Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells

Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures. The present protocol defines a specific strategy to amplify BRD4-L, followed by the successful cloning of the gene into an overexpression vecto...

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Detalles Bibliográficos
Autores principales: Drumond-Bock, Ana Luiza, Cybula, Magdalena, Wang, Luyao, Wang, Lin, Bieniasz, Magdalena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617197/
https://www.ncbi.nlm.nih.gov/pubmed/36317179
http://dx.doi.org/10.1016/j.xpro.2022.101785

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617197/
https://www.ncbi.nlm.nih.gov/pubmed/36317179
http://dx.doi.org/10.1016/j.xpro.2022.101785

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