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CRISPR-Cas9-induced gene knockout in zebrafish

The application of CRISPR has greatly facilitated genotype-phenotype studies of human disease models. In this protocol, we describe CRISPR-Cas9-induced gene knockout in zebrafish, utilizing purified Cas9 protein and in vitro-transcribed sgRNA. This protocol targets the PHLPP1 gene in an Indian wild-...

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Detalles Bibliográficos
Autores principales:	Medishetti, Raghavender, Balamurugan, Keerthana, Yadavalli, Krishnaveni, Rani, Rita, Sevilimedu, Aarti, Challa, Anil Kumar, Parsa, Kishore, Chatti, Kiranam
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9617198/ https://www.ncbi.nlm.nih.gov/pubmed/36317180 http://dx.doi.org/10.1016/j.xpro.2022.101779

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