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The His-tag as a decoy modulating preferred orientation in cryoEM
The His-tag is a widely used affinity tag that facilitates purification by means of affinity chromatography of recombinant proteins for functional and structural studies. We show here that His-tag presence affects how coproheme decarboxylase interacts with the air-water interface during grid prepara...
Autores principales: | Bromberg, Raquel, Cai, Kai, Guo, Yirui, Plymire, Daniel, Emde, Tabitha, Puzio, Maciej, Borek, Dominika, Otwinowski, Zbyszek |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619061/ https://www.ncbi.nlm.nih.gov/pubmed/36325274 http://dx.doi.org/10.3389/fmolb.2022.912072 |
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