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Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes

Osteoarthritis (OA) reduces the quality of life as a result of the pain caused by continuous joint destruction. Inactivated Lactobacillus (LA-1) ameliorated osteoarthritis and protected cartilage by modulating inflammation. In this study, we evaluated the mechanism by which live LA-1 ameliorated OA....

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Autores principales: Cho, Keun-Hyung, Na, Hyun Sik, Jhun, JooYeon, Woo, Jin Seok, Lee, A Ram, Lee, Seung Yoon, Lee, Jeong Su, Um, In Gyu, Kim, Seok Jung, Park, Sung-Hwan, Cho, Mi-La
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619036/
https://www.ncbi.nlm.nih.gov/pubmed/36325344
http://dx.doi.org/10.3389/fimmu.2022.930511
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author Cho, Keun-Hyung
Na, Hyun Sik
Jhun, JooYeon
Woo, Jin Seok
Lee, A Ram
Lee, Seung Yoon
Lee, Jeong Su
Um, In Gyu
Kim, Seok Jung
Park, Sung-Hwan
Cho, Mi-La
author_facet Cho, Keun-Hyung
Na, Hyun Sik
Jhun, JooYeon
Woo, Jin Seok
Lee, A Ram
Lee, Seung Yoon
Lee, Jeong Su
Um, In Gyu
Kim, Seok Jung
Park, Sung-Hwan
Cho, Mi-La
author_sort Cho, Keun-Hyung
collection PubMed
description Osteoarthritis (OA) reduces the quality of life as a result of the pain caused by continuous joint destruction. Inactivated Lactobacillus (LA-1) ameliorated osteoarthritis and protected cartilage by modulating inflammation. In this study, we evaluated the mechanism by which live LA-1 ameliorated OA. To investigate the effect of live LA-1 on OA progression, we administered LA-1 into monosodium iodoacetate (MIA)-induced OA animals. The pain threshold, cartilage damage, and inflammation of the joint synovial membrane were improved by live LA-1. Furthermore, the analysis of intestinal tissues and feces in the disease model has been shown to affect the systems of the intestinal system and improve the microbiome environment. Interestingly, inflammation of the intestinal tissue was reduced, and the intestinal microbiome was altered by live LA-1. Live LA-1 administration led to an increase in the level of Faecalibacterium which is a short-chain fatty acid (SCFA) butyrate-producing bacteria. The daily supply of butyrate, a bacterial SCFA, showed a tendency to decrease necroptosis, a type of abnormal cell death, by inducing autophagy and reversing impaired autophagy by the inflammatory environment. These results suggest that OA is modulated by changes in the gut microbiome, suggesting that activation of autophagy can reduce aberrant cell death. In summary, live LA-1 or butyrate ameliorates OA progression by modulating the gut environment and autophagic flux. Our findings suggest the regulation of the gut microenvironment as a therapeutic target for OA.
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spelling pubmed-96190362022-11-01 Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes Cho, Keun-Hyung Na, Hyun Sik Jhun, JooYeon Woo, Jin Seok Lee, A Ram Lee, Seung Yoon Lee, Jeong Su Um, In Gyu Kim, Seok Jung Park, Sung-Hwan Cho, Mi-La Front Immunol Immunology Osteoarthritis (OA) reduces the quality of life as a result of the pain caused by continuous joint destruction. Inactivated Lactobacillus (LA-1) ameliorated osteoarthritis and protected cartilage by modulating inflammation. In this study, we evaluated the mechanism by which live LA-1 ameliorated OA. To investigate the effect of live LA-1 on OA progression, we administered LA-1 into monosodium iodoacetate (MIA)-induced OA animals. The pain threshold, cartilage damage, and inflammation of the joint synovial membrane were improved by live LA-1. Furthermore, the analysis of intestinal tissues and feces in the disease model has been shown to affect the systems of the intestinal system and improve the microbiome environment. Interestingly, inflammation of the intestinal tissue was reduced, and the intestinal microbiome was altered by live LA-1. Live LA-1 administration led to an increase in the level of Faecalibacterium which is a short-chain fatty acid (SCFA) butyrate-producing bacteria. The daily supply of butyrate, a bacterial SCFA, showed a tendency to decrease necroptosis, a type of abnormal cell death, by inducing autophagy and reversing impaired autophagy by the inflammatory environment. These results suggest that OA is modulated by changes in the gut microbiome, suggesting that activation of autophagy can reduce aberrant cell death. In summary, live LA-1 or butyrate ameliorates OA progression by modulating the gut environment and autophagic flux. Our findings suggest the regulation of the gut microenvironment as a therapeutic target for OA. Frontiers Media S.A. 2022-10-17 /pmc/articles/PMC9619036/ /pubmed/36325344 http://dx.doi.org/10.3389/fimmu.2022.930511 Text en Copyright © 2022 Cho, Na, Jhun, Woo, Lee, Lee, Lee, Um, Kim, Park and Cho https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Cho, Keun-Hyung
Na, Hyun Sik
Jhun, JooYeon
Woo, Jin Seok
Lee, A Ram
Lee, Seung Yoon
Lee, Jeong Su
Um, In Gyu
Kim, Seok Jung
Park, Sung-Hwan
Cho, Mi-La
Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes
title Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes
title_full Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes
title_fullStr Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes
title_full_unstemmed Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes
title_short Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes
title_sort lactobacillus (la-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619036/
https://www.ncbi.nlm.nih.gov/pubmed/36325344
http://dx.doi.org/10.3389/fimmu.2022.930511
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