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Vimentin is an important ACE2 co-receptor for SARS-CoV-2 in epithelial cells

Vimentin is a type III intermediate filament protein, widely expressed in mesenchymal cells. Mainly located in the cytoplasm, vimentin can also appear at extracellular locations, where it may interact with bacterial or viral pathogens. In this study, we aimed at investigating the implication of vime...

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Autores principales: Arrindell, Jeffrey, Abou Atmeh, Perla, Jayet, Laurie, Sereme, Youssouf, Mege, Jean-Louis, Desnues, Benoit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618295/
https://www.ncbi.nlm.nih.gov/pubmed/36338433
http://dx.doi.org/10.1016/j.isci.2022.105463
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author Arrindell, Jeffrey
Abou Atmeh, Perla
Jayet, Laurie
Sereme, Youssouf
Mege, Jean-Louis
Desnues, Benoit
author_facet Arrindell, Jeffrey
Abou Atmeh, Perla
Jayet, Laurie
Sereme, Youssouf
Mege, Jean-Louis
Desnues, Benoit
author_sort Arrindell, Jeffrey
collection PubMed
description Vimentin is a type III intermediate filament protein, widely expressed in mesenchymal cells. Mainly located in the cytoplasm, vimentin can also appear at extracellular locations, where it may interact with bacterial or viral pathogens. In this study, we aimed at investigating the implication of vimentin in SARS-CoV-2 viral entry and the consequences on viral replication and cellular response. We showed that upon infection, vimentin was upregulated at the cell surface, where it interacts with ACE2 for SARS-CoV-2 entry. We demonstrated a direct interaction between SARS-CoV-2 spike protein, ACE2, and vimentin in epithelial cells. Inhibition of cell-surface vimentin availability resulted in reduced viral entry and cytopathogenic effects. Finally, we showed that the expression of inflammatory cytokines and chemokines was modulated by vimentin—SARS-CoV-2 interaction. In conclusion, our data suggest that cell-surface vimentin acts as a co-receptor for SARS-CoV-2.
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spelling pubmed-96182952022-10-31 Vimentin is an important ACE2 co-receptor for SARS-CoV-2 in epithelial cells Arrindell, Jeffrey Abou Atmeh, Perla Jayet, Laurie Sereme, Youssouf Mege, Jean-Louis Desnues, Benoit iScience Article Vimentin is a type III intermediate filament protein, widely expressed in mesenchymal cells. Mainly located in the cytoplasm, vimentin can also appear at extracellular locations, where it may interact with bacterial or viral pathogens. In this study, we aimed at investigating the implication of vimentin in SARS-CoV-2 viral entry and the consequences on viral replication and cellular response. We showed that upon infection, vimentin was upregulated at the cell surface, where it interacts with ACE2 for SARS-CoV-2 entry. We demonstrated a direct interaction between SARS-CoV-2 spike protein, ACE2, and vimentin in epithelial cells. Inhibition of cell-surface vimentin availability resulted in reduced viral entry and cytopathogenic effects. Finally, we showed that the expression of inflammatory cytokines and chemokines was modulated by vimentin—SARS-CoV-2 interaction. In conclusion, our data suggest that cell-surface vimentin acts as a co-receptor for SARS-CoV-2. Elsevier 2022-10-30 /pmc/articles/PMC9618295/ /pubmed/36338433 http://dx.doi.org/10.1016/j.isci.2022.105463 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Arrindell, Jeffrey
Abou Atmeh, Perla
Jayet, Laurie
Sereme, Youssouf
Mege, Jean-Louis
Desnues, Benoit
Vimentin is an important ACE2 co-receptor for SARS-CoV-2 in epithelial cells
title Vimentin is an important ACE2 co-receptor for SARS-CoV-2 in epithelial cells
title_full Vimentin is an important ACE2 co-receptor for SARS-CoV-2 in epithelial cells
title_fullStr Vimentin is an important ACE2 co-receptor for SARS-CoV-2 in epithelial cells
title_full_unstemmed Vimentin is an important ACE2 co-receptor for SARS-CoV-2 in epithelial cells
title_short Vimentin is an important ACE2 co-receptor for SARS-CoV-2 in epithelial cells
title_sort vimentin is an important ace2 co-receptor for sars-cov-2 in epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618295/
https://www.ncbi.nlm.nih.gov/pubmed/36338433
http://dx.doi.org/10.1016/j.isci.2022.105463
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