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High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616521/ https://www.ncbi.nlm.nih.gov/pubmed/36036114 http://dx.doi.org/10.1111/pbi.13915 |
| _version_ | 1784820660565966848 |
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| author | Chen, Zhenfan Zhu, Jiayi Chen, Zixi Du, Ming Yao, Rao Fu, Wen Lei, Anping Wang, Jiangxin |
| author_facet | Chen, Zhenfan Zhu, Jiayi Chen, Zixi Du, Ming Yao, Rao Fu, Wen Lei, Anping Wang, Jiangxin |
| author_sort | Chen, Zhenfan |
| collection | PubMed |
| description | |
| format | Online Article Text |
| id | pubmed-9616521 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-96165212022-10-31 High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis Chen, Zhenfan Zhu, Jiayi Chen, Zixi Du, Ming Yao, Rao Fu, Wen Lei, Anping Wang, Jiangxin Plant Biotechnol J Brief Communications John Wiley and Sons Inc. 2022-09-15 2022-11 /pmc/articles/PMC9616521/ /pubmed/36036114 http://dx.doi.org/10.1111/pbi.13915 Text en © 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
| spellingShingle | Brief Communications Chen, Zhenfan Zhu, Jiayi Chen, Zixi Du, Ming Yao, Rao Fu, Wen Lei, Anping Wang, Jiangxin High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis |
| title | High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis
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| title_full | High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis
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| title_fullStr | High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis
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| title_full_unstemmed | High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis
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| title_short | High‐throughput sequencing revealed low‐efficacy genome editing using Cas9 RNPs electroporation and single‐celled microinjection provided an alternative to deliver CRISPR reagents into Euglena gracilis
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| title_sort | high‐throughput sequencing revealed low‐efficacy genome editing using cas9 rnps electroporation and single‐celled microinjection provided an alternative to deliver crispr reagents into euglena gracilis |
| topic | Brief Communications |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616521/ https://www.ncbi.nlm.nih.gov/pubmed/36036114 http://dx.doi.org/10.1111/pbi.13915 |
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