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Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer
BACKGROUND: Colon cancer is common worldwide, with high morbidity and poor prognosis. Ferroptosis is a novel form of cell death driven by the accumulation of iron-dependent lipid peroxides, which differs from other programmed cell death mechanisms. Programmed cell death is a cancer hallmark, and fer...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619366/ https://www.ncbi.nlm.nih.gov/pubmed/36324584 http://dx.doi.org/10.3389/fonc.2022.1033044 |
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author | Huang, Hongliang Dai, Yuexiang Duan, Yingying Yuan, Zhongwen Li, Yanxuan Zhang, Maomao Zhu, Wenting Yu, Hang Zhong, Wenfei Feng, Senling |
author_facet | Huang, Hongliang Dai, Yuexiang Duan, Yingying Yuan, Zhongwen Li, Yanxuan Zhang, Maomao Zhu, Wenting Yu, Hang Zhong, Wenfei Feng, Senling |
author_sort | Huang, Hongliang |
collection | PubMed |
description | BACKGROUND: Colon cancer is common worldwide, with high morbidity and poor prognosis. Ferroptosis is a novel form of cell death driven by the accumulation of iron-dependent lipid peroxides, which differs from other programmed cell death mechanisms. Programmed cell death is a cancer hallmark, and ferroptosis is known to participate in various cancers, including colon cancer. Novel ferroptosis markers and targeted colon cancer therapies are urgently needed. To this end, we performed a preliminary exploration of ferroptosis-related genes in colon cancer to enable new treatment strategies. METHODS: Ferroptosis-related genes in colon cancer were obtained by data mining and screening for differentially expressed genes (DEGs) using bioinformatics analysis tools. We normalized the data across four independent datasets and a ferroptosis-specific database. Identified genes were validated by immunohistochemical analysis of pathological and healthy clinical samples. RESULTS: We identified DEGs in colon cancer that are involved in ferroptosis. Among these, five core genes were found: ELAVL1, GPX2, EPAS1, SLC7A5, and HMGB1. Bioinformatics analyses revealed that the expression of all five genes, except for EPAS1, was higher in tumor tissues than in healthy tissues. CONCLUSIONS: The preliminary exploration of the five core genes revealed that they are differentially expressed in colon cancer, playing an essential role in ferroptosis. This study provides a foundation for subsequent research on ferroptosis in colon cancer. |
format | Online Article Text |
id | pubmed-9619366 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96193662022-11-01 Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer Huang, Hongliang Dai, Yuexiang Duan, Yingying Yuan, Zhongwen Li, Yanxuan Zhang, Maomao Zhu, Wenting Yu, Hang Zhong, Wenfei Feng, Senling Front Oncol Oncology BACKGROUND: Colon cancer is common worldwide, with high morbidity and poor prognosis. Ferroptosis is a novel form of cell death driven by the accumulation of iron-dependent lipid peroxides, which differs from other programmed cell death mechanisms. Programmed cell death is a cancer hallmark, and ferroptosis is known to participate in various cancers, including colon cancer. Novel ferroptosis markers and targeted colon cancer therapies are urgently needed. To this end, we performed a preliminary exploration of ferroptosis-related genes in colon cancer to enable new treatment strategies. METHODS: Ferroptosis-related genes in colon cancer were obtained by data mining and screening for differentially expressed genes (DEGs) using bioinformatics analysis tools. We normalized the data across four independent datasets and a ferroptosis-specific database. Identified genes were validated by immunohistochemical analysis of pathological and healthy clinical samples. RESULTS: We identified DEGs in colon cancer that are involved in ferroptosis. Among these, five core genes were found: ELAVL1, GPX2, EPAS1, SLC7A5, and HMGB1. Bioinformatics analyses revealed that the expression of all five genes, except for EPAS1, was higher in tumor tissues than in healthy tissues. CONCLUSIONS: The preliminary exploration of the five core genes revealed that they are differentially expressed in colon cancer, playing an essential role in ferroptosis. This study provides a foundation for subsequent research on ferroptosis in colon cancer. Frontiers Media S.A. 2022-10-17 /pmc/articles/PMC9619366/ /pubmed/36324584 http://dx.doi.org/10.3389/fonc.2022.1033044 Text en Copyright © 2022 Huang, Dai, Duan, Yuan, Li, Zhang, Zhu, Yu, Zhong and Feng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Huang, Hongliang Dai, Yuexiang Duan, Yingying Yuan, Zhongwen Li, Yanxuan Zhang, Maomao Zhu, Wenting Yu, Hang Zhong, Wenfei Feng, Senling Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer |
title | Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer |
title_full | Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer |
title_fullStr | Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer |
title_full_unstemmed | Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer |
title_short | Effective prediction of potential ferroptosis critical genes in clinical colorectal cancer |
title_sort | effective prediction of potential ferroptosis critical genes in clinical colorectal cancer |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619366/ https://www.ncbi.nlm.nih.gov/pubmed/36324584 http://dx.doi.org/10.3389/fonc.2022.1033044 |
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