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Ttc39c is a potential target for the treatment of lung cancer

BACKGROUND: The novel TTC gene, tetratricopeptide repeat domain 39 C (Ttc39c), mainly mediates the interaction between proteins. It is involved in the progression of various tumors. In this study, we determined the effect of Ttc39c on lung adenocarcinoma and found that it might be used as a potentia...

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Autores principales: Rong, Hao, Peng, Jun, Ma, Ke, Zhu, Jiang, He, Jin-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615393/
https://www.ncbi.nlm.nih.gov/pubmed/36303158
http://dx.doi.org/10.1186/s12890-022-02173-x
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author Rong, Hao
Peng, Jun
Ma, Ke
Zhu, Jiang
He, Jin-Tao
author_facet Rong, Hao
Peng, Jun
Ma, Ke
Zhu, Jiang
He, Jin-Tao
author_sort Rong, Hao
collection PubMed
description BACKGROUND: The novel TTC gene, tetratricopeptide repeat domain 39 C (Ttc39c), mainly mediates the interaction between proteins. It is involved in the progression of various tumors. In this study, we determined the effect of Ttc39c on lung adenocarcinoma and found that it might be used as a potential intervention target. METHODS: We performed a difference analysis of Ttc39c samples from the TCGA database. Transwell experiments were conducted to determine the ability of cell metastasis. Celigo and MTT assays were performed to determine the effect of Ttc39c gene subtraction on cell proliferation. FACS was performed to determine the effect of Ttc39c gene subtraction on apoptosis. Clone-formation experiments were conducted to determine the effect of Ttc39c gene subtraction on cloning ability. Transcriptomics, proteomics, and metabolomics were used to elucidate the enrichment pathway of the Ttc39c gene in the progression of lung adenocarcinoma. RESULTS: The expression of Ttc39c increased significantly in lung adenocarcinoma. The proliferation, metastasis, and cloning ability of human lung cancer cells were inhibited, while the apoptosis of cells increased significantly after the depletion of Ttc39c. Our results based on the transcriptomics, proteomics, and metabolomics analyses indicated that Ttc39c might be involved in the progression of lung adenocarcinoma (LUAD) mainly through the metabolic pathway and the p53 pathway. CONCLUSION: To summarize, Ttc39c strongly regulates the proliferation and metastasis of lung adenocarcinoma cells. The main pathways involved in Ttc39c in lung adenocarcinoma include the energy metabolism and p53 pathways. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12890-022-02173-x.
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spelling pubmed-96153932022-10-29 Ttc39c is a potential target for the treatment of lung cancer Rong, Hao Peng, Jun Ma, Ke Zhu, Jiang He, Jin-Tao BMC Pulm Med Research BACKGROUND: The novel TTC gene, tetratricopeptide repeat domain 39 C (Ttc39c), mainly mediates the interaction between proteins. It is involved in the progression of various tumors. In this study, we determined the effect of Ttc39c on lung adenocarcinoma and found that it might be used as a potential intervention target. METHODS: We performed a difference analysis of Ttc39c samples from the TCGA database. Transwell experiments were conducted to determine the ability of cell metastasis. Celigo and MTT assays were performed to determine the effect of Ttc39c gene subtraction on cell proliferation. FACS was performed to determine the effect of Ttc39c gene subtraction on apoptosis. Clone-formation experiments were conducted to determine the effect of Ttc39c gene subtraction on cloning ability. Transcriptomics, proteomics, and metabolomics were used to elucidate the enrichment pathway of the Ttc39c gene in the progression of lung adenocarcinoma. RESULTS: The expression of Ttc39c increased significantly in lung adenocarcinoma. The proliferation, metastasis, and cloning ability of human lung cancer cells were inhibited, while the apoptosis of cells increased significantly after the depletion of Ttc39c. Our results based on the transcriptomics, proteomics, and metabolomics analyses indicated that Ttc39c might be involved in the progression of lung adenocarcinoma (LUAD) mainly through the metabolic pathway and the p53 pathway. CONCLUSION: To summarize, Ttc39c strongly regulates the proliferation and metastasis of lung adenocarcinoma cells. The main pathways involved in Ttc39c in lung adenocarcinoma include the energy metabolism and p53 pathways. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12890-022-02173-x. BioMed Central 2022-10-27 /pmc/articles/PMC9615393/ /pubmed/36303158 http://dx.doi.org/10.1186/s12890-022-02173-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Rong, Hao
Peng, Jun
Ma, Ke
Zhu, Jiang
He, Jin-Tao
Ttc39c is a potential target for the treatment of lung cancer
title Ttc39c is a potential target for the treatment of lung cancer
title_full Ttc39c is a potential target for the treatment of lung cancer
title_fullStr Ttc39c is a potential target for the treatment of lung cancer
title_full_unstemmed Ttc39c is a potential target for the treatment of lung cancer
title_short Ttc39c is a potential target for the treatment of lung cancer
title_sort ttc39c is a potential target for the treatment of lung cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615393/
https://www.ncbi.nlm.nih.gov/pubmed/36303158
http://dx.doi.org/10.1186/s12890-022-02173-x
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