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Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices

During non-rapid eye movement (NREM) sleep, cortical neuron activity alternates between a depolarized (firing, up-state) and a hyperpolarized state (down-state) coinciding with delta electroencephalogram (EEG) slow-wave oscillation (SWO, 0. 5–4 Hz) in vivo. Recently, we have found that artificial sl...

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Autores principales: Besing, Gai-Linn Kay, St. John, Emily Kate, Potesta, Cobie Victoria, Gallagher, Martin J., Zhou, Chengwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615418/
https://www.ncbi.nlm.nih.gov/pubmed/36313618
http://dx.doi.org/10.3389/fncel.2022.948327
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author Besing, Gai-Linn Kay
St. John, Emily Kate
Potesta, Cobie Victoria
Gallagher, Martin J.
Zhou, Chengwen
author_facet Besing, Gai-Linn Kay
St. John, Emily Kate
Potesta, Cobie Victoria
Gallagher, Martin J.
Zhou, Chengwen
author_sort Besing, Gai-Linn Kay
collection PubMed
description During non-rapid eye movement (NREM) sleep, cortical neuron activity alternates between a depolarized (firing, up-state) and a hyperpolarized state (down-state) coinciding with delta electroencephalogram (EEG) slow-wave oscillation (SWO, 0. 5–4 Hz) in vivo. Recently, we have found that artificial sleep-like up/down-states can potentiate synaptic strength in layer V cortical neurons ex vivo. Using mouse coronal brain slices, whole cell voltage-clamp recordings were made from layer V cortical pyramidal neurons to record spontaneous excitatory synaptic currents (sEPSCs) and inhibitory synaptic currents (sIPSCs). Artificial sleep-like up/down-states (as SWOs, 0.5 Hz, 10 min, current clamp mode) were induced by injecting sinusoidal currents into layer V cortical neurons. Baseline pre-SWO recordings were recorded for 5 min and post-SWO recordings for at least 25–30 min. Compared to pre-SWO sEPSCs or sIPSCs, post-SWO sEPSCs or sIPSCs in layer V cortical neurons exhibited significantly larger amplitudes and a higher frequency for 30 min. This finding suggests that both sEPSCs and sIPSCs could be potentiated in layer V cortical neurons by the low-level activity of SWOs, and sEPSCs and sIPSCs maintained a balance in layer V cortical neurons during pre- and post-SWO periods. Overall, this study presents an ex vivo method to show SWO's ability to induce synaptic plasticity in layer V cortical neurons, which may underlie sleep-related synaptic potentiation for sleep-related memory consolidation in vivo.
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spelling pubmed-96154182022-10-29 Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices Besing, Gai-Linn Kay St. John, Emily Kate Potesta, Cobie Victoria Gallagher, Martin J. Zhou, Chengwen Front Cell Neurosci Cellular Neuroscience During non-rapid eye movement (NREM) sleep, cortical neuron activity alternates between a depolarized (firing, up-state) and a hyperpolarized state (down-state) coinciding with delta electroencephalogram (EEG) slow-wave oscillation (SWO, 0. 5–4 Hz) in vivo. Recently, we have found that artificial sleep-like up/down-states can potentiate synaptic strength in layer V cortical neurons ex vivo. Using mouse coronal brain slices, whole cell voltage-clamp recordings were made from layer V cortical pyramidal neurons to record spontaneous excitatory synaptic currents (sEPSCs) and inhibitory synaptic currents (sIPSCs). Artificial sleep-like up/down-states (as SWOs, 0.5 Hz, 10 min, current clamp mode) were induced by injecting sinusoidal currents into layer V cortical neurons. Baseline pre-SWO recordings were recorded for 5 min and post-SWO recordings for at least 25–30 min. Compared to pre-SWO sEPSCs or sIPSCs, post-SWO sEPSCs or sIPSCs in layer V cortical neurons exhibited significantly larger amplitudes and a higher frequency for 30 min. This finding suggests that both sEPSCs and sIPSCs could be potentiated in layer V cortical neurons by the low-level activity of SWOs, and sEPSCs and sIPSCs maintained a balance in layer V cortical neurons during pre- and post-SWO periods. Overall, this study presents an ex vivo method to show SWO's ability to induce synaptic plasticity in layer V cortical neurons, which may underlie sleep-related synaptic potentiation for sleep-related memory consolidation in vivo. Frontiers Media S.A. 2022-10-14 /pmc/articles/PMC9615418/ /pubmed/36313618 http://dx.doi.org/10.3389/fncel.2022.948327 Text en Copyright © 2022 Besing, St. John, Potesta, Gallagher and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Besing, Gai-Linn Kay
St. John, Emily Kate
Potesta, Cobie Victoria
Gallagher, Martin J.
Zhou, Chengwen
Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices
title Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices
title_full Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices
title_fullStr Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices
title_full_unstemmed Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices
title_short Artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices
title_sort artificial sleep-like up/down-states induce synaptic plasticity in cortical neurons from mouse brain slices
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615418/
https://www.ncbi.nlm.nih.gov/pubmed/36313618
http://dx.doi.org/10.3389/fncel.2022.948327
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