Cargando…

Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

BACKGROUND: In the field of nanoscience there is an increasing interest to follow dynamics of nanoparticles (NP) in cells with an emphasis on endo-lysosomal pathways and long-term NP fate. During our research on this topic, we encountered several pitfalls, which can bias the experimental outcome. We...

Descripción completa

Detalles Bibliográficos
Autores principales:	Moreno-Echeverri, Aura Maria, Susnik, Eva, Vanhecke, Dimitri, Taladriz-Blanco, Patricia, Balog, Sandor, Petri-Fink, Alke, Rothen-Rutishauser, Barbara
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618187/ https://www.ncbi.nlm.nih.gov/pubmed/36309696 http://dx.doi.org/10.1186/s12951-022-01670-9

Ejemplares similares

Increased Uptake of Silica Nanoparticles in Inflamed Macrophages but Not upon Co-Exposure to Micron-Sized Particles
por: Susnik, Eva, et al.
Publicado: (2020)

The Functions of Cholera Toxin Subunit B as a Modulator of Silica Nanoparticle Endocytosis
por: Susnik, Eva, et al.
Publicado: (2023)

Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells
por: Susnik, Eva, et al.
Publicado: (2023)

Characterization of the Shape Anisotropy of Superparamagnetic Iron Oxide Nanoparticles during Thermal Decomposition
por: Vanhecke, Dimitri, et al.
Publicado: (2020)

Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine
por: Sousa de Almeida, Mauro, et al.
Publicado: (2021)

Avoiding drying-artifacts in transmission electron microscopy: Characterizing the size and colloidal state of nanoparticles
por: Michen, Benjamin, et al.
Publicado: (2015)

Cellular Uptake of Silica and Gold Nanoparticles Induces Early Activation of Nuclear Receptor NR4A1
por: Sousa de Almeida, Mauro, et al.
Publicado: (2022)

Assessing the Stability of Fluorescently Encoded Nanoparticles in Lysosomes by Using Complementary Methods
por: Milosevic, Ana M., et al.
Publicado: (2017)

Detection of Sub-Micro- and Nanoplastic Particles on Gold Nanoparticle-Based Substrates through Surface-Enhanced Raman Scattering (SERS) Spectroscopy
por: Caldwell, Jessica, et al.
Publicado: (2021)

A comparative study of silver nanoparticle dissolution under physiological conditions
por: Steinmetz, Lukas, et al.
Publicado: (2020)

Substrate stiffness reduces particle uptake by epithelial cells and macrophages in a size-dependent manner through mechanoregulation
por: Lee, Aaron, et al.
Publicado: (2022)

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages
por: Kuhn, Dagmar A, et al.
Publicado: (2014)

Biodistribution of single and aggregated gold nanoparticles exposed to the human lung epithelial tissue barrier at the air-liquid interface
por: Durantie, Estelle, et al.
Publicado: (2017)

Designing the ultrasonic treatment of nanoparticle-dispersions via machine learning
por: Glaubitz, Christina, et al.
Publicado: (2022)

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages
por: Vanhecke, Dimitri, et al.
Publicado: (2017)

Size-dependent accumulation of particles in lysosomes modulates dendritic cell function through impaired antigen degradation
por: Seydoux, Emilie, et al.
Publicado: (2014)

Cellular Uptake of Silica Particles Influences EGFR Signaling Pathway and is Affected in Response to EGF
por: Sousa de Almeida, Mauro, et al.
Publicado: (2023)

A novel technique to determine the cell type specific response within an in vitro co-culture model via multi-colour flow cytometry
por: Clift, Martin J. D., et al.
Publicado: (2017)

A Near-Infrared Mechanically Switchable Elastomeric Film as a Dynamic Cell Culture Substrate
por: Spiaggia, Giovanni, et al.
Publicado: (2022)

Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy
por: Septiadi, Dedy, et al.
Publicado: (2019)

Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing
por: Hauser, Daniel, et al.
Publicado: (2018)

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches
por: Herzog, Fabian, et al.
Publicado: (2014)

Correlative Light, Electron Microscopy and Raman Spectroscopy Workflow To Detect and Observe Microplastic Interactions with Whole Jellyfish
por: Caldwell, Jessica, et al.
Publicado: (2023)

Ultrathin Ceramic Membranes as Scaffolds for Functional Cell Coculture Models on a Biomimetic Scale
por: Jud, Corinne, et al.
Publicado: (2015)

Diesel exhaust: current knowledge of adverse effects and underlying cellular mechanisms
por: Steiner, Sandro, et al.
Publicado: (2016)

An in vitro testing strategy towards mimicking the inhalation of high aspect ratio nanoparticles
por: Endes, Carola, et al.
Publicado: (2014)

Engineered nanomaterials: toward effective safety management in research laboratories
por: Groso, Amela, et al.
Publicado: (2016)

From Bioinspired Glue to Medicine: Polydopamine as a Biomedical Material
por: Hauser, Daniel, et al.
Publicado: (2020)

Nanoparticle administration method in cell culture alters particle-cell interaction
por: Moore, Thomas L., et al.
Publicado: (2019)

Impurities in polyvinylpyrrolidone: the key factor in the synthesis of gold nanostars
por: Taladriz-Blanco, Patricia, et al.
Publicado: (2021)

A biological perspective toward the interaction of theranostic nanoparticles with the bloodstream – what needs to be considered?
por: Clift, Martin J. D., et al.
Publicado: (2015)

Engineering an in vitro air-blood barrier by 3D bioprinting
por: Horváth, Lenke, et al.
Publicado: (2015)

An In Vitro Lung System to Assess the Proinflammatory Hazard of Carbon Nanotube Aerosols
por: Barosova, Hana, et al.
Publicado: (2020)

Encapsulated salts in velvet worm slime drive its hardening
por: Corrales-Ureña, Yendry Regina, et al.
Publicado: (2022)

Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract
por: Kuhn, Dagmar A, et al.
Publicado: (2015)

Assessment of a panel of interleukin-8 reporter lung epithelial cell lines to monitor the pro-inflammatory response following zinc oxide nanoparticle exposure under different cell culture conditions
por: Stoehr, Linda C., et al.
Publicado: (2015)

Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at the air-liquid interface
por: Herzog, Fabian, et al.
Publicado: (2013)

Interaction of biomedical nanoparticles with the pulmonary immune system
por: Blank, Fabian, et al.
Publicado: (2017)

Biological response of an in vitro human 3D lung cell model exposed to brake wear debris varies based on brake pad formulation
por: Barosova, Hana, et al.
Publicado: (2018)

Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies
por: Dijkhoff, Irini M., et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_v9luv60qtp00g9q7mtt90s4lg3