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Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection
BACKGROUND: The purpose of this study was to compare different invasive methods for Helicobacter pylori (H. pylori) detection, namely PCR for H. pylori specific ureC gene, Rapid urease test (RUT), and histopathological examination by modified Giemsa staining. METHODOLOGY: Endoscopic gastroduodenal b...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619850/ https://www.ncbi.nlm.nih.gov/pubmed/36325107 http://dx.doi.org/10.1177/11786361221133947 |
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author | Kismat, Saifa Tanni, Nusrat Nur Akhtar, Rokshana Roy, Chandan Kumar Rahman, Mohammad Mosiur Molla, Md. Maruf Ahmed Anwar, Shaheda Ahmed, Sharmeen |
author_facet | Kismat, Saifa Tanni, Nusrat Nur Akhtar, Rokshana Roy, Chandan Kumar Rahman, Mohammad Mosiur Molla, Md. Maruf Ahmed Anwar, Shaheda Ahmed, Sharmeen |
author_sort | Kismat, Saifa |
collection | PubMed |
description | BACKGROUND: The purpose of this study was to compare different invasive methods for Helicobacter pylori (H. pylori) detection, namely PCR for H. pylori specific ureC gene, Rapid urease test (RUT), and histopathological examination by modified Giemsa staining. METHODOLOGY: Endoscopic gastroduodenal biopsy materials were collected from dyspeptic patients who underwent endoscopic examination upon fulfilling the inclusion criteria. Three to four samples were collected from each patient after taking informed consent and proper clinical history. A rapid urease test (RUT) was done on spot with in-house RUT media from 1 specimen. One to two specimens were preserved in 10% formaldehyde for histopathology and PCR for ureC gene was done from 1 specimen. Collected biopsy specimens from gastric and duodenal mucosa of 142 patients were categorized as H. pylori-positive cases and H. pylori-negative cases based on the case definition used in the study upon positivity of 3 diagnostic tests. RESULTS: Among 142 biopsy specimens, 34.5% were categorized as H. pylori-positive cases, 35.2% as H. pylori-negative cases, and finally 30.2% as doubtful or indeterminate cases. Rapid urease test was the most sensitive method, closely followed by ureC gene PCR and histopathology, with a sensitivity of 94.2%, 83.0%, and 76.5%, respectively. Whereas histology was the most specific, having 98.0% specificity followed by 83.0% in PCR. RUT was the least specific, with 55.5% specificity. CONCLUSION: While histopathology could detect H. pylori infection with the highest specificity, for definitive diagnosis combination of any 2 methods should be used, if available. |
format | Online Article Text |
id | pubmed-9619850 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-96198502022-11-01 Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection Kismat, Saifa Tanni, Nusrat Nur Akhtar, Rokshana Roy, Chandan Kumar Rahman, Mohammad Mosiur Molla, Md. Maruf Ahmed Anwar, Shaheda Ahmed, Sharmeen Microbiol Insights Brief Report BACKGROUND: The purpose of this study was to compare different invasive methods for Helicobacter pylori (H. pylori) detection, namely PCR for H. pylori specific ureC gene, Rapid urease test (RUT), and histopathological examination by modified Giemsa staining. METHODOLOGY: Endoscopic gastroduodenal biopsy materials were collected from dyspeptic patients who underwent endoscopic examination upon fulfilling the inclusion criteria. Three to four samples were collected from each patient after taking informed consent and proper clinical history. A rapid urease test (RUT) was done on spot with in-house RUT media from 1 specimen. One to two specimens were preserved in 10% formaldehyde for histopathology and PCR for ureC gene was done from 1 specimen. Collected biopsy specimens from gastric and duodenal mucosa of 142 patients were categorized as H. pylori-positive cases and H. pylori-negative cases based on the case definition used in the study upon positivity of 3 diagnostic tests. RESULTS: Among 142 biopsy specimens, 34.5% were categorized as H. pylori-positive cases, 35.2% as H. pylori-negative cases, and finally 30.2% as doubtful or indeterminate cases. Rapid urease test was the most sensitive method, closely followed by ureC gene PCR and histopathology, with a sensitivity of 94.2%, 83.0%, and 76.5%, respectively. Whereas histology was the most specific, having 98.0% specificity followed by 83.0% in PCR. RUT was the least specific, with 55.5% specificity. CONCLUSION: While histopathology could detect H. pylori infection with the highest specificity, for definitive diagnosis combination of any 2 methods should be used, if available. SAGE Publications 2022-10-28 /pmc/articles/PMC9619850/ /pubmed/36325107 http://dx.doi.org/10.1177/11786361221133947 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Brief Report Kismat, Saifa Tanni, Nusrat Nur Akhtar, Rokshana Roy, Chandan Kumar Rahman, Mohammad Mosiur Molla, Md. Maruf Ahmed Anwar, Shaheda Ahmed, Sharmeen Diagnosis and Comparison of Three Invasive Detection Methods for Helicobacter pylori Infection |
title | Diagnosis and Comparison of Three Invasive Detection Methods for
Helicobacter pylori Infection |
title_full | Diagnosis and Comparison of Three Invasive Detection Methods for
Helicobacter pylori Infection |
title_fullStr | Diagnosis and Comparison of Three Invasive Detection Methods for
Helicobacter pylori Infection |
title_full_unstemmed | Diagnosis and Comparison of Three Invasive Detection Methods for
Helicobacter pylori Infection |
title_short | Diagnosis and Comparison of Three Invasive Detection Methods for
Helicobacter pylori Infection |
title_sort | diagnosis and comparison of three invasive detection methods for
helicobacter pylori infection |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619850/ https://www.ncbi.nlm.nih.gov/pubmed/36325107 http://dx.doi.org/10.1177/11786361221133947 |
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