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DNA methylation alternation in Stanford- A acute aortic dissection

BACKGROUND: Acute aortic dissection (AAD) is a life-threatening cardiovascular disease. Recent studies have shown that DNA methylation may be associated with the pathological mechanism of AAD, but the panorama of DNA methylation needs to be explored. METHODS: DNA methylation patterns were screened u...

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Autores principales: Chen, Yufei, Xu, Xu, Chen, Zhaoran, Huang, Bi, Wang, Xiaojian, Fan, Xiaohan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618190/
https://www.ncbi.nlm.nih.gov/pubmed/36309656
http://dx.doi.org/10.1186/s12872-022-02882-5
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author Chen, Yufei
Xu, Xu
Chen, Zhaoran
Huang, Bi
Wang, Xiaojian
Fan, Xiaohan
author_facet Chen, Yufei
Xu, Xu
Chen, Zhaoran
Huang, Bi
Wang, Xiaojian
Fan, Xiaohan
author_sort Chen, Yufei
collection PubMed
description BACKGROUND: Acute aortic dissection (AAD) is a life-threatening cardiovascular disease. Recent studies have shown that DNA methylation may be associated with the pathological mechanism of AAD, but the panorama of DNA methylation needs to be explored. METHODS: DNA methylation patterns were screened using Infinium Human Methylation 450 K BeadChip in the aortic tissues from 4 patients with Stanford-A AAD and 4 controls. Gene enrichment was analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene ontology (GO). DNA methylation levels of candidate genes were determined by pyrosequencing in the replication cohort including 16 patients with AAD and 7 controls. Protein expression level of candidate gene was assessed by Western blot. RESULTS: A total of 589 differentially methylated positions including 315 hypomethylated and 274 hypermethylated positions were found in AAD group. KEGG analysis demonstrated that differentially methylated position-associated genes were enriched in MAPK signaling pathway, TNF signaling pathway and apoptosis pathway, et al. GO analysis demonstrated that differentially methylated position-associated genes were enriched in protein binding, angiogenesis and heart development et al. The differential DNA methylation in five key genes, including Fas, ANGPT2, DUSP6, FARP1 and CARD6, was authenticated in the independent replication cohort. The protein expression level of the Fas was increased by 1.78 times, indicating the possible role of DNA methylation in regulation of gene expression. CONCLUSION: DNA methylation was markedly changed in the aortic tissues of Stanford-A AAD and associated with gene dysregulation, involved in AAD progression. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at10.1186/s12872-022-02882-5.
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spelling pubmed-96181902022-10-31 DNA methylation alternation in Stanford- A acute aortic dissection Chen, Yufei Xu, Xu Chen, Zhaoran Huang, Bi Wang, Xiaojian Fan, Xiaohan BMC Cardiovasc Disord Research BACKGROUND: Acute aortic dissection (AAD) is a life-threatening cardiovascular disease. Recent studies have shown that DNA methylation may be associated with the pathological mechanism of AAD, but the panorama of DNA methylation needs to be explored. METHODS: DNA methylation patterns were screened using Infinium Human Methylation 450 K BeadChip in the aortic tissues from 4 patients with Stanford-A AAD and 4 controls. Gene enrichment was analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and gene ontology (GO). DNA methylation levels of candidate genes were determined by pyrosequencing in the replication cohort including 16 patients with AAD and 7 controls. Protein expression level of candidate gene was assessed by Western blot. RESULTS: A total of 589 differentially methylated positions including 315 hypomethylated and 274 hypermethylated positions were found in AAD group. KEGG analysis demonstrated that differentially methylated position-associated genes were enriched in MAPK signaling pathway, TNF signaling pathway and apoptosis pathway, et al. GO analysis demonstrated that differentially methylated position-associated genes were enriched in protein binding, angiogenesis and heart development et al. The differential DNA methylation in five key genes, including Fas, ANGPT2, DUSP6, FARP1 and CARD6, was authenticated in the independent replication cohort. The protein expression level of the Fas was increased by 1.78 times, indicating the possible role of DNA methylation in regulation of gene expression. CONCLUSION: DNA methylation was markedly changed in the aortic tissues of Stanford-A AAD and associated with gene dysregulation, involved in AAD progression. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at10.1186/s12872-022-02882-5. BioMed Central 2022-10-29 /pmc/articles/PMC9618190/ /pubmed/36309656 http://dx.doi.org/10.1186/s12872-022-02882-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Chen, Yufei
Xu, Xu
Chen, Zhaoran
Huang, Bi
Wang, Xiaojian
Fan, Xiaohan
DNA methylation alternation in Stanford- A acute aortic dissection
title DNA methylation alternation in Stanford- A acute aortic dissection
title_full DNA methylation alternation in Stanford- A acute aortic dissection
title_fullStr DNA methylation alternation in Stanford- A acute aortic dissection
title_full_unstemmed DNA methylation alternation in Stanford- A acute aortic dissection
title_short DNA methylation alternation in Stanford- A acute aortic dissection
title_sort dna methylation alternation in stanford- a acute aortic dissection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618190/
https://www.ncbi.nlm.nih.gov/pubmed/36309656
http://dx.doi.org/10.1186/s12872-022-02882-5
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