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scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells

Single-cell DNA methylation profiling currently suffers from excessive noise and/or limited cellular throughput. We developed scTAM-seq, a targeted bisulfite-free method for profiling up to 650 CpGs in up to 10,000 cells per experiment, with a dropout rate as low as 7%. We demonstrate that scTAM-seq...

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Detalles Bibliográficos
Autores principales:	Bianchi, Agostina, Scherer, Michael, Zaurin, Roser, Quililan, Kimberly, Velten, Lars, Beekman, Renée
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615163/ https://www.ncbi.nlm.nih.gov/pubmed/36307828 http://dx.doi.org/10.1186/s13059-022-02796-7

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