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Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines

OBJECTIVE: The human large intergenic non-coding RNA-regulator of reprogramming program (linc-ROR) is known as a stem cell specific linc-RNA. linc-ROR counteracts differentiation via sequestering microRNA-145 (miR-145) that targets OCT4 transcript. Despite the research on the expression and function...

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Autores principales: Mirzadeh Azad, Fatemeh, Taheri Bajgan, Elham, Naeli, Parisa, Rudov, Alexander, Bagheri Moghadam, Mahrokh, Sadat Akhtar, Mozhgan, Gholipour, Akram, Mowla, Seyed Javad, Malakootian, Mahshid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617025/
https://www.ncbi.nlm.nih.gov/pubmed/36259474
http://dx.doi.org/10.22074/cellj.2022.8205
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author Mirzadeh Azad, Fatemeh
Taheri Bajgan, Elham
Naeli, Parisa
Rudov, Alexander
Bagheri Moghadam, Mahrokh
Sadat Akhtar, Mozhgan
Gholipour, Akram
Mowla, Seyed Javad
Malakootian, Mahshid
author_facet Mirzadeh Azad, Fatemeh
Taheri Bajgan, Elham
Naeli, Parisa
Rudov, Alexander
Bagheri Moghadam, Mahrokh
Sadat Akhtar, Mozhgan
Gholipour, Akram
Mowla, Seyed Javad
Malakootian, Mahshid
author_sort Mirzadeh Azad, Fatemeh
collection PubMed
description OBJECTIVE: The human large intergenic non-coding RNA-regulator of reprogramming program (linc-ROR) is known as a stem cell specific linc-RNA. linc-ROR counteracts differentiation via sequestering microRNA-145 (miR-145) that targets OCT4 transcript. Despite the research on the expression and function, the exact structure of Linc-ROR transcripts is not clear. Considering the contribution of alternative splicing in transcripts structures and function, identifying different spliced variants of linc-ROR is necessary for further functional analyses. We aimed to find the alternatively spliced transcripts of linc-ROR and investigate their expression pattern in stem and cancer cell lines and during neural differentiation of NT2 cells as a model for understanding linc-ROR role in stem cell and differentiation. MATERIALS AND METHODS: In this experimental study, linc-ROR locus was scanned for identifying novel exons. Different primer sets were used to detect new spliced variants by reverse transcription polymerase chain reaction (RT-PCR) and direct sequencing. Quantitative PCR (qPCR) and RT-PCR were employed to profile expression of linc-ROR transcripts in different cell lines and during neural differentiation of stem cells. RESULTS: We could discover 13 novel spliced variants of linc-ROR harboring unique array of exons. Our work uncovered six novel exons, some of which were the product of exonized transposable elements. Monitoring expression profile of the linc-ROR spliced variants in a panel of pluripotent and non-pluripotent cells exhibited that all transcripts were primarily expressed in pluripotent cells. Moreover, the examined linc-ROR spliced variants showed a similar down- regulation during neural differentiation of NT2 cells. CONCLUSION: Altogether, our data showed despite the difference in the structure and composition of exons, various spliced variants of linc-ROR showed similar expression pattern in stem cells and through differentiation.
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spelling pubmed-96170252022-11-16 Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines Mirzadeh Azad, Fatemeh Taheri Bajgan, Elham Naeli, Parisa Rudov, Alexander Bagheri Moghadam, Mahrokh Sadat Akhtar, Mozhgan Gholipour, Akram Mowla, Seyed Javad Malakootian, Mahshid Cell J Original Article OBJECTIVE: The human large intergenic non-coding RNA-regulator of reprogramming program (linc-ROR) is known as a stem cell specific linc-RNA. linc-ROR counteracts differentiation via sequestering microRNA-145 (miR-145) that targets OCT4 transcript. Despite the research on the expression and function, the exact structure of Linc-ROR transcripts is not clear. Considering the contribution of alternative splicing in transcripts structures and function, identifying different spliced variants of linc-ROR is necessary for further functional analyses. We aimed to find the alternatively spliced transcripts of linc-ROR and investigate their expression pattern in stem and cancer cell lines and during neural differentiation of NT2 cells as a model for understanding linc-ROR role in stem cell and differentiation. MATERIALS AND METHODS: In this experimental study, linc-ROR locus was scanned for identifying novel exons. Different primer sets were used to detect new spliced variants by reverse transcription polymerase chain reaction (RT-PCR) and direct sequencing. Quantitative PCR (qPCR) and RT-PCR were employed to profile expression of linc-ROR transcripts in different cell lines and during neural differentiation of stem cells. RESULTS: We could discover 13 novel spliced variants of linc-ROR harboring unique array of exons. Our work uncovered six novel exons, some of which were the product of exonized transposable elements. Monitoring expression profile of the linc-ROR spliced variants in a panel of pluripotent and non-pluripotent cells exhibited that all transcripts were primarily expressed in pluripotent cells. Moreover, the examined linc-ROR spliced variants showed a similar down- regulation during neural differentiation of NT2 cells. CONCLUSION: Altogether, our data showed despite the difference in the structure and composition of exons, various spliced variants of linc-ROR showed similar expression pattern in stem cells and through differentiation. Royan Institute 2022-10 2022-10-09 /pmc/articles/PMC9617025/ /pubmed/36259474 http://dx.doi.org/10.22074/cellj.2022.8205 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Mirzadeh Azad, Fatemeh
Taheri Bajgan, Elham
Naeli, Parisa
Rudov, Alexander
Bagheri Moghadam, Mahrokh
Sadat Akhtar, Mozhgan
Gholipour, Akram
Mowla, Seyed Javad
Malakootian, Mahshid
Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines
title Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines
title_full Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines
title_fullStr Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines
title_full_unstemmed Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines
title_short Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines
title_sort differential expression pattern of linc-ror spliced variants in pluripotent and non-pluripotent cell lines
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617025/
https://www.ncbi.nlm.nih.gov/pubmed/36259474
http://dx.doi.org/10.22074/cellj.2022.8205
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