Cargando…

SARS-CoV-2 N95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating COVID-19

Background: SARS-CoV-2 N95 mask contamination in healthcare providers (HCPs) treating patients with COVID-19 is poorly understood. Method: We performed a prospective observational study of HCP N95 respirator SARS-CoV-2 contamination during aerosol-generating procedures (AGPs) on SARS-CoV-2–positive...

Descripción completa

Detalles Bibliográficos
Autores principales: Graves, Amanda, Warren, Bobby, Barrett, Aaron, Lewis, Sarah, Smith, Becky, Weber, David, Vavalle, Emily Sickbert-Bennett, Anderson, Deverick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cambridge University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614791/
http://dx.doi.org/10.1017/ash.2022.127
_version_ 1784820271038857216
author Graves, Amanda
Warren, Bobby
Barrett, Aaron
Lewis, Sarah
Smith, Becky
Weber, David
Vavalle, Emily Sickbert-Bennett
Anderson, Deverick
author_facet Graves, Amanda
Warren, Bobby
Barrett, Aaron
Lewis, Sarah
Smith, Becky
Weber, David
Vavalle, Emily Sickbert-Bennett
Anderson, Deverick
author_sort Graves, Amanda
collection PubMed
description Background: SARS-CoV-2 N95 mask contamination in healthcare providers (HCPs) treating patients with COVID-19 is poorly understood. Method: We performed a prospective observational study of HCP N95 respirator SARS-CoV-2 contamination during aerosol-generating procedures (AGPs) on SARS-CoV-2–positive patients housed in a COVID-19–specific unit at an academic medical center. Medical masks were used as surrogates for N95 respirators to avoid waste and were worn on top of HCP N95 respirators during study AGPs. Study masks were provided to HCPs while donning PPE and were retrieved during doffing. Additionally, during doffing, face shields were swabbed with Floq swabs premoistened with viral transport media (VTM) prior to disinfection. Medical masks were cut into 9 position-based pieces, placed in VTM, vortexed, and centrifuged (Fig. 1). RNA extraction and RT-PCR were completed on all samples. RT-PCR–positive samples underwent cell culture infection to detect cytopathic effects (CPE). Contamination was characterized by mask location and front and back of face shields. Patient COVID-19 symptoms were collected from routine clinical documentation. Study HCPs completed HCP-role–specific routine care (eg, assessing, administering medications, and maintaining oxygen supplementation) while in patient rooms and were observed by study team members. Results: We enrolled 31 HCPs between September and December 2021. HCP and patient characteristics are presented in Table 1. In total, 330 individual samples were obtained from 31 masks and 26 face shields among 12 patient rooms. Of the 330 samples, 0 samples were positive for SARS-CoV-2 via RT-PCR. Positive controls were successfully performed in the laboratory setting to confirm that the virus was recoverable using these methods. Notably, all samples were collected from HCPs caring for COVID-19 patients on high-flow, high-humidity Optiflow (AGP), with an average of 960 seconds (IQR, 525–1,680) spent in each room. In addition to Optiflow and routine care, study speech pathologists completed an additional AGP of fiberoptic endoscopic evaluation of swallowing. Notably, 29 (94%) of 31 study HCP had physical contact with their patient. Conclusions: Overall, mask contamination in HCPs treating patients with COVID-19 undergoing AGPs was not detectable while wearing face shields, despite patient contact and performing AGP. Funding: None Disclosures: None
format Online
Article
Text
id pubmed-9614791
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Cambridge University Press
record_format MEDLINE/PubMed
spelling pubmed-96147912022-10-29 SARS-CoV-2 N95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating COVID-19 Graves, Amanda Warren, Bobby Barrett, Aaron Lewis, Sarah Smith, Becky Weber, David Vavalle, Emily Sickbert-Bennett Anderson, Deverick Antimicrob Steward Healthc Epidemiol Covid-19 Background: SARS-CoV-2 N95 mask contamination in healthcare providers (HCPs) treating patients with COVID-19 is poorly understood. Method: We performed a prospective observational study of HCP N95 respirator SARS-CoV-2 contamination during aerosol-generating procedures (AGPs) on SARS-CoV-2–positive patients housed in a COVID-19–specific unit at an academic medical center. Medical masks were used as surrogates for N95 respirators to avoid waste and were worn on top of HCP N95 respirators during study AGPs. Study masks were provided to HCPs while donning PPE and were retrieved during doffing. Additionally, during doffing, face shields were swabbed with Floq swabs premoistened with viral transport media (VTM) prior to disinfection. Medical masks were cut into 9 position-based pieces, placed in VTM, vortexed, and centrifuged (Fig. 1). RNA extraction and RT-PCR were completed on all samples. RT-PCR–positive samples underwent cell culture infection to detect cytopathic effects (CPE). Contamination was characterized by mask location and front and back of face shields. Patient COVID-19 symptoms were collected from routine clinical documentation. Study HCPs completed HCP-role–specific routine care (eg, assessing, administering medications, and maintaining oxygen supplementation) while in patient rooms and were observed by study team members. Results: We enrolled 31 HCPs between September and December 2021. HCP and patient characteristics are presented in Table 1. In total, 330 individual samples were obtained from 31 masks and 26 face shields among 12 patient rooms. Of the 330 samples, 0 samples were positive for SARS-CoV-2 via RT-PCR. Positive controls were successfully performed in the laboratory setting to confirm that the virus was recoverable using these methods. Notably, all samples were collected from HCPs caring for COVID-19 patients on high-flow, high-humidity Optiflow (AGP), with an average of 960 seconds (IQR, 525–1,680) spent in each room. In addition to Optiflow and routine care, study speech pathologists completed an additional AGP of fiberoptic endoscopic evaluation of swallowing. Notably, 29 (94%) of 31 study HCP had physical contact with their patient. Conclusions: Overall, mask contamination in HCPs treating patients with COVID-19 undergoing AGPs was not detectable while wearing face shields, despite patient contact and performing AGP. Funding: None Disclosures: None Cambridge University Press 2022-05-16 /pmc/articles/PMC9614791/ http://dx.doi.org/10.1017/ash.2022.127 Text en © The Society for Healthcare Epidemiology of America 2022 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Covid-19
Graves, Amanda
Warren, Bobby
Barrett, Aaron
Lewis, Sarah
Smith, Becky
Weber, David
Vavalle, Emily Sickbert-Bennett
Anderson, Deverick
SARS-CoV-2 N95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating COVID-19
title SARS-CoV-2 N95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating COVID-19
title_full SARS-CoV-2 N95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating COVID-19
title_fullStr SARS-CoV-2 N95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating COVID-19
title_full_unstemmed SARS-CoV-2 N95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating COVID-19
title_short SARS-CoV-2 N95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating COVID-19
title_sort sars-cov-2 n95 contamination worn under a face shield, via medical mask surrogate, in healthcare providers treating covid-19
topic Covid-19
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9614791/
http://dx.doi.org/10.1017/ash.2022.127
work_keys_str_mv AT gravesamanda sarscov2n95contaminationwornunderafaceshieldviamedicalmasksurrogateinhealthcareproviderstreatingcovid19
AT warrenbobby sarscov2n95contaminationwornunderafaceshieldviamedicalmasksurrogateinhealthcareproviderstreatingcovid19
AT barrettaaron sarscov2n95contaminationwornunderafaceshieldviamedicalmasksurrogateinhealthcareproviderstreatingcovid19
AT lewissarah sarscov2n95contaminationwornunderafaceshieldviamedicalmasksurrogateinhealthcareproviderstreatingcovid19
AT smithbecky sarscov2n95contaminationwornunderafaceshieldviamedicalmasksurrogateinhealthcareproviderstreatingcovid19
AT weberdavid sarscov2n95contaminationwornunderafaceshieldviamedicalmasksurrogateinhealthcareproviderstreatingcovid19
AT vavalleemilysickbertbennett sarscov2n95contaminationwornunderafaceshieldviamedicalmasksurrogateinhealthcareproviderstreatingcovid19
AT andersondeverick sarscov2n95contaminationwornunderafaceshieldviamedicalmasksurrogateinhealthcareproviderstreatingcovid19