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Cloning BRD4 long isoform into overexpression vectors for stable overexpression of BRD4-L in mammalian cells
Molecular cloning of BRD4-L is a challenging technique, because the DNA insert is formed by a long, GC-rich sequence, which folds into secondary structures. The present protocol defines a specific strategy to amplify BRD4-L, followed by the successful cloning of the gene into an overexpression vecto...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617197/ https://www.ncbi.nlm.nih.gov/pubmed/36317179 http://dx.doi.org/10.1016/j.xpro.2022.101785 |