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Asymmetrical lineage introgression and recombination in populations of Aspergillus flavus: Implications for biological control

Aspergillus flavus is an agriculturally important fungus that causes ear rot of maize and produces aflatoxins, of which B(1) is the most carcinogenic naturally-produced compound. In the US, the management of aflatoxins includes the deployment of biological control agents that comprise two nonaflatox...

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Autores principales: Molo, Megan S., White, James B., Cornish, Vicki, Gell, Richard M., Baars, Oliver, Singh, Rakhi, Carbone, Mary Anna, Isakeit, Thomas, Wise, Kiersten A., Woloshuk, Charles P., Bluhm, Burton H., Horn, Bruce W., Heiniger, Ron W., Carbone, Ignazio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9620740/
https://www.ncbi.nlm.nih.gov/pubmed/36301851
http://dx.doi.org/10.1371/journal.pone.0276556
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author Molo, Megan S.
White, James B.
Cornish, Vicki
Gell, Richard M.
Baars, Oliver
Singh, Rakhi
Carbone, Mary Anna
Isakeit, Thomas
Wise, Kiersten A.
Woloshuk, Charles P.
Bluhm, Burton H.
Horn, Bruce W.
Heiniger, Ron W.
Carbone, Ignazio
author_facet Molo, Megan S.
White, James B.
Cornish, Vicki
Gell, Richard M.
Baars, Oliver
Singh, Rakhi
Carbone, Mary Anna
Isakeit, Thomas
Wise, Kiersten A.
Woloshuk, Charles P.
Bluhm, Burton H.
Horn, Bruce W.
Heiniger, Ron W.
Carbone, Ignazio
author_sort Molo, Megan S.
collection PubMed
description Aspergillus flavus is an agriculturally important fungus that causes ear rot of maize and produces aflatoxins, of which B(1) is the most carcinogenic naturally-produced compound. In the US, the management of aflatoxins includes the deployment of biological control agents that comprise two nonaflatoxigenic A. flavus strains, either Afla-Guard (member of lineage IB) or AF36 (lineage IC). We used genotyping-by-sequencing to examine the influence of both biocontrol agents on native populations of A. flavus in cornfields in Texas, North Carolina, Arkansas, and Indiana. This study examined up to 27,529 single-nucleotide polymorphisms (SNPs) in a total of 815 A. flavus isolates, and 353 genome-wide haplotypes sampled before biocontrol application, three months after biocontrol application, and up to three years after initial application. Here, we report that the two distinct A. flavus evolutionary lineages IB and IC differ significantly in their frequency distributions across states. We provide evidence of increased unidirectional gene flow from lineage IB into IC, inferred to be due to the applied Afla-Guard biocontrol strain. Genetic exchange and recombination of biocontrol strains with native strains was detected in as little as three months after biocontrol application and up to one and three years later. There was limited inter-lineage migration in the untreated fields. These findings suggest that biocontrol products that include strains from lineage IB offer the greatest potential for sustained reductions in aflatoxin levels over several years. This knowledge has important implications for developing new biocontrol strategies.
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spelling pubmed-96207402022-11-01 Asymmetrical lineage introgression and recombination in populations of Aspergillus flavus: Implications for biological control Molo, Megan S. White, James B. Cornish, Vicki Gell, Richard M. Baars, Oliver Singh, Rakhi Carbone, Mary Anna Isakeit, Thomas Wise, Kiersten A. Woloshuk, Charles P. Bluhm, Burton H. Horn, Bruce W. Heiniger, Ron W. Carbone, Ignazio PLoS One Research Article Aspergillus flavus is an agriculturally important fungus that causes ear rot of maize and produces aflatoxins, of which B(1) is the most carcinogenic naturally-produced compound. In the US, the management of aflatoxins includes the deployment of biological control agents that comprise two nonaflatoxigenic A. flavus strains, either Afla-Guard (member of lineage IB) or AF36 (lineage IC). We used genotyping-by-sequencing to examine the influence of both biocontrol agents on native populations of A. flavus in cornfields in Texas, North Carolina, Arkansas, and Indiana. This study examined up to 27,529 single-nucleotide polymorphisms (SNPs) in a total of 815 A. flavus isolates, and 353 genome-wide haplotypes sampled before biocontrol application, three months after biocontrol application, and up to three years after initial application. Here, we report that the two distinct A. flavus evolutionary lineages IB and IC differ significantly in their frequency distributions across states. We provide evidence of increased unidirectional gene flow from lineage IB into IC, inferred to be due to the applied Afla-Guard biocontrol strain. Genetic exchange and recombination of biocontrol strains with native strains was detected in as little as three months after biocontrol application and up to one and three years later. There was limited inter-lineage migration in the untreated fields. These findings suggest that biocontrol products that include strains from lineage IB offer the greatest potential for sustained reductions in aflatoxin levels over several years. This knowledge has important implications for developing new biocontrol strategies. Public Library of Science 2022-10-27 /pmc/articles/PMC9620740/ /pubmed/36301851 http://dx.doi.org/10.1371/journal.pone.0276556 Text en © 2022 Molo et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Molo, Megan S.
White, James B.
Cornish, Vicki
Gell, Richard M.
Baars, Oliver
Singh, Rakhi
Carbone, Mary Anna
Isakeit, Thomas
Wise, Kiersten A.
Woloshuk, Charles P.
Bluhm, Burton H.
Horn, Bruce W.
Heiniger, Ron W.
Carbone, Ignazio
Asymmetrical lineage introgression and recombination in populations of Aspergillus flavus: Implications for biological control
title Asymmetrical lineage introgression and recombination in populations of Aspergillus flavus: Implications for biological control
title_full Asymmetrical lineage introgression and recombination in populations of Aspergillus flavus: Implications for biological control
title_fullStr Asymmetrical lineage introgression and recombination in populations of Aspergillus flavus: Implications for biological control
title_full_unstemmed Asymmetrical lineage introgression and recombination in populations of Aspergillus flavus: Implications for biological control
title_short Asymmetrical lineage introgression and recombination in populations of Aspergillus flavus: Implications for biological control
title_sort asymmetrical lineage introgression and recombination in populations of aspergillus flavus: implications for biological control
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9620740/
https://www.ncbi.nlm.nih.gov/pubmed/36301851
http://dx.doi.org/10.1371/journal.pone.0276556
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