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Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis

The high level of reactive oxygen species (ROS) in the rheumatoid arthritis (RA) microenvironment (RAM) and its persistent inflammatory nature can promote damage to joints, bones, and the synovium. Targeting strategies that integrate effective RAM regulation with imaging-based monitoring could lead...

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Autores principales: Chen, Jianhai, Zeng, Silue, Xue, Qiang, Hong, Yuchuan, Liu, Liangjian, Song, Liang, Fang, Chihua, Zhang, Hai, Wang, Benguo, Sedgwick, Adam C., Zhang, Peng, Sessler, Jonathan L., Liu, Chengbo, Chen, Jingqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618076/
https://www.ncbi.nlm.nih.gov/pubmed/36256822
http://dx.doi.org/10.1073/pnas.2213373119
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author Chen, Jianhai
Zeng, Silue
Xue, Qiang
Hong, Yuchuan
Liu, Liangjian
Song, Liang
Fang, Chihua
Zhang, Hai
Wang, Benguo
Sedgwick, Adam C.
Zhang, Peng
Sessler, Jonathan L.
Liu, Chengbo
Chen, Jingqin
author_facet Chen, Jianhai
Zeng, Silue
Xue, Qiang
Hong, Yuchuan
Liu, Liangjian
Song, Liang
Fang, Chihua
Zhang, Hai
Wang, Benguo
Sedgwick, Adam C.
Zhang, Peng
Sessler, Jonathan L.
Liu, Chengbo
Chen, Jingqin
author_sort Chen, Jianhai
collection PubMed
description The high level of reactive oxygen species (ROS) in the rheumatoid arthritis (RA) microenvironment (RAM) and its persistent inflammatory nature can promote damage to joints, bones, and the synovium. Targeting strategies that integrate effective RAM regulation with imaging-based monitoring could lead to improvements in the diagnosis and treatment of RA. Here, we report the combined use of small interfering RNAs (siRNAs(T/I)) and Prussian blue nanoparticles (PBNPs) to silence the expression of proinflammatory cytokines TNF-α/IL-6 and scavenge the ROS associated with RAM. To enhance the in vitro and in vivo biological stability, biocompatibility, and targeting capability of the siRNAs(T/I) and PBNPs, macrophage membrane vesicles were used to prepare biomimetic nanoparticles, M@P-siRNAs(T/I). The resulting constructs were found to suppress tumor necrosis factor-α/interleukin-6 expression and overcome the hypoxic nature of RAM, thus alleviating RA-induced joint damage in a mouse model. The M@P-siRNAs(T/I) of this study could be monitored via near-infrared photoacoustic (PA) imaging. Moreover, multispectral PA imaging without the need for labeling permitted the real-time evaluation of M@P-siRNAs(T/I) as a putative RA treatment. Clinical microcomputed tomography and histological analysis confirmed the effectiveness of the treatment. We thus suggest that macrophage-biomimetic M@P-siRNAs(T/I) and their analogs assisted by PA imaging could provide a new strategy for RA diagnosis, treatment, and monitoring.
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spelling pubmed-96180762023-04-18 Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis Chen, Jianhai Zeng, Silue Xue, Qiang Hong, Yuchuan Liu, Liangjian Song, Liang Fang, Chihua Zhang, Hai Wang, Benguo Sedgwick, Adam C. Zhang, Peng Sessler, Jonathan L. Liu, Chengbo Chen, Jingqin Proc Natl Acad Sci U S A Physical Sciences The high level of reactive oxygen species (ROS) in the rheumatoid arthritis (RA) microenvironment (RAM) and its persistent inflammatory nature can promote damage to joints, bones, and the synovium. Targeting strategies that integrate effective RAM regulation with imaging-based monitoring could lead to improvements in the diagnosis and treatment of RA. Here, we report the combined use of small interfering RNAs (siRNAs(T/I)) and Prussian blue nanoparticles (PBNPs) to silence the expression of proinflammatory cytokines TNF-α/IL-6 and scavenge the ROS associated with RAM. To enhance the in vitro and in vivo biological stability, biocompatibility, and targeting capability of the siRNAs(T/I) and PBNPs, macrophage membrane vesicles were used to prepare biomimetic nanoparticles, M@P-siRNAs(T/I). The resulting constructs were found to suppress tumor necrosis factor-α/interleukin-6 expression and overcome the hypoxic nature of RAM, thus alleviating RA-induced joint damage in a mouse model. The M@P-siRNAs(T/I) of this study could be monitored via near-infrared photoacoustic (PA) imaging. Moreover, multispectral PA imaging without the need for labeling permitted the real-time evaluation of M@P-siRNAs(T/I) as a putative RA treatment. Clinical microcomputed tomography and histological analysis confirmed the effectiveness of the treatment. We thus suggest that macrophage-biomimetic M@P-siRNAs(T/I) and their analogs assisted by PA imaging could provide a new strategy for RA diagnosis, treatment, and monitoring. National Academy of Sciences 2022-10-18 2022-10-25 /pmc/articles/PMC9618076/ /pubmed/36256822 http://dx.doi.org/10.1073/pnas.2213373119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Physical Sciences
Chen, Jianhai
Zeng, Silue
Xue, Qiang
Hong, Yuchuan
Liu, Liangjian
Song, Liang
Fang, Chihua
Zhang, Hai
Wang, Benguo
Sedgwick, Adam C.
Zhang, Peng
Sessler, Jonathan L.
Liu, Chengbo
Chen, Jingqin
Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis
title Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis
title_full Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis
title_fullStr Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis
title_full_unstemmed Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis
title_short Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis
title_sort photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis
topic Physical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618076/
https://www.ncbi.nlm.nih.gov/pubmed/36256822
http://dx.doi.org/10.1073/pnas.2213373119
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