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Impact of One-Week Administration of Dihydrotestosterone in Rat Anterior Pituitary Gland

Hyperandrogenism causes dysfunction of the hypothalamic–pituitary–gonadal (HPG) axis in reproductive women. In this study, we examined the effects of dihydrotestosterone (DHT) on characteristic changes in rat anterior pituitary gland samples. DHT was administered to ovary-intact 6-week postnatal fem...

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Autores principales: Kanasaki, Haruhiko, Tumurbaatar, Tuvshintugs, Cairang, Zhouma, Tumurgan, Zolzaya, Oride, Aki, Okada, Hiroe, Kyo, Satoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616675/
https://www.ncbi.nlm.nih.gov/pubmed/36311909
http://dx.doi.org/10.1155/2022/9525227
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author Kanasaki, Haruhiko
Tumurbaatar, Tuvshintugs
Cairang, Zhouma
Tumurgan, Zolzaya
Oride, Aki
Okada, Hiroe
Kyo, Satoru
author_facet Kanasaki, Haruhiko
Tumurbaatar, Tuvshintugs
Cairang, Zhouma
Tumurgan, Zolzaya
Oride, Aki
Okada, Hiroe
Kyo, Satoru
author_sort Kanasaki, Haruhiko
collection PubMed
description Hyperandrogenism causes dysfunction of the hypothalamic–pituitary–gonadal (HPG) axis in reproductive women. In this study, we examined the effects of dihydrotestosterone (DHT) on characteristic changes in rat anterior pituitary gland samples. DHT was administered to ovary-intact 6-week postnatal female rats for 7 days, after which the anterior pituitary glands were examined and compared with those in control rats. Estrous cyclicity was not drastically disrupted by DHT treatment. Common gonadotropin α subunit (Cga), luteinizing hormone β subunit (Lhb), and follicle-stimulating hormone (FSH) β subunit (Fshb) gene expression levels were not modulated by DHT treatment, while prolactin (Prl) gene expression was significantly repressed by DHT. Gonadotropin-releasing hormone (GnRH) receptor (Gnrh-r) gene expression was significantly inhibited by DHT, whereas pituitary adenylate cyclase-activating polypeptide (PACAP) receptor (Pca1-r) gene expression was increased by DHT. Gene expression levels of the receptors encoded by thyrotropin-releasing hormone (Trh-r) and kisspeptin (Kiss1-r) genes were unchanged. Expression of inhibin α subunit (Inha) and activin βA subunits (Actba) within the pituitary was inhibited by DHT treatment, while activin B subunit (Actbb) and follistatin (Fst) gene expression was unchanged by DHT. In mouse pituitary gonadotroph LβT2 cells, DHT did not modulate the gene expression of Gnrh-r, but it inhibited the expression of Inha and Actba subunits within the LβT2 cells. In rat prolactin-producing GH3 cells, DHT did not modulate prolactin gene expression, but it increased Pac1-r gene expression. The present observations suggest that DHT directly or indirectly affects the anterior pituitary gland and induces characteristic changes in hormone-producing cells.
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spelling pubmed-96166752022-10-29 Impact of One-Week Administration of Dihydrotestosterone in Rat Anterior Pituitary Gland Kanasaki, Haruhiko Tumurbaatar, Tuvshintugs Cairang, Zhouma Tumurgan, Zolzaya Oride, Aki Okada, Hiroe Kyo, Satoru Int J Endocrinol Research Article Hyperandrogenism causes dysfunction of the hypothalamic–pituitary–gonadal (HPG) axis in reproductive women. In this study, we examined the effects of dihydrotestosterone (DHT) on characteristic changes in rat anterior pituitary gland samples. DHT was administered to ovary-intact 6-week postnatal female rats for 7 days, after which the anterior pituitary glands were examined and compared with those in control rats. Estrous cyclicity was not drastically disrupted by DHT treatment. Common gonadotropin α subunit (Cga), luteinizing hormone β subunit (Lhb), and follicle-stimulating hormone (FSH) β subunit (Fshb) gene expression levels were not modulated by DHT treatment, while prolactin (Prl) gene expression was significantly repressed by DHT. Gonadotropin-releasing hormone (GnRH) receptor (Gnrh-r) gene expression was significantly inhibited by DHT, whereas pituitary adenylate cyclase-activating polypeptide (PACAP) receptor (Pca1-r) gene expression was increased by DHT. Gene expression levels of the receptors encoded by thyrotropin-releasing hormone (Trh-r) and kisspeptin (Kiss1-r) genes were unchanged. Expression of inhibin α subunit (Inha) and activin βA subunits (Actba) within the pituitary was inhibited by DHT treatment, while activin B subunit (Actbb) and follistatin (Fst) gene expression was unchanged by DHT. In mouse pituitary gonadotroph LβT2 cells, DHT did not modulate the gene expression of Gnrh-r, but it inhibited the expression of Inha and Actba subunits within the LβT2 cells. In rat prolactin-producing GH3 cells, DHT did not modulate prolactin gene expression, but it increased Pac1-r gene expression. The present observations suggest that DHT directly or indirectly affects the anterior pituitary gland and induces characteristic changes in hormone-producing cells. Hindawi 2022-10-21 /pmc/articles/PMC9616675/ /pubmed/36311909 http://dx.doi.org/10.1155/2022/9525227 Text en Copyright © 2022 Haruhiko Kanasaki et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kanasaki, Haruhiko
Tumurbaatar, Tuvshintugs
Cairang, Zhouma
Tumurgan, Zolzaya
Oride, Aki
Okada, Hiroe
Kyo, Satoru
Impact of One-Week Administration of Dihydrotestosterone in Rat Anterior Pituitary Gland
title Impact of One-Week Administration of Dihydrotestosterone in Rat Anterior Pituitary Gland
title_full Impact of One-Week Administration of Dihydrotestosterone in Rat Anterior Pituitary Gland
title_fullStr Impact of One-Week Administration of Dihydrotestosterone in Rat Anterior Pituitary Gland
title_full_unstemmed Impact of One-Week Administration of Dihydrotestosterone in Rat Anterior Pituitary Gland
title_short Impact of One-Week Administration of Dihydrotestosterone in Rat Anterior Pituitary Gland
title_sort impact of one-week administration of dihydrotestosterone in rat anterior pituitary gland
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616675/
https://www.ncbi.nlm.nih.gov/pubmed/36311909
http://dx.doi.org/10.1155/2022/9525227
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