Cargando…
Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza
Flavonoid secondary metabolites can treat and prevent many diseases, but systematic studies on regulation of the biosynthesis of such metabolites in aboveground parts of Salvia miltiorrhiza are lacking. In this study, metabonomic and transcriptomic analyses of different S. miltiorrhiza phenotypes we...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616839/ https://www.ncbi.nlm.nih.gov/pubmed/36307498 http://dx.doi.org/10.1038/s41598-022-21517-5 |
_version_ | 1784820728275664896 |
---|---|
author | Yang, Hanting Li, Hongyan Li, Qian |
author_facet | Yang, Hanting Li, Hongyan Li, Qian |
author_sort | Yang, Hanting |
collection | PubMed |
description | Flavonoid secondary metabolites can treat and prevent many diseases, but systematic studies on regulation of the biosynthesis of such metabolites in aboveground parts of Salvia miltiorrhiza are lacking. In this study, metabonomic and transcriptomic analyses of different S. miltiorrhiza phenotypes were conducted to explore pathways of synthesis, catalysis, accumulation, and transport of the main flavonoid secondary metabolites regulating pigment accumulation. Tissue localization and quantitative analysis of flavonoid secondary metabolites were conducted by laser scanning confocal microscopy (LSCM). A total 3090 differentially expressed genes were obtained from 114,431 full-length unigenes in purple and green phenotypes, and 108 functional genes were involved in flavonoid biosynthesis. Five key phenylpropane structural genes (PAL, 4CL, ANS, 3AT, HCT) were highly differentially expressed, and four transcription factor genes (MYB, WRKY, bHLH, bZiP) were identified. In addition, six GST genes, nine ABC transporters, 22 MATE genes, and three SNARE genes were detected with key roles in flavonoid transport. According to LSCM, flavonoids were mainly distributed in epidermis, cortex, and collenchyma. Thus, comprehensive and systematic analyses were used to determine biosynthesis, accumulation, and transport of flavonoids in stems and leaves of different S. miltiorrhiza phenotypes. The findings will provide a reference for flavonoid production and cultivar selection. |
format | Online Article Text |
id | pubmed-9616839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96168392022-10-30 Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza Yang, Hanting Li, Hongyan Li, Qian Sci Rep Article Flavonoid secondary metabolites can treat and prevent many diseases, but systematic studies on regulation of the biosynthesis of such metabolites in aboveground parts of Salvia miltiorrhiza are lacking. In this study, metabonomic and transcriptomic analyses of different S. miltiorrhiza phenotypes were conducted to explore pathways of synthesis, catalysis, accumulation, and transport of the main flavonoid secondary metabolites regulating pigment accumulation. Tissue localization and quantitative analysis of flavonoid secondary metabolites were conducted by laser scanning confocal microscopy (LSCM). A total 3090 differentially expressed genes were obtained from 114,431 full-length unigenes in purple and green phenotypes, and 108 functional genes were involved in flavonoid biosynthesis. Five key phenylpropane structural genes (PAL, 4CL, ANS, 3AT, HCT) were highly differentially expressed, and four transcription factor genes (MYB, WRKY, bHLH, bZiP) were identified. In addition, six GST genes, nine ABC transporters, 22 MATE genes, and three SNARE genes were detected with key roles in flavonoid transport. According to LSCM, flavonoids were mainly distributed in epidermis, cortex, and collenchyma. Thus, comprehensive and systematic analyses were used to determine biosynthesis, accumulation, and transport of flavonoids in stems and leaves of different S. miltiorrhiza phenotypes. The findings will provide a reference for flavonoid production and cultivar selection. Nature Publishing Group UK 2022-10-28 /pmc/articles/PMC9616839/ /pubmed/36307498 http://dx.doi.org/10.1038/s41598-022-21517-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yang, Hanting Li, Hongyan Li, Qian Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza |
title | Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza |
title_full | Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza |
title_fullStr | Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza |
title_full_unstemmed | Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza |
title_short | Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza |
title_sort | biosynthetic regulatory network of flavonoid metabolites in stems and leaves of salvia miltiorrhiza |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616839/ https://www.ncbi.nlm.nih.gov/pubmed/36307498 http://dx.doi.org/10.1038/s41598-022-21517-5 |
work_keys_str_mv | AT yanghanting biosyntheticregulatorynetworkofflavonoidmetabolitesinstemsandleavesofsalviamiltiorrhiza AT lihongyan biosyntheticregulatorynetworkofflavonoidmetabolitesinstemsandleavesofsalviamiltiorrhiza AT liqian biosyntheticregulatorynetworkofflavonoidmetabolitesinstemsandleavesofsalviamiltiorrhiza |