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Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection

BACKGROUND: The purpose of this study was to compare different invasive methods for Helicobacter pylori (H. pylori) detection, namely PCR for H. pylori specific ureC gene, Rapid urease test (RUT), and histopathological examination by modified Giemsa staining. METHODOLOGY: Endoscopic gastroduodenal b...

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Autores principales: Kismat, Saifa, Tanni, Nusrat Nur, Akhtar, Rokshana, Roy, Chandan Kumar, Rahman, Mohammad Mosiur, Molla, Md. Maruf Ahmed, Anwar, Shaheda, Ahmed, Sharmeen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619850/
https://www.ncbi.nlm.nih.gov/pubmed/36325107
http://dx.doi.org/10.1177/11786361221133947
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author Kismat, Saifa
Tanni, Nusrat Nur
Akhtar, Rokshana
Roy, Chandan Kumar
Rahman, Mohammad Mosiur
Molla, Md. Maruf Ahmed
Anwar, Shaheda
Ahmed, Sharmeen
author_facet Kismat, Saifa
Tanni, Nusrat Nur
Akhtar, Rokshana
Roy, Chandan Kumar
Rahman, Mohammad Mosiur
Molla, Md. Maruf Ahmed
Anwar, Shaheda
Ahmed, Sharmeen
author_sort Kismat, Saifa
collection PubMed
description BACKGROUND: The purpose of this study was to compare different invasive methods for Helicobacter pylori (H. pylori) detection, namely PCR for H. pylori specific ureC gene, Rapid urease test (RUT), and histopathological examination by modified Giemsa staining. METHODOLOGY: Endoscopic gastroduodenal biopsy materials were collected from dyspeptic patients who underwent endoscopic examination upon fulfilling the inclusion criteria. Three to four samples were collected from each patient after taking informed consent and proper clinical history. A rapid urease test (RUT) was done on spot with in-house RUT media from 1 specimen. One to two specimens were preserved in 10% formaldehyde for histopathology and PCR for ureC gene was done from 1 specimen. Collected biopsy specimens from gastric and duodenal mucosa of 142 patients were categorized as H. pylori-positive cases and H. pylori-negative cases based on the case definition used in the study upon positivity of 3 diagnostic tests. RESULTS: Among 142 biopsy specimens, 34.5% were categorized as H. pylori-positive cases, 35.2% as H. pylori-negative cases, and finally 30.2% as doubtful or indeterminate cases. Rapid urease test was the most sensitive method, closely followed by ureC gene PCR and histopathology, with a sensitivity of 94.2%, 83.0%, and 76.5%, respectively. Whereas histology was the most specific, having 98.0% specificity followed by 83.0% in PCR. RUT was the least specific, with 55.5% specificity. CONCLUSION: While histopathology could detect H. pylori infection with the highest specificity, for definitive diagnosis combination of any 2 methods should be used, if available.
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spelling pubmed-96198502022-11-01 Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection Kismat, Saifa Tanni, Nusrat Nur Akhtar, Rokshana Roy, Chandan Kumar Rahman, Mohammad Mosiur Molla, Md. Maruf Ahmed Anwar, Shaheda Ahmed, Sharmeen Microbiol Insights Brief Report BACKGROUND: The purpose of this study was to compare different invasive methods for Helicobacter pylori (H. pylori) detection, namely PCR for H. pylori specific ureC gene, Rapid urease test (RUT), and histopathological examination by modified Giemsa staining. METHODOLOGY: Endoscopic gastroduodenal biopsy materials were collected from dyspeptic patients who underwent endoscopic examination upon fulfilling the inclusion criteria. Three to four samples were collected from each patient after taking informed consent and proper clinical history. A rapid urease test (RUT) was done on spot with in-house RUT media from 1 specimen. One to two specimens were preserved in 10% formaldehyde for histopathology and PCR for ureC gene was done from 1 specimen. Collected biopsy specimens from gastric and duodenal mucosa of 142 patients were categorized as H. pylori-positive cases and H. pylori-negative cases based on the case definition used in the study upon positivity of 3 diagnostic tests. RESULTS: Among 142 biopsy specimens, 34.5% were categorized as H. pylori-positive cases, 35.2% as H. pylori-negative cases, and finally 30.2% as doubtful or indeterminate cases. Rapid urease test was the most sensitive method, closely followed by ureC gene PCR and histopathology, with a sensitivity of 94.2%, 83.0%, and 76.5%, respectively. Whereas histology was the most specific, having 98.0% specificity followed by 83.0% in PCR. RUT was the least specific, with 55.5% specificity. CONCLUSION: While histopathology could detect H. pylori infection with the highest specificity, for definitive diagnosis combination of any 2 methods should be used, if available. SAGE Publications 2022-10-28 /pmc/articles/PMC9619850/ /pubmed/36325107 http://dx.doi.org/10.1177/11786361221133947 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Brief Report
Kismat, Saifa
Tanni, Nusrat Nur
Akhtar, Rokshana
Roy, Chandan Kumar
Rahman, Mohammad Mosiur
Molla, Md. Maruf Ahmed
Anwar, Shaheda
Ahmed, Sharmeen
Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection
title Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection
title_full Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection
title_fullStr Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection
title_full_unstemmed Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection
title_short Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection
title_sort diagnosis and comparison of three invasive detection methods for helicobacter pylori infection
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619850/
https://www.ncbi.nlm.nih.gov/pubmed/36325107
http://dx.doi.org/10.1177/11786361221133947
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