Cargando…
Effect of Single Embryo Blastomere Biopsy from Human Frozen Embryos on Assisted Reproductive Outcomes
OBJECTIVE: Preimplantation genetic testing for aneuploidies (PGT-A) is used to determine chromosomal normality and achieve a successful live birth in infertile couples. There is a possible correlation between chromosomal aneuploidy, embryo development and pregnancy rate. This study evaluated the inf...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617026/ https://www.ncbi.nlm.nih.gov/pubmed/36259481 http://dx.doi.org/10.22074/cellj.2022.8328 |
_version_ | 1784820762214924288 |
---|---|
author | Aghajani, Shahrzad Salehzadeh, Ali Ghasemian, Fatemeh Mehrafza, Marzieh Hosseini, Ahmad |
author_facet | Aghajani, Shahrzad Salehzadeh, Ali Ghasemian, Fatemeh Mehrafza, Marzieh Hosseini, Ahmad |
author_sort | Aghajani, Shahrzad |
collection | PubMed |
description | OBJECTIVE: Preimplantation genetic testing for aneuploidies (PGT-A) is used to determine chromosomal normality and achieve a successful live birth in infertile couples. There is a possible correlation between chromosomal aneuploidy, embryo development and pregnancy rate. This study evaluated the influence of single blastomere biopsy (SBB) on embryo development and pregnancy rates during frozen embryo transfer (FET) and fresh cycles. MATERIALS AND METHODS: This quasi-experimental study evaluated 115 intracytoplasmic sperm injection (ICSI) cycles, including 443 embryos (6-8 cells) with a grade A on day three, following PGT-A in the fresh or FET cycles from February 2018 to June 2020. In addition, the fresh cycles without PGT were included as a control group (n=166 embryos). SBB was done on day three and was grouped as FET-PGT (n=149) and the fresh-PGT (n=128). RESULTS: There is a more aneuploidy rate in the FET-PGT group compared to the fresh-PGT cycle (36.60% vs. 20.38%, P<0.001). There is a rate of higher development and blastocyst in the control group. While the embryos of PGT groups showed higher degrees of expansion (expansion 5) on day five. 8.6, 8.59, and 9.37% of expansion 3, 4, and 5 in the fresh-PGT embryos, 12.58, 2.78, and 14.84% of expansion 3, 4, and 5 in the FET-PGD embryos compared to 10.84and 33.73% of expansion 3 and 4 in the control group (without expansion 5; P<0.001). There was no significant relationship between 13, 18, and 21 chromosome aneuploidies with blastocyst development competence among the groups (P<0.1). Following embryo transfer (n=97), the spontaneous abortion rate was higher in the FET-PGT cycles compared to the fresh-PGT and control groups (50 vs. 22 and 11%, respectively; P<0.04). CONCLUSION: The process of SBB following vitrification significantly decreased embryo development and pregnancy outcomes. Therefore, a morphological analysis could not be reliable in selecting chromosomally normal embryos. |
format | Online Article Text |
id | pubmed-9617026 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-96170262022-11-16 Effect of Single Embryo Blastomere Biopsy from Human Frozen Embryos on Assisted Reproductive Outcomes Aghajani, Shahrzad Salehzadeh, Ali Ghasemian, Fatemeh Mehrafza, Marzieh Hosseini, Ahmad Cell J Original Article OBJECTIVE: Preimplantation genetic testing for aneuploidies (PGT-A) is used to determine chromosomal normality and achieve a successful live birth in infertile couples. There is a possible correlation between chromosomal aneuploidy, embryo development and pregnancy rate. This study evaluated the influence of single blastomere biopsy (SBB) on embryo development and pregnancy rates during frozen embryo transfer (FET) and fresh cycles. MATERIALS AND METHODS: This quasi-experimental study evaluated 115 intracytoplasmic sperm injection (ICSI) cycles, including 443 embryos (6-8 cells) with a grade A on day three, following PGT-A in the fresh or FET cycles from February 2018 to June 2020. In addition, the fresh cycles without PGT were included as a control group (n=166 embryos). SBB was done on day three and was grouped as FET-PGT (n=149) and the fresh-PGT (n=128). RESULTS: There is a more aneuploidy rate in the FET-PGT group compared to the fresh-PGT cycle (36.60% vs. 20.38%, P<0.001). There is a rate of higher development and blastocyst in the control group. While the embryos of PGT groups showed higher degrees of expansion (expansion 5) on day five. 8.6, 8.59, and 9.37% of expansion 3, 4, and 5 in the fresh-PGT embryos, 12.58, 2.78, and 14.84% of expansion 3, 4, and 5 in the FET-PGD embryos compared to 10.84and 33.73% of expansion 3 and 4 in the control group (without expansion 5; P<0.001). There was no significant relationship between 13, 18, and 21 chromosome aneuploidies with blastocyst development competence among the groups (P<0.1). Following embryo transfer (n=97), the spontaneous abortion rate was higher in the FET-PGT cycles compared to the fresh-PGT and control groups (50 vs. 22 and 11%, respectively; P<0.04). CONCLUSION: The process of SBB following vitrification significantly decreased embryo development and pregnancy outcomes. Therefore, a morphological analysis could not be reliable in selecting chromosomally normal embryos. Royan Institute 2022-10 2022-10-09 /pmc/articles/PMC9617026/ /pubmed/36259481 http://dx.doi.org/10.22074/cellj.2022.8328 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Aghajani, Shahrzad Salehzadeh, Ali Ghasemian, Fatemeh Mehrafza, Marzieh Hosseini, Ahmad Effect of Single Embryo Blastomere Biopsy from Human Frozen Embryos on Assisted Reproductive Outcomes |
title | Effect of Single Embryo Blastomere Biopsy from Human Frozen
Embryos on Assisted Reproductive Outcomes |
title_full | Effect of Single Embryo Blastomere Biopsy from Human Frozen
Embryos on Assisted Reproductive Outcomes |
title_fullStr | Effect of Single Embryo Blastomere Biopsy from Human Frozen
Embryos on Assisted Reproductive Outcomes |
title_full_unstemmed | Effect of Single Embryo Blastomere Biopsy from Human Frozen
Embryos on Assisted Reproductive Outcomes |
title_short | Effect of Single Embryo Blastomere Biopsy from Human Frozen
Embryos on Assisted Reproductive Outcomes |
title_sort | effect of single embryo blastomere biopsy from human frozen
embryos on assisted reproductive outcomes |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617026/ https://www.ncbi.nlm.nih.gov/pubmed/36259481 http://dx.doi.org/10.22074/cellj.2022.8328 |
work_keys_str_mv | AT aghajanishahrzad effectofsingleembryoblastomerebiopsyfromhumanfrozenembryosonassistedreproductiveoutcomes AT salehzadehali effectofsingleembryoblastomerebiopsyfromhumanfrozenembryosonassistedreproductiveoutcomes AT ghasemianfatemeh effectofsingleembryoblastomerebiopsyfromhumanfrozenembryosonassistedreproductiveoutcomes AT mehrafzamarzieh effectofsingleembryoblastomerebiopsyfromhumanfrozenembryosonassistedreproductiveoutcomes AT hosseiniahmad effectofsingleembryoblastomerebiopsyfromhumanfrozenembryosonassistedreproductiveoutcomes |