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The two-component system CpxA/CpxR is critical for full virulence in Actinobacillus pleuropneumoniae
Actinobacillus pleuropneumoniae, a major bacterial porcine respiratory tract pathogen causing pig pleuropneumonia, has resulted in high economic losses worldwide. The mutation of the two-component system CpxAR strongly impacted the virulence of A. pleuropneumoniae, but the underlying regulatory mech...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615922/ https://www.ncbi.nlm.nih.gov/pubmed/36312949 http://dx.doi.org/10.3389/fmicb.2022.1029426 |
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author | Liu, Feng Yao, Qing Huang, Jing Wan, Jiajia Xie, Tingting Gao, Xuejun Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng |
author_facet | Liu, Feng Yao, Qing Huang, Jing Wan, Jiajia Xie, Tingting Gao, Xuejun Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng |
author_sort | Liu, Feng |
collection | PubMed |
description | Actinobacillus pleuropneumoniae, a major bacterial porcine respiratory tract pathogen causing pig pleuropneumonia, has resulted in high economic losses worldwide. The mutation of the two-component system CpxAR strongly impacted the virulence of A. pleuropneumoniae, but the underlying regulatory mechanism remained unclear. Here, we found that CpxAR positively regulated the cpxDCBA gene cluster involved in polysaccharide capsule export. A capsular layer was confirmed in wild-type cells by transmission electron microscopy, whereas cpxAR and cpxD mutants were non-capsulated. The mutants for polysaccharide capsule export gene cpxD exhibited non-capsulated and were strongly impaired in virulence for mice, indicating a major role of CPS export system in virulence. We then demonstrated that CpxR directly regulated the transcription of the CPS export gene cluster cpxDCBA. Taken together, our data suggested that CpxAR is a key modulator of capsule export that facilitates A. pleuropneumoniae survival in the host. |
format | Online Article Text |
id | pubmed-9615922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96159222022-10-29 The two-component system CpxA/CpxR is critical for full virulence in Actinobacillus pleuropneumoniae Liu, Feng Yao, Qing Huang, Jing Wan, Jiajia Xie, Tingting Gao, Xuejun Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng Front Microbiol Microbiology Actinobacillus pleuropneumoniae, a major bacterial porcine respiratory tract pathogen causing pig pleuropneumonia, has resulted in high economic losses worldwide. The mutation of the two-component system CpxAR strongly impacted the virulence of A. pleuropneumoniae, but the underlying regulatory mechanism remained unclear. Here, we found that CpxAR positively regulated the cpxDCBA gene cluster involved in polysaccharide capsule export. A capsular layer was confirmed in wild-type cells by transmission electron microscopy, whereas cpxAR and cpxD mutants were non-capsulated. The mutants for polysaccharide capsule export gene cpxD exhibited non-capsulated and were strongly impaired in virulence for mice, indicating a major role of CPS export system in virulence. We then demonstrated that CpxR directly regulated the transcription of the CPS export gene cluster cpxDCBA. Taken together, our data suggested that CpxAR is a key modulator of capsule export that facilitates A. pleuropneumoniae survival in the host. Frontiers Media S.A. 2022-10-05 /pmc/articles/PMC9615922/ /pubmed/36312949 http://dx.doi.org/10.3389/fmicb.2022.1029426 Text en Copyright © 2022 Liu, Yao, Huang, Wan, Xie, Gao, Sun, Zhang, Bei and Lei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Liu, Feng Yao, Qing Huang, Jing Wan, Jiajia Xie, Tingting Gao, Xuejun Sun, Diangang Zhang, Fuxian Bei, Weicheng Lei, Liancheng The two-component system CpxA/CpxR is critical for full virulence in Actinobacillus pleuropneumoniae |
title | The two-component system CpxA/CpxR is critical for full virulence in Actinobacillus pleuropneumoniae |
title_full | The two-component system CpxA/CpxR is critical for full virulence in Actinobacillus pleuropneumoniae |
title_fullStr | The two-component system CpxA/CpxR is critical for full virulence in Actinobacillus pleuropneumoniae |
title_full_unstemmed | The two-component system CpxA/CpxR is critical for full virulence in Actinobacillus pleuropneumoniae |
title_short | The two-component system CpxA/CpxR is critical for full virulence in Actinobacillus pleuropneumoniae |
title_sort | two-component system cpxa/cpxr is critical for full virulence in actinobacillus pleuropneumoniae |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9615922/ https://www.ncbi.nlm.nih.gov/pubmed/36312949 http://dx.doi.org/10.3389/fmicb.2022.1029426 |
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