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The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants
Plants and algae are faced with a conundrum: harvesting sufficient light to drive their metabolic needs while dissipating light in excess to prevent photodamage, a process known as nonphotochemical quenching. A slowly relaxing form of energy dissipation, termed qH, is critical for plants’ survival u...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615032/ https://www.ncbi.nlm.nih.gov/pubmed/36152752 http://dx.doi.org/10.1016/j.jbc.2022.102519 |
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author | Bru, Pierrick Steen, Collin J. Park, Soomin Amstutz, Cynthia L. Sylak-Glassman, Emily J. Lam, Lam Fekete, Agnes Mueller, Martin J. Longoni, Fiamma Fleming, Graham R. Niyogi, Krishna K. Malnoë, Alizée |
author_facet | Bru, Pierrick Steen, Collin J. Park, Soomin Amstutz, Cynthia L. Sylak-Glassman, Emily J. Lam, Lam Fekete, Agnes Mueller, Martin J. Longoni, Fiamma Fleming, Graham R. Niyogi, Krishna K. Malnoë, Alizée |
author_sort | Bru, Pierrick |
collection | PubMed |
description | Plants and algae are faced with a conundrum: harvesting sufficient light to drive their metabolic needs while dissipating light in excess to prevent photodamage, a process known as nonphotochemical quenching. A slowly relaxing form of energy dissipation, termed qH, is critical for plants’ survival under abiotic stress; however, qH location in the photosynthetic membrane is unresolved. Here, we tested whether we could isolate subcomplexes from plants in which qH was induced that would remain in an energy-dissipative state. Interestingly, we found that chlorophyll (Chl) fluorescence lifetimes were decreased by qH in isolated major trimeric antenna complexes, indicating that they serve as a site for qH-energy dissipation and providing a natively quenched complex with physiological relevance to natural conditions. Next, we monitored the changes in thylakoid pigment, protein, and lipid contents of antenna with active or inactive qH but did not detect any evident differences. Finally, we investigated whether specific subunits of the major antenna complexes were required for qH but found that qH was insensitive to trimer composition. Because we previously observed that qH can occur in the absence of specific xanthophylls, and no evident changes in pigments, proteins, or lipids were detected, we tentatively propose that the energy-dissipative state reported here may stem from Chl–Chl excitonic interaction. |
format | Online Article Text |
id | pubmed-9615032 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-96150322022-10-31 The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants Bru, Pierrick Steen, Collin J. Park, Soomin Amstutz, Cynthia L. Sylak-Glassman, Emily J. Lam, Lam Fekete, Agnes Mueller, Martin J. Longoni, Fiamma Fleming, Graham R. Niyogi, Krishna K. Malnoë, Alizée J Biol Chem Research Article Plants and algae are faced with a conundrum: harvesting sufficient light to drive their metabolic needs while dissipating light in excess to prevent photodamage, a process known as nonphotochemical quenching. A slowly relaxing form of energy dissipation, termed qH, is critical for plants’ survival under abiotic stress; however, qH location in the photosynthetic membrane is unresolved. Here, we tested whether we could isolate subcomplexes from plants in which qH was induced that would remain in an energy-dissipative state. Interestingly, we found that chlorophyll (Chl) fluorescence lifetimes were decreased by qH in isolated major trimeric antenna complexes, indicating that they serve as a site for qH-energy dissipation and providing a natively quenched complex with physiological relevance to natural conditions. Next, we monitored the changes in thylakoid pigment, protein, and lipid contents of antenna with active or inactive qH but did not detect any evident differences. Finally, we investigated whether specific subunits of the major antenna complexes were required for qH but found that qH was insensitive to trimer composition. Because we previously observed that qH can occur in the absence of specific xanthophylls, and no evident changes in pigments, proteins, or lipids were detected, we tentatively propose that the energy-dissipative state reported here may stem from Chl–Chl excitonic interaction. American Society for Biochemistry and Molecular Biology 2022-09-22 /pmc/articles/PMC9615032/ /pubmed/36152752 http://dx.doi.org/10.1016/j.jbc.2022.102519 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Bru, Pierrick Steen, Collin J. Park, Soomin Amstutz, Cynthia L. Sylak-Glassman, Emily J. Lam, Lam Fekete, Agnes Mueller, Martin J. Longoni, Fiamma Fleming, Graham R. Niyogi, Krishna K. Malnoë, Alizée The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants |
title | The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants |
title_full | The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants |
title_fullStr | The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants |
title_full_unstemmed | The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants |
title_short | The major trimeric antenna complexes serve as a site for qH-energy dissipation in plants |
title_sort | major trimeric antenna complexes serve as a site for qh-energy dissipation in plants |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615032/ https://www.ncbi.nlm.nih.gov/pubmed/36152752 http://dx.doi.org/10.1016/j.jbc.2022.102519 |
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