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DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential

Cells with latent stem ability can contribute to mammalian tissue regeneration after damage. Whether the central nervous system (CNS) harbors such cells remains controversial. Here, we report that DNGR-1 lineage tracing in mice identifies an ependymal cell subset, wherein resides latent regenerative...

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Autores principales: Frederico, Bruno, Martins, Isaura, Chapela, Diana, Gasparrini, Francesca, Chakravarty, Probir, Ackels, Tobias, Piot, Cécile, Almeida, Bruna, Carvalho, Joana, Ciccarelli, Alessandro, Peddie, Christopher J., Rogers, Neil, Briscoe, James, Guillemot, François, Schaefer, Andreas T., Saúde, Leonor, Reis e Sousa, Caetano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616800/
https://www.ncbi.nlm.nih.gov/pubmed/35998585
http://dx.doi.org/10.1016/j.devcel.2022.07.012
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author Frederico, Bruno
Martins, Isaura
Chapela, Diana
Gasparrini, Francesca
Chakravarty, Probir
Ackels, Tobias
Piot, Cécile
Almeida, Bruna
Carvalho, Joana
Ciccarelli, Alessandro
Peddie, Christopher J.
Rogers, Neil
Briscoe, James
Guillemot, François
Schaefer, Andreas T.
Saúde, Leonor
Reis e Sousa, Caetano
author_facet Frederico, Bruno
Martins, Isaura
Chapela, Diana
Gasparrini, Francesca
Chakravarty, Probir
Ackels, Tobias
Piot, Cécile
Almeida, Bruna
Carvalho, Joana
Ciccarelli, Alessandro
Peddie, Christopher J.
Rogers, Neil
Briscoe, James
Guillemot, François
Schaefer, Andreas T.
Saúde, Leonor
Reis e Sousa, Caetano
author_sort Frederico, Bruno
collection PubMed
description Cells with latent stem ability can contribute to mammalian tissue regeneration after damage. Whether the central nervous system (CNS) harbors such cells remains controversial. Here, we report that DNGR-1 lineage tracing in mice identifies an ependymal cell subset, wherein resides latent regenerative potential. We demonstrate that DNGR-1-lineage-traced ependymal cells arise early in embryogenesis (E11.5) and subsequently spread across the lining of cerebrospinal fluid (CSF)-filled compartments to form a contiguous sheet from the brain to the end of the spinal cord. In the steady state, these DNGR-1-traced cells are quiescent, committed to their ependymal cell fate, and do not contribute to neuronal or glial lineages. However, trans-differentiation can be induced in adult mice by CNS injury or in vitro by culture with suitable factors. Our findings highlight previously unappreciated ependymal cell heterogeneity and identify across the entire CNS an ependymal cell subset wherein resides damage-responsive neural stem cell potential.
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spelling pubmed-96168002022-10-31 DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential Frederico, Bruno Martins, Isaura Chapela, Diana Gasparrini, Francesca Chakravarty, Probir Ackels, Tobias Piot, Cécile Almeida, Bruna Carvalho, Joana Ciccarelli, Alessandro Peddie, Christopher J. Rogers, Neil Briscoe, James Guillemot, François Schaefer, Andreas T. Saúde, Leonor Reis e Sousa, Caetano Dev Cell Article Cells with latent stem ability can contribute to mammalian tissue regeneration after damage. Whether the central nervous system (CNS) harbors such cells remains controversial. Here, we report that DNGR-1 lineage tracing in mice identifies an ependymal cell subset, wherein resides latent regenerative potential. We demonstrate that DNGR-1-lineage-traced ependymal cells arise early in embryogenesis (E11.5) and subsequently spread across the lining of cerebrospinal fluid (CSF)-filled compartments to form a contiguous sheet from the brain to the end of the spinal cord. In the steady state, these DNGR-1-traced cells are quiescent, committed to their ependymal cell fate, and do not contribute to neuronal or glial lineages. However, trans-differentiation can be induced in adult mice by CNS injury or in vitro by culture with suitable factors. Our findings highlight previously unappreciated ependymal cell heterogeneity and identify across the entire CNS an ependymal cell subset wherein resides damage-responsive neural stem cell potential. Cell Press 2022-08-22 /pmc/articles/PMC9616800/ /pubmed/35998585 http://dx.doi.org/10.1016/j.devcel.2022.07.012 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Frederico, Bruno
Martins, Isaura
Chapela, Diana
Gasparrini, Francesca
Chakravarty, Probir
Ackels, Tobias
Piot, Cécile
Almeida, Bruna
Carvalho, Joana
Ciccarelli, Alessandro
Peddie, Christopher J.
Rogers, Neil
Briscoe, James
Guillemot, François
Schaefer, Andreas T.
Saúde, Leonor
Reis e Sousa, Caetano
DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential
title DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential
title_full DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential
title_fullStr DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential
title_full_unstemmed DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential
title_short DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential
title_sort dngr-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616800/
https://www.ncbi.nlm.nih.gov/pubmed/35998585
http://dx.doi.org/10.1016/j.devcel.2022.07.012
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