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De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)–A therapeutically important orchid

Malaxis acuminata D. Don [=Crepidium acuminatum (D. Don) Szlach.] is an endangered medicinal orchid of the Ashtvarga group of plants in Ayurveda (Indian system of traditional medicine). Using a combination of aromatic cytokinin [meta-Topolin (mT)], plant biostimulant (chitosan), auxin [indole-3-buty...

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Autores principales: Bhattacharyya, Paromik, Sharma, Tanvi, Yadav, Abhinandan, Lalthafamkimi, Lucy, Ritu, Swarnkar, Mohit Kumar, Joshi, Robin, Shankar, Ravi, Kumar, Sanjay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623264/
https://www.ncbi.nlm.nih.gov/pubmed/36330257
http://dx.doi.org/10.3389/fpls.2022.954467
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author Bhattacharyya, Paromik
Sharma, Tanvi
Yadav, Abhinandan
Lalthafamkimi, Lucy
Ritu,
Swarnkar, Mohit Kumar
Joshi, Robin
Shankar, Ravi
Kumar, Sanjay
author_facet Bhattacharyya, Paromik
Sharma, Tanvi
Yadav, Abhinandan
Lalthafamkimi, Lucy
Ritu,
Swarnkar, Mohit Kumar
Joshi, Robin
Shankar, Ravi
Kumar, Sanjay
author_sort Bhattacharyya, Paromik
collection PubMed
description Malaxis acuminata D. Don [=Crepidium acuminatum (D. Don) Szlach.] is an endangered medicinal orchid of the Ashtvarga group of plants in Ayurveda (Indian system of traditional medicine). Using a combination of aromatic cytokinin [meta-Topolin (mT)], plant biostimulant (chitosan), auxin [indole-3-butyric acid (IBA)], and a phenolic elicitor [phloroglucinol (PG)], plants of M. acuminata were regenerated in vitro for mass multiplication. The present research reveals the first-ever transcriptome of M. acuminata. A total of 43,111 transcripts encoding 23,951 unigenes were assembled de novo from a total of 815.02 million reads obtained from leaf and pseudobulb of in vitro raised M. acuminata. Expression analysis of genes associated with β-sitosterol and eugenol biosynthesis in leaf and pseudobulb provided vital clues for differential accumulation of metabolites in M. acuminata. Ultra-performance liquid chromatography (UPLC) confirmed higher amounts of β-sitosterol and eugenol content in the leaf as compared to the pseudobulb. Differential expression of transcripts related to starch and sucrose metabolism, plant hormone signal transduction, diterpenoid biosynthesis, phenylalanine metabolism, stilbenoid, diarylheptanoid, and gingerol biosynthesis suggested the operation of differential metabolic pathways in leaf and pseudobulb. The present research provides valuable information on the biosynthesis of secondary metabolites in M. acuminata, which could be used for advanced metabolite bioprospection using cell suspension culture and bioreactor-based approaches. Data also suggested that leaf tissues rather than pseudobulb can be used as an alternate source of bioactive metabolites thereby shifting the need for harvesting the pseudobulb. This will further facilitate the conservation and sustainable utilization of this highly valued medicinal orchid.
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spelling pubmed-96232642022-11-02 De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)–A therapeutically important orchid Bhattacharyya, Paromik Sharma, Tanvi Yadav, Abhinandan Lalthafamkimi, Lucy Ritu, Swarnkar, Mohit Kumar Joshi, Robin Shankar, Ravi Kumar, Sanjay Front Plant Sci Plant Science Malaxis acuminata D. Don [=Crepidium acuminatum (D. Don) Szlach.] is an endangered medicinal orchid of the Ashtvarga group of plants in Ayurveda (Indian system of traditional medicine). Using a combination of aromatic cytokinin [meta-Topolin (mT)], plant biostimulant (chitosan), auxin [indole-3-butyric acid (IBA)], and a phenolic elicitor [phloroglucinol (PG)], plants of M. acuminata were regenerated in vitro for mass multiplication. The present research reveals the first-ever transcriptome of M. acuminata. A total of 43,111 transcripts encoding 23,951 unigenes were assembled de novo from a total of 815.02 million reads obtained from leaf and pseudobulb of in vitro raised M. acuminata. Expression analysis of genes associated with β-sitosterol and eugenol biosynthesis in leaf and pseudobulb provided vital clues for differential accumulation of metabolites in M. acuminata. Ultra-performance liquid chromatography (UPLC) confirmed higher amounts of β-sitosterol and eugenol content in the leaf as compared to the pseudobulb. Differential expression of transcripts related to starch and sucrose metabolism, plant hormone signal transduction, diterpenoid biosynthesis, phenylalanine metabolism, stilbenoid, diarylheptanoid, and gingerol biosynthesis suggested the operation of differential metabolic pathways in leaf and pseudobulb. The present research provides valuable information on the biosynthesis of secondary metabolites in M. acuminata, which could be used for advanced metabolite bioprospection using cell suspension culture and bioreactor-based approaches. Data also suggested that leaf tissues rather than pseudobulb can be used as an alternate source of bioactive metabolites thereby shifting the need for harvesting the pseudobulb. This will further facilitate the conservation and sustainable utilization of this highly valued medicinal orchid. Frontiers Media S.A. 2022-10-18 /pmc/articles/PMC9623264/ /pubmed/36330257 http://dx.doi.org/10.3389/fpls.2022.954467 Text en Copyright © 2022 Bhattacharyya, Sharma, Yadav, Lalthafamkimi, Ritu, Swarnkar, Joshi, Shankar and Kumar. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bhattacharyya, Paromik
Sharma, Tanvi
Yadav, Abhinandan
Lalthafamkimi, Lucy
Ritu,
Swarnkar, Mohit Kumar
Joshi, Robin
Shankar, Ravi
Kumar, Sanjay
De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)–A therapeutically important orchid
title De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)–A therapeutically important orchid
title_full De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)–A therapeutically important orchid
title_fullStr De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)–A therapeutically important orchid
title_full_unstemmed De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)–A therapeutically important orchid
title_short De novo transcriptome based insights into secondary metabolite biosynthesis in Malaxis acuminata (Jeevak)–A therapeutically important orchid
title_sort de novo transcriptome based insights into secondary metabolite biosynthesis in malaxis acuminata (jeevak)–a therapeutically important orchid
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623264/
https://www.ncbi.nlm.nih.gov/pubmed/36330257
http://dx.doi.org/10.3389/fpls.2022.954467
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