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Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites

Endometritis is persistent inflammation caused by bacteria, which can lead to infertility. Although traditional Chinese medicine (TCM) has been used to treat endometritis, the underlying mechanism is still unclear. Here, Baogong Decoction (BGD), a TCM compound, was used to treat mouse endometritis i...

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Detalles Bibliográficos
Autores principales: Ding, He, Wang, Youyuan, Li, Zhiqiang, Li, Qianqing, Liu, Hongyu, Zhao, Jing, Lu, Wenfa, Wang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618315/
https://www.ncbi.nlm.nih.gov/pubmed/35932174
http://dx.doi.org/10.1111/1751-7915.14127
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author Ding, He
Wang, Youyuan
Li, Zhiqiang
Li, Qianqing
Liu, Hongyu
Zhao, Jing
Lu, Wenfa
Wang, Jun
author_facet Ding, He
Wang, Youyuan
Li, Zhiqiang
Li, Qianqing
Liu, Hongyu
Zhao, Jing
Lu, Wenfa
Wang, Jun
author_sort Ding, He
collection PubMed
description Endometritis is persistent inflammation caused by bacteria, which can lead to infertility. Although traditional Chinese medicine (TCM) has been used to treat endometritis, the underlying mechanism is still unclear. Here, Baogong Decoction (BGD), a TCM compound, was used to treat mouse endometritis induced by Escherichia coli (E. coli), and then 16S rRNA sequencing and non‐targeted metabolomics were used to investigate the change of uterine microbiota and metabolomes in serum and uterine after BGD treatment. Finally, the therapeutic effect of potential metabolites for treating mouse endometritis screened by combined omics analyses was verified using pathological model. The results showed that BGD treatment could effectively treat endometritis associated with the increasing relative abundance of Firmicutes, Bacteroides, Lactobacillus and Lactococcus, and the decreasing relative abundance of Cupriavidus and Proteobacteria. 133 and 130 metabolites were found to be potential biomarkers in serum and uterine tissue respectively. In serum and tissues, dehydroepiandrosterone (DHEA) and catechol were significantly increased in the BGD treatment versus the inflammation group. Results of combined omics analyses demonstrated that DHEA was positively correlated with changes in microbiota. Results of pathological model demonstrated that DHEA could cure endometritis effectively associated with the decreasing infiltration of inflammatory cells and expression of inflammatory factors in the uterus. In summary, our results demonstrated that BGD could cure endometritis in mice by modulating the structure of the uterine microbiota and its metabolites, in which DHEA may be one of the main components of the therapeutic effect of BGD.
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spelling pubmed-96183152022-11-01 Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites Ding, He Wang, Youyuan Li, Zhiqiang Li, Qianqing Liu, Hongyu Zhao, Jing Lu, Wenfa Wang, Jun Microb Biotechnol Research Articles Endometritis is persistent inflammation caused by bacteria, which can lead to infertility. Although traditional Chinese medicine (TCM) has been used to treat endometritis, the underlying mechanism is still unclear. Here, Baogong Decoction (BGD), a TCM compound, was used to treat mouse endometritis induced by Escherichia coli (E. coli), and then 16S rRNA sequencing and non‐targeted metabolomics were used to investigate the change of uterine microbiota and metabolomes in serum and uterine after BGD treatment. Finally, the therapeutic effect of potential metabolites for treating mouse endometritis screened by combined omics analyses was verified using pathological model. The results showed that BGD treatment could effectively treat endometritis associated with the increasing relative abundance of Firmicutes, Bacteroides, Lactobacillus and Lactococcus, and the decreasing relative abundance of Cupriavidus and Proteobacteria. 133 and 130 metabolites were found to be potential biomarkers in serum and uterine tissue respectively. In serum and tissues, dehydroepiandrosterone (DHEA) and catechol were significantly increased in the BGD treatment versus the inflammation group. Results of combined omics analyses demonstrated that DHEA was positively correlated with changes in microbiota. Results of pathological model demonstrated that DHEA could cure endometritis effectively associated with the decreasing infiltration of inflammatory cells and expression of inflammatory factors in the uterus. In summary, our results demonstrated that BGD could cure endometritis in mice by modulating the structure of the uterine microbiota and its metabolites, in which DHEA may be one of the main components of the therapeutic effect of BGD. John Wiley and Sons Inc. 2022-08-06 /pmc/articles/PMC9618315/ /pubmed/35932174 http://dx.doi.org/10.1111/1751-7915.14127 Text en © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Ding, He
Wang, Youyuan
Li, Zhiqiang
Li, Qianqing
Liu, Hongyu
Zhao, Jing
Lu, Wenfa
Wang, Jun
Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites
title Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites
title_full Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites
title_fullStr Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites
title_full_unstemmed Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites
title_short Baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites
title_sort baogong decoction treats endometritis in mice by regulating uterine microbiota structure and metabolites
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618315/
https://www.ncbi.nlm.nih.gov/pubmed/35932174
http://dx.doi.org/10.1111/1751-7915.14127
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