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Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation

Intravascular transplantation of human-induced pluripotent stem cells (hiPSCs) demonstrated a significant therapeutic effect in the treatment of restenosis by the paracrine function of extracellular vesicles (EVs). However, the risk of tumorigenicity and poor cell survival limits its clinical applic...

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Autores principales: He, Yecheng, Li, Quanfu, Feng, Feng, Gao, Rupan, Li, Huadong, Chu, Yuxin, Li, Shaobo, Wang, Yin, Mao, Ruoying, Ji, Zhongzhong, Hua, Yutao, Shen, Jun, Wang, Ziao, Zhao, Meng, Yao, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618599/
https://www.ncbi.nlm.nih.gov/pubmed/36324745
http://dx.doi.org/10.3389/fcvm.2022.922790
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author He, Yecheng
Li, Quanfu
Feng, Feng
Gao, Rupan
Li, Huadong
Chu, Yuxin
Li, Shaobo
Wang, Yin
Mao, Ruoying
Ji, Zhongzhong
Hua, Yutao
Shen, Jun
Wang, Ziao
Zhao, Meng
Yao, Qing
author_facet He, Yecheng
Li, Quanfu
Feng, Feng
Gao, Rupan
Li, Huadong
Chu, Yuxin
Li, Shaobo
Wang, Yin
Mao, Ruoying
Ji, Zhongzhong
Hua, Yutao
Shen, Jun
Wang, Ziao
Zhao, Meng
Yao, Qing
author_sort He, Yecheng
collection PubMed
description Intravascular transplantation of human-induced pluripotent stem cells (hiPSCs) demonstrated a significant therapeutic effect in the treatment of restenosis by the paracrine function of extracellular vesicles (EVs). However, the risk of tumorigenicity and poor cell survival limits its clinical applications. In this study, we for the first time applied a highly efficient and robust three-dimensional (3D) protocol for hiPSC differentiation into endothelial cells (ECs) with subsequent isolation of EVs from the derived hiPSC-EC (ECs differentiated from hiPSCs), and validated their therapeutic effect in intimal hyperplasia (IH) models. We found that intravenously (iv) injected EVs could accumulate on the carotid artery endothelium and significantly alleviate the intimal thickening induced by the carotid artery ligation. To elucidate the mechanism of this endothelial protection, we performed miRNA expression profiling and found out that among the most conserved endothelial miRNAs, miR-126 was the most abundant in hiPSC-EC-produced EVs (hiPSC-EC-EV). MiR-126 depletion from hiPSC-EC-EV can hinder its protective effect on human umbilical vein endothelial cells (HUVECs) in an inflammatory process. A variety of functional in vitro studies revealed that miR-126 was able to prevent endothelial apoptosis after inflammatory stimulation, as well as promote EC migration and tube formation through autophagy upregulation. The latter was supported by in vivo studies demonstrating that treatment with hiPSC-EC-EV can upregulate autophagy in mouse carotid artery ECs, thereby preventing IH and modulating vascular homeostasis via remodeling of the vascular intima. Our findings suggest a regulatory mechanism for the therapeutic effect on arterial restenosis by autophagy regulation, and provide a potential strategy for clinical treatment of the disease.
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spelling pubmed-96185992022-11-01 Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation He, Yecheng Li, Quanfu Feng, Feng Gao, Rupan Li, Huadong Chu, Yuxin Li, Shaobo Wang, Yin Mao, Ruoying Ji, Zhongzhong Hua, Yutao Shen, Jun Wang, Ziao Zhao, Meng Yao, Qing Front Cardiovasc Med Cardiovascular Medicine Intravascular transplantation of human-induced pluripotent stem cells (hiPSCs) demonstrated a significant therapeutic effect in the treatment of restenosis by the paracrine function of extracellular vesicles (EVs). However, the risk of tumorigenicity and poor cell survival limits its clinical applications. In this study, we for the first time applied a highly efficient and robust three-dimensional (3D) protocol for hiPSC differentiation into endothelial cells (ECs) with subsequent isolation of EVs from the derived hiPSC-EC (ECs differentiated from hiPSCs), and validated their therapeutic effect in intimal hyperplasia (IH) models. We found that intravenously (iv) injected EVs could accumulate on the carotid artery endothelium and significantly alleviate the intimal thickening induced by the carotid artery ligation. To elucidate the mechanism of this endothelial protection, we performed miRNA expression profiling and found out that among the most conserved endothelial miRNAs, miR-126 was the most abundant in hiPSC-EC-produced EVs (hiPSC-EC-EV). MiR-126 depletion from hiPSC-EC-EV can hinder its protective effect on human umbilical vein endothelial cells (HUVECs) in an inflammatory process. A variety of functional in vitro studies revealed that miR-126 was able to prevent endothelial apoptosis after inflammatory stimulation, as well as promote EC migration and tube formation through autophagy upregulation. The latter was supported by in vivo studies demonstrating that treatment with hiPSC-EC-EV can upregulate autophagy in mouse carotid artery ECs, thereby preventing IH and modulating vascular homeostasis via remodeling of the vascular intima. Our findings suggest a regulatory mechanism for the therapeutic effect on arterial restenosis by autophagy regulation, and provide a potential strategy for clinical treatment of the disease. Frontiers Media S.A. 2022-10-17 /pmc/articles/PMC9618599/ /pubmed/36324745 http://dx.doi.org/10.3389/fcvm.2022.922790 Text en Copyright © 2022 He, Li, Feng, Gao, Li, Chu, Li, Wang, Mao, Ji, Hua, Shen, Wang, Zhao and Yao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
He, Yecheng
Li, Quanfu
Feng, Feng
Gao, Rupan
Li, Huadong
Chu, Yuxin
Li, Shaobo
Wang, Yin
Mao, Ruoying
Ji, Zhongzhong
Hua, Yutao
Shen, Jun
Wang, Ziao
Zhao, Meng
Yao, Qing
Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation
title Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation
title_full Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation
title_fullStr Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation
title_full_unstemmed Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation
title_short Extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation
title_sort extracellular vesicles produced by human-induced pluripotent stem cell-derived endothelial cells can prevent arterial stenosis in mice via autophagy regulation
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9618599/
https://www.ncbi.nlm.nih.gov/pubmed/36324745
http://dx.doi.org/10.3389/fcvm.2022.922790
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