A New Auto-RPA-Fluorescence Detection Platform for SARS-CoV-2

OBJECTIVE: The outbreak of COVID-19 caused by SARS-CoV-2 has led to a serious worldwide pandemic. Quantitative reverse transcription–polymerase chain reaction (qRT-PCR)–based methods were recommended for routine detection of SARS-CoV-2 RNA. Because the reaction time and analytical sensitivity of qRT...

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Detalles Bibliográficos
Autores principales: Tian, Jing, Chen, Biao, Zhang, Bin, Li, Tantan, Liang, Zhiqiang, Guo, Yujin, Jiao, Huping, Liang, Fenghong, Xiang, Longquan, Lin, Fanzhong, Ren, Ruiwen, Liu, Qingbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619631/
https://www.ncbi.nlm.nih.gov/pubmed/36200614
http://dx.doi.org/10.1093/labmed/lmac093
Descripción
Sumario:OBJECTIVE: The outbreak of COVID-19 caused by SARS-CoV-2 has led to a serious worldwide pandemic. Quantitative reverse transcription–polymerase chain reaction (qRT-PCR)–based methods were recommended for routine detection of SARS-CoV-2 RNA. Because the reaction time and analytical sensitivity of qRT-PCR limits the diagnosis of SARS-CoV-2, development of a quick process of SARS-CoV-2 detection technology with high analytical sensitivity remains urgent. METHODS: We combined isothermal amplification and fluorescence detection technology to develop a new auto-recombinase polymerase amplification (RPA)-fluorescence platform that could be used in the diagnosis of SARS-CoV-2. RESULTS: By optimization of primers and probes, the RPA platform could detect SARS-CoV-2 nucleotides within 15 min. The limits of detection and specificity of the auto-RPA-fluorescence platform were 5 copies/µL and 100%, respectively. The accuracy of detection of the auto-RPA-fluorescence platform in the 16 positive samples was 100%. CONCLUSION: The RPA platform is a potential technology for the diagnosis of SARS-CoV-2 infection.

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