The His-tag as a decoy modulating preferred orientation in cryoEM

The His-tag is a widely used affinity tag that facilitates purification by means of affinity chromatography of recombinant proteins for functional and structural studies. We show here that His-tag presence affects how coproheme decarboxylase interacts with the air-water interface during grid prepara...

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Detalles Bibliográficos
Autores principales: Bromberg, Raquel, Cai, Kai, Guo, Yirui, Plymire, Daniel, Emde, Tabitha, Puzio, Maciej, Borek, Dominika, Otwinowski, Zbyszek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619061/
https://www.ncbi.nlm.nih.gov/pubmed/36325274
http://dx.doi.org/10.3389/fmolb.2022.912072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619061/
https://www.ncbi.nlm.nih.gov/pubmed/36325274
http://dx.doi.org/10.3389/fmolb.2022.912072

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