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Relationship between KIR genotypes and HLA-ligands with SARS-CoV-2 infection in the Saudi population

AIM: To ascertain whether killer cell immunoglobulin-like receptors (KIR) genes polymorphisms and HLA-I ligands are associated with COVID-19 in Saudi Arabia. METHODS: Eighty-seven COVID-19 patients who tested positive for SARS-CoV-2 and one hundred and fourteen healthy controls were enrolled in this...

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Autores principales: Alomar, Suliman, Alkhuriji, Afrah, Alkhulaifi, Fadwa M., Mansour, Lamjed, Al-jurayyan, Abdullah, Aldossari, Ghadeer S., Albalawi, Aishah Eid, Alanazi, Abdullah D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier B.V. on behalf of King Saud University. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9622466/
https://www.ncbi.nlm.nih.gov/pubmed/36338940
http://dx.doi.org/10.1016/j.jksus.2022.102416
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author Alomar, Suliman
Alkhuriji, Afrah
Alkhulaifi, Fadwa M.
Mansour, Lamjed
Al-jurayyan, Abdullah
Aldossari, Ghadeer S.
Albalawi, Aishah Eid
Alanazi, Abdullah D.
author_facet Alomar, Suliman
Alkhuriji, Afrah
Alkhulaifi, Fadwa M.
Mansour, Lamjed
Al-jurayyan, Abdullah
Aldossari, Ghadeer S.
Albalawi, Aishah Eid
Alanazi, Abdullah D.
author_sort Alomar, Suliman
collection PubMed
description AIM: To ascertain whether killer cell immunoglobulin-like receptors (KIR) genes polymorphisms and HLA-I ligands are associated with COVID-19 in Saudi Arabia. METHODS: Eighty-seven COVID-19 patients who tested positive for SARS-CoV-2 and one hundred and fourteen healthy controls were enrolled in this study for genotyping of the 16 KIR genes, HLA-C1 and -C2 allotypes and HLA-G 14-bp indels polymorphisms using the sequence specific primer polymerase chain reaction (SSP-PCR) method. KIR genotype frequency differences and combination KIR-HLA-C ligand were tested for significance. RESULTS: Framework genes KIR2DL4, KIR3DL2, KIR3DL3, and KIR3DP2 were present in all individuals. The frequencies of KIR2DL2 and KIR2D4 were higher in COVID-19 positive patients than in healthy individuals. The frequencies of the combination KIR2DL2-HLA-C2 was also significantly higher in patients affected by COVID-19 compared with healthy controls. CONCLUSION: It was found that the inhibitory KIR2DL2 gene in isolation or combined with its HLA-C2 ligand could be associated with susceptibility to COVID-19 in the Saudi population.
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spelling pubmed-96224662022-11-01 Relationship between KIR genotypes and HLA-ligands with SARS-CoV-2 infection in the Saudi population Alomar, Suliman Alkhuriji, Afrah Alkhulaifi, Fadwa M. Mansour, Lamjed Al-jurayyan, Abdullah Aldossari, Ghadeer S. Albalawi, Aishah Eid Alanazi, Abdullah D. J King Saud Univ Sci Article AIM: To ascertain whether killer cell immunoglobulin-like receptors (KIR) genes polymorphisms and HLA-I ligands are associated with COVID-19 in Saudi Arabia. METHODS: Eighty-seven COVID-19 patients who tested positive for SARS-CoV-2 and one hundred and fourteen healthy controls were enrolled in this study for genotyping of the 16 KIR genes, HLA-C1 and -C2 allotypes and HLA-G 14-bp indels polymorphisms using the sequence specific primer polymerase chain reaction (SSP-PCR) method. KIR genotype frequency differences and combination KIR-HLA-C ligand were tested for significance. RESULTS: Framework genes KIR2DL4, KIR3DL2, KIR3DL3, and KIR3DP2 were present in all individuals. The frequencies of KIR2DL2 and KIR2D4 were higher in COVID-19 positive patients than in healthy individuals. The frequencies of the combination KIR2DL2-HLA-C2 was also significantly higher in patients affected by COVID-19 compared with healthy controls. CONCLUSION: It was found that the inhibitory KIR2DL2 gene in isolation or combined with its HLA-C2 ligand could be associated with susceptibility to COVID-19 in the Saudi population. The Authors. Published by Elsevier B.V. on behalf of King Saud University. 2023-01 2022-11-01 /pmc/articles/PMC9622466/ /pubmed/36338940 http://dx.doi.org/10.1016/j.jksus.2022.102416 Text en © 2022 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Alomar, Suliman
Alkhuriji, Afrah
Alkhulaifi, Fadwa M.
Mansour, Lamjed
Al-jurayyan, Abdullah
Aldossari, Ghadeer S.
Albalawi, Aishah Eid
Alanazi, Abdullah D.
Relationship between KIR genotypes and HLA-ligands with SARS-CoV-2 infection in the Saudi population
title Relationship between KIR genotypes and HLA-ligands with SARS-CoV-2 infection in the Saudi population
title_full Relationship between KIR genotypes and HLA-ligands with SARS-CoV-2 infection in the Saudi population
title_fullStr Relationship between KIR genotypes and HLA-ligands with SARS-CoV-2 infection in the Saudi population
title_full_unstemmed Relationship between KIR genotypes and HLA-ligands with SARS-CoV-2 infection in the Saudi population
title_short Relationship between KIR genotypes and HLA-ligands with SARS-CoV-2 infection in the Saudi population
title_sort relationship between kir genotypes and hla-ligands with sars-cov-2 infection in the saudi population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9622466/
https://www.ncbi.nlm.nih.gov/pubmed/36338940
http://dx.doi.org/10.1016/j.jksus.2022.102416
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