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Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis
The high level of reactive oxygen species (ROS) in the rheumatoid arthritis (RA) microenvironment (RAM) and its persistent inflammatory nature can promote damage to joints, bones, and the synovium. Targeting strategies that integrate effective RAM regulation with imaging-based monitoring could lead...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618076/ https://www.ncbi.nlm.nih.gov/pubmed/36256822 http://dx.doi.org/10.1073/pnas.2213373119 |
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author | Chen, Jianhai Zeng, Silue Xue, Qiang Hong, Yuchuan Liu, Liangjian Song, Liang Fang, Chihua Zhang, Hai Wang, Benguo Sedgwick, Adam C. Zhang, Peng Sessler, Jonathan L. Liu, Chengbo Chen, Jingqin |
author_facet | Chen, Jianhai Zeng, Silue Xue, Qiang Hong, Yuchuan Liu, Liangjian Song, Liang Fang, Chihua Zhang, Hai Wang, Benguo Sedgwick, Adam C. Zhang, Peng Sessler, Jonathan L. Liu, Chengbo Chen, Jingqin |
author_sort | Chen, Jianhai |
collection | PubMed |
description | The high level of reactive oxygen species (ROS) in the rheumatoid arthritis (RA) microenvironment (RAM) and its persistent inflammatory nature can promote damage to joints, bones, and the synovium. Targeting strategies that integrate effective RAM regulation with imaging-based monitoring could lead to improvements in the diagnosis and treatment of RA. Here, we report the combined use of small interfering RNAs (siRNAs(T/I)) and Prussian blue nanoparticles (PBNPs) to silence the expression of proinflammatory cytokines TNF-α/IL-6 and scavenge the ROS associated with RAM. To enhance the in vitro and in vivo biological stability, biocompatibility, and targeting capability of the siRNAs(T/I) and PBNPs, macrophage membrane vesicles were used to prepare biomimetic nanoparticles, M@P-siRNAs(T/I). The resulting constructs were found to suppress tumor necrosis factor-α/interleukin-6 expression and overcome the hypoxic nature of RAM, thus alleviating RA-induced joint damage in a mouse model. The M@P-siRNAs(T/I) of this study could be monitored via near-infrared photoacoustic (PA) imaging. Moreover, multispectral PA imaging without the need for labeling permitted the real-time evaluation of M@P-siRNAs(T/I) as a putative RA treatment. Clinical microcomputed tomography and histological analysis confirmed the effectiveness of the treatment. We thus suggest that macrophage-biomimetic M@P-siRNAs(T/I) and their analogs assisted by PA imaging could provide a new strategy for RA diagnosis, treatment, and monitoring. |
format | Online Article Text |
id | pubmed-9618076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-96180762023-04-18 Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis Chen, Jianhai Zeng, Silue Xue, Qiang Hong, Yuchuan Liu, Liangjian Song, Liang Fang, Chihua Zhang, Hai Wang, Benguo Sedgwick, Adam C. Zhang, Peng Sessler, Jonathan L. Liu, Chengbo Chen, Jingqin Proc Natl Acad Sci U S A Physical Sciences The high level of reactive oxygen species (ROS) in the rheumatoid arthritis (RA) microenvironment (RAM) and its persistent inflammatory nature can promote damage to joints, bones, and the synovium. Targeting strategies that integrate effective RAM regulation with imaging-based monitoring could lead to improvements in the diagnosis and treatment of RA. Here, we report the combined use of small interfering RNAs (siRNAs(T/I)) and Prussian blue nanoparticles (PBNPs) to silence the expression of proinflammatory cytokines TNF-α/IL-6 and scavenge the ROS associated with RAM. To enhance the in vitro and in vivo biological stability, biocompatibility, and targeting capability of the siRNAs(T/I) and PBNPs, macrophage membrane vesicles were used to prepare biomimetic nanoparticles, M@P-siRNAs(T/I). The resulting constructs were found to suppress tumor necrosis factor-α/interleukin-6 expression and overcome the hypoxic nature of RAM, thus alleviating RA-induced joint damage in a mouse model. The M@P-siRNAs(T/I) of this study could be monitored via near-infrared photoacoustic (PA) imaging. Moreover, multispectral PA imaging without the need for labeling permitted the real-time evaluation of M@P-siRNAs(T/I) as a putative RA treatment. Clinical microcomputed tomography and histological analysis confirmed the effectiveness of the treatment. We thus suggest that macrophage-biomimetic M@P-siRNAs(T/I) and their analogs assisted by PA imaging could provide a new strategy for RA diagnosis, treatment, and monitoring. National Academy of Sciences 2022-10-18 2022-10-25 /pmc/articles/PMC9618076/ /pubmed/36256822 http://dx.doi.org/10.1073/pnas.2213373119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Physical Sciences Chen, Jianhai Zeng, Silue Xue, Qiang Hong, Yuchuan Liu, Liangjian Song, Liang Fang, Chihua Zhang, Hai Wang, Benguo Sedgwick, Adam C. Zhang, Peng Sessler, Jonathan L. Liu, Chengbo Chen, Jingqin Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis |
title | Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis |
title_full | Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis |
title_fullStr | Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis |
title_full_unstemmed | Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis |
title_short | Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis |
title_sort | photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis |
topic | Physical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618076/ https://www.ncbi.nlm.nih.gov/pubmed/36256822 http://dx.doi.org/10.1073/pnas.2213373119 |
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