Cargando…
The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation
The heme enzyme myeloperoxidase (MPO) is one of the key players in the neutrophil-mediated killing of invading pathogens as part of the innate immune system. MPO generates antimicrobial oxidants, which indiscriminately and effectively kill phagocytosed pathogens. Staphylococcus aureus, however, is a...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Biochemistry and Molecular Biology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615034/ https://www.ncbi.nlm.nih.gov/pubmed/36150500 http://dx.doi.org/10.1016/j.jbc.2022.102514 |
_version_ | 1784820329508503552 |
---|---|
author | Leitgeb, Urban Furtmüller, Paul G. Hofbauer, Stefan Brito, Jose A. Obinger, Christian Pfanzagl, Vera |
author_facet | Leitgeb, Urban Furtmüller, Paul G. Hofbauer, Stefan Brito, Jose A. Obinger, Christian Pfanzagl, Vera |
author_sort | Leitgeb, Urban |
collection | PubMed |
description | The heme enzyme myeloperoxidase (MPO) is one of the key players in the neutrophil-mediated killing of invading pathogens as part of the innate immune system. MPO generates antimicrobial oxidants, which indiscriminately and effectively kill phagocytosed pathogens. Staphylococcus aureus, however, is able to escape this fate, in part by secreting a small protein called SPIN (Staphylococcal Peroxidase Inhibitor), which specifically targets and inhibits MPO in a structurally complex manner. Here, we present the first crystal structures of the complex of SPIN-aureus and a truncated version (SPIN-truncated) with mature dimeric leukocyte MPO. We unravel the contributions of the two domains to the kinetics and thermodynamics of SPIN-aureus binding to MPO by using a broad array of complementary biochemical and biophysical methods. The C-terminal “recognition” domain is shown to mediate specific binding to MPO, while interaction of the N-terminal “inhibitory” domain is guided mainly by hydrophobic effects and thus is less sequence dependent. We found that inhibition of MPO is achieved by reducing substrate migration, but SPIN-aureus cannot completely block MPO activity. Its’ effectiveness is inversely related to substrate size, with no discernible dependence on other factors. Thus, SPIN-aureus is an extremely high-affinity inhibitor and highly efficient for substrates larger than halogens. As aberrant MPO activity is implicated in a number of chronic inflammatory diseases, SPIN-aureus is the first promising protein inhibitor for specific inhibition of human MPO. |
format | Online Article Text |
id | pubmed-9615034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-96150342022-10-31 The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation Leitgeb, Urban Furtmüller, Paul G. Hofbauer, Stefan Brito, Jose A. Obinger, Christian Pfanzagl, Vera J Biol Chem Research Article The heme enzyme myeloperoxidase (MPO) is one of the key players in the neutrophil-mediated killing of invading pathogens as part of the innate immune system. MPO generates antimicrobial oxidants, which indiscriminately and effectively kill phagocytosed pathogens. Staphylococcus aureus, however, is able to escape this fate, in part by secreting a small protein called SPIN (Staphylococcal Peroxidase Inhibitor), which specifically targets and inhibits MPO in a structurally complex manner. Here, we present the first crystal structures of the complex of SPIN-aureus and a truncated version (SPIN-truncated) with mature dimeric leukocyte MPO. We unravel the contributions of the two domains to the kinetics and thermodynamics of SPIN-aureus binding to MPO by using a broad array of complementary biochemical and biophysical methods. The C-terminal “recognition” domain is shown to mediate specific binding to MPO, while interaction of the N-terminal “inhibitory” domain is guided mainly by hydrophobic effects and thus is less sequence dependent. We found that inhibition of MPO is achieved by reducing substrate migration, but SPIN-aureus cannot completely block MPO activity. Its’ effectiveness is inversely related to substrate size, with no discernible dependence on other factors. Thus, SPIN-aureus is an extremely high-affinity inhibitor and highly efficient for substrates larger than halogens. As aberrant MPO activity is implicated in a number of chronic inflammatory diseases, SPIN-aureus is the first promising protein inhibitor for specific inhibition of human MPO. American Society for Biochemistry and Molecular Biology 2022-09-21 /pmc/articles/PMC9615034/ /pubmed/36150500 http://dx.doi.org/10.1016/j.jbc.2022.102514 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Leitgeb, Urban Furtmüller, Paul G. Hofbauer, Stefan Brito, Jose A. Obinger, Christian Pfanzagl, Vera The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation |
title | The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation |
title_full | The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation |
title_fullStr | The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation |
title_full_unstemmed | The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation |
title_short | The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation |
title_sort | staphylococcal inhibitory protein spin binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615034/ https://www.ncbi.nlm.nih.gov/pubmed/36150500 http://dx.doi.org/10.1016/j.jbc.2022.102514 |
work_keys_str_mv | AT leitgeburban thestaphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT furtmullerpaulg thestaphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT hofbauerstefan thestaphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT britojosea thestaphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT obingerchristian thestaphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT pfanzaglvera thestaphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT leitgeburban staphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT furtmullerpaulg staphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT hofbauerstefan staphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT britojosea staphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT obingerchristian staphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation AT pfanzaglvera staphylococcalinhibitoryproteinspinbindstohumanmyeloperoxidasewithpicomolaraffinitybutonlydampenshalideoxidation |