The quantitative enrichment analysis results further showed that glycolysis/gluconeogenesis, citric acid cycle, tyrosine metabolism, glycine, serine, and threonine metabolism, and cysteine and methionine metabolism were the most important enriched pathways identified with enrichment ratio >11, whereas, fatty acid biosynthesis and biosynthesis of unsaturated fatty acids pathways were suppressed. Together, the 16S-rRNA and untargeted metabolomics results uncovered that EP+YG supplementation modulates intestinal microbiota and their metabolites, thereby influencing the important metabolism pathways, suggesting a potential feed additive. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9614668/ /pubmed/36311785 http://dx.doi.org/10.3389/fimmu.2022.996897 Text en Copyright © 2022 Wassie, Cheng, Zhou, Gao, Lu, Xie and Wu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wassie, Teketay
Cheng, Bei
Zhou, Tiantian
Gao, Lumin
Lu, Zhuang
Xie, Chunyan
Wu, Xin
Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens
title Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens
title_full Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens
title_fullStr Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens
title_full_unstemmed Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens
title_short Microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary Enteromorpha polysaccharide and Yeast glycoprotein in chickens
title_sort microbiome-metabolome analysis reveals alterations in the composition and metabolism of caecal microbiota and metabolites with dietary enteromorpha polysaccharide and yeast glycoprotein in chickens
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614668/
https://www.ncbi.nlm.nih.gov/pubmed/36311785
http://dx.doi.org/10.3389/fimmu.2022.996897
work_keys_str_mv AT wassieteketay microbiomemetabolomeanalysisrevealsalterationsinthecompositionandmetabolismofcaecalmicrobiotaandmetaboliteswithdietaryenteromorphapolysaccharideandyeastglycoproteininchickens
AT chengbei microbiomemetabolomeanalysisrevealsalterationsinthecompositionandmetabolismofcaecalmicrobiotaandmetaboliteswithdietaryenteromorphapolysaccharideandyeastglycoproteininchickens
AT zhoutiantian microbiomemetabolomeanalysisrevealsalterationsinthecompositionandmetabolismofcaecalmicrobiotaandmetaboliteswithdietaryenteromorphapolysaccharideandyeastglycoproteininchickens
AT gaolumin microbiomemetabolomeanalysisrevealsalterationsinthecompositionandmetabolismofcaecalmicrobiotaandmetaboliteswithdietaryenteromorphapolysaccharideandyeastglycoproteininchickens
AT luzhuang microbiomemetabolomeanalysisrevealsalterationsinthecompositionandmetabolismofcaecalmicrobiotaandmetaboliteswithdietaryenteromorphapolysaccharideandyeastglycoproteininchickens
AT xiechunyan microbiomemetabolomeanalysisrevealsalterationsinthecompositionandmetabolismofcaecalmicrobiotaandmetaboliteswithdietaryenteromorphapolysaccharideandyeastglycoproteininchickens
AT wuxin microbiomemetabolomeanalysisrevealsalterationsinthecompositionandmetabolismofcaecalmicrobiotaandmetaboliteswithdietaryenteromorphapolysaccharideandyeastglycoproteininchickens