Cargando…

Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients

Perturbations in the gut microbiome have been associated with colorectal cancer (CRC), with the colonic overabundance of Fusobacterium nucleatum shown as the most consistent marker. Despite its significance in the promotion of CRC, genomic studies of Fusobacterium is limited. We enrolled 43 Vietname...

Descripción completa

Detalles Bibliográficos
Autores principales: Tran, Hoang N. H., Thu, Trang Nguyen Hoang, Nguyen, Phu Huu, Vo, Chi Nguyen, Doan, Khanh Van, Nguyen Ngoc Minh, Chau, Nguyen, Ngoc Tuan, Ta, Van Ngoc Duc, Vu, Khuong An, Hua, Thanh Danh, Nguyen, To Nguyen Thi, Van, Tan Trinh, Pham Duc, Trung, Duong, Ba Lap, Nguyen, Phuc Minh, Hoang, Vinh Chuc, Pham, Duy Thanh, Thwaites, Guy E., Hall, Lindsay J., Slade, Daniel J., Baker, Stephen, Tran, Vinh Hung, Chung The, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616903/
https://www.ncbi.nlm.nih.gov/pubmed/36307484
http://dx.doi.org/10.1038/s41522-022-00351-7
_version_ 1784820741498208256
author Tran, Hoang N. H.
Thu, Trang Nguyen Hoang
Nguyen, Phu Huu
Vo, Chi Nguyen
Doan, Khanh Van
Nguyen Ngoc Minh, Chau
Nguyen, Ngoc Tuan
Ta, Van Ngoc Duc
Vu, Khuong An
Hua, Thanh Danh
Nguyen, To Nguyen Thi
Van, Tan Trinh
Pham Duc, Trung
Duong, Ba Lap
Nguyen, Phuc Minh
Hoang, Vinh Chuc
Pham, Duy Thanh
Thwaites, Guy E.
Hall, Lindsay J.
Slade, Daniel J.
Baker, Stephen
Tran, Vinh Hung
Chung The, Hao
author_facet Tran, Hoang N. H.
Thu, Trang Nguyen Hoang
Nguyen, Phu Huu
Vo, Chi Nguyen
Doan, Khanh Van
Nguyen Ngoc Minh, Chau
Nguyen, Ngoc Tuan
Ta, Van Ngoc Duc
Vu, Khuong An
Hua, Thanh Danh
Nguyen, To Nguyen Thi
Van, Tan Trinh
Pham Duc, Trung
Duong, Ba Lap
Nguyen, Phuc Minh
Hoang, Vinh Chuc
Pham, Duy Thanh
Thwaites, Guy E.
Hall, Lindsay J.
Slade, Daniel J.
Baker, Stephen
Tran, Vinh Hung
Chung The, Hao
author_sort Tran, Hoang N. H.
collection PubMed
description Perturbations in the gut microbiome have been associated with colorectal cancer (CRC), with the colonic overabundance of Fusobacterium nucleatum shown as the most consistent marker. Despite its significance in the promotion of CRC, genomic studies of Fusobacterium is limited. We enrolled 43 Vietnamese CRC patients and 25 participants with non-cancerous colorectal polyps to study the colonic microbiomes and genomic diversity of Fusobacterium in this population, using a combination of 16S rRNA gene profiling, anaerobic microbiology, and whole genome analysis. Oral bacteria, including F. nucleatum and Leptotrichia, were significantly more abundant in the tumour microbiomes. We obtained 53 Fusobacterium genomes, representing 26 strains, from the saliva, tumour and non-tumour tissues of six CRC patients. Isolates from the gut belonged to diverse F. nucleatum subspecies (nucleatum, animalis, vincentii, polymorphum) and a potential new subspecies of Fusobacterium periodonticum. The Fusobacterium population within each individual was distinct and in some cases diverse, with minimal intra-clonal variation. Phylogenetic analyses showed that within four individuals, tumour-associated Fusobacterium were clonal to those isolated from non-tumour tissues. Genes encoding major virulence factors (Fap2 and RadD) showed evidence of horizontal gene transfer. Our work provides a framework to understand the genomic diversity of Fusobacterium within the CRC patients, which can be exploited for the development of CRC diagnostic and therapeutic options targeting this oncobacterium.
format Online
Article
Text
id pubmed-9616903
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-96169032022-10-30 Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients Tran, Hoang N. H. Thu, Trang Nguyen Hoang Nguyen, Phu Huu Vo, Chi Nguyen Doan, Khanh Van Nguyen Ngoc Minh, Chau Nguyen, Ngoc Tuan Ta, Van Ngoc Duc Vu, Khuong An Hua, Thanh Danh Nguyen, To Nguyen Thi Van, Tan Trinh Pham Duc, Trung Duong, Ba Lap Nguyen, Phuc Minh Hoang, Vinh Chuc Pham, Duy Thanh Thwaites, Guy E. Hall, Lindsay J. Slade, Daniel J. Baker, Stephen Tran, Vinh Hung Chung The, Hao NPJ Biofilms Microbiomes Article Perturbations in the gut microbiome have been associated with colorectal cancer (CRC), with the colonic overabundance of Fusobacterium nucleatum shown as the most consistent marker. Despite its significance in the promotion of CRC, genomic studies of Fusobacterium is limited. We enrolled 43 Vietnamese CRC patients and 25 participants with non-cancerous colorectal polyps to study the colonic microbiomes and genomic diversity of Fusobacterium in this population, using a combination of 16S rRNA gene profiling, anaerobic microbiology, and whole genome analysis. Oral bacteria, including F. nucleatum and Leptotrichia, were significantly more abundant in the tumour microbiomes. We obtained 53 Fusobacterium genomes, representing 26 strains, from the saliva, tumour and non-tumour tissues of six CRC patients. Isolates from the gut belonged to diverse F. nucleatum subspecies (nucleatum, animalis, vincentii, polymorphum) and a potential new subspecies of Fusobacterium periodonticum. The Fusobacterium population within each individual was distinct and in some cases diverse, with minimal intra-clonal variation. Phylogenetic analyses showed that within four individuals, tumour-associated Fusobacterium were clonal to those isolated from non-tumour tissues. Genes encoding major virulence factors (Fap2 and RadD) showed evidence of horizontal gene transfer. Our work provides a framework to understand the genomic diversity of Fusobacterium within the CRC patients, which can be exploited for the development of CRC diagnostic and therapeutic options targeting this oncobacterium. Nature Publishing Group UK 2022-10-29 /pmc/articles/PMC9616903/ /pubmed/36307484 http://dx.doi.org/10.1038/s41522-022-00351-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Tran, Hoang N. H.
Thu, Trang Nguyen Hoang
Nguyen, Phu Huu
Vo, Chi Nguyen
Doan, Khanh Van
Nguyen Ngoc Minh, Chau
Nguyen, Ngoc Tuan
Ta, Van Ngoc Duc
Vu, Khuong An
Hua, Thanh Danh
Nguyen, To Nguyen Thi
Van, Tan Trinh
Pham Duc, Trung
Duong, Ba Lap
Nguyen, Phuc Minh
Hoang, Vinh Chuc
Pham, Duy Thanh
Thwaites, Guy E.
Hall, Lindsay J.
Slade, Daniel J.
Baker, Stephen
Tran, Vinh Hung
Chung The, Hao
Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients
title Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients
title_full Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients
title_fullStr Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients
title_full_unstemmed Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients
title_short Tumour microbiomes and Fusobacterium genomics in Vietnamese colorectal cancer patients
title_sort tumour microbiomes and fusobacterium genomics in vietnamese colorectal cancer patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9616903/
https://www.ncbi.nlm.nih.gov/pubmed/36307484
http://dx.doi.org/10.1038/s41522-022-00351-7
work_keys_str_mv AT tranhoangnh tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT thutrangnguyenhoang tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT nguyenphuhuu tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT vochinguyen tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT doankhanhvan tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT nguyenngocminhchau tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT nguyenngoctuan tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT tavanngocduc tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT vukhuongan tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT huathanhdanh tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT nguyentonguyenthi tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT vantantrinh tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT phamductrung tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT duongbalap tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT nguyenphucminh tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT hoangvinhchuc tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT phamduythanh tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT thwaitesguye tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT halllindsayj tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT sladedanielj tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT bakerstephen tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT tranvinhhung tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients
AT chungthehao tumourmicrobiomesandfusobacteriumgenomicsinvietnamesecolorectalcancerpatients