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Pitfalls in complement analysis: A systematic literature review of assessing complement activation
BACKGROUND: The complement system is an essential component of our innate defense and plays a vital role in the pathogenesis of many diseases. Assessment of complement activation is critical in monitoring both disease progression and response to therapy. Complement analysis requires accurate and sta...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623276/ https://www.ncbi.nlm.nih.gov/pubmed/36330514 http://dx.doi.org/10.3389/fimmu.2022.1007102 |
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author | Brandwijk, Ricardo J. M. G. E. Michels, Marloes A. H. M. van Rossum, Mara de Nooijer, Aline H. Nilsson, Per H. de Bruin, Wieke C. C. Toonen, Erik J. M. |
author_facet | Brandwijk, Ricardo J. M. G. E. Michels, Marloes A. H. M. van Rossum, Mara de Nooijer, Aline H. Nilsson, Per H. de Bruin, Wieke C. C. Toonen, Erik J. M. |
author_sort | Brandwijk, Ricardo J. M. G. E. |
collection | PubMed |
description | BACKGROUND: The complement system is an essential component of our innate defense and plays a vital role in the pathogenesis of many diseases. Assessment of complement activation is critical in monitoring both disease progression and response to therapy. Complement analysis requires accurate and standardized sampling and assay procedures, which has proven to be challenging. OBJECTIVE: We performed a systematic analysis of the current methods used to assess complement components and reviewed whether the identified studies performed their complement measurements according to the recommended practice regarding pre-analytical sample handling and assay technique. Results are supplemented with own data regarding the assessment of key complement biomarkers to illustrate the importance of accurate sampling and measuring of complement components. METHODS: A literature search using the Pubmed/MEDLINE database was performed focusing on studies measuring the key complement components C3, C5 and/or their split products and/or the soluble variant of the terminal C5b-9 complement complex (sTCC) in human blood samples that were published between February 2017 and February 2022. The identified studies were reviewed whether they had used the correct sample type and techniques for their analyses. RESULTS: A total of 92 out of 376 studies were selected for full-text analysis. Forty-five studies (49%) were identified as using the correct sample type and techniques for their complement analyses, while 25 studies (27%) did not use the correct sample type or technique. For 22 studies (24%), it was not specified which sample type was used. CONCLUSION: A substantial part of the reviewed studies did not use the appropriate sample type for assessing complement activation or did not mention which sample type was used. This deviation from the standardized procedure can lead to misinterpretation of complement biomarker levels and hampers proper comparison of complement measurements between studies. Therefore, this study underlines the necessity of general guidelines for accurate and standardized complement analysis |
format | Online Article Text |
id | pubmed-9623276 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96232762022-11-02 Pitfalls in complement analysis: A systematic literature review of assessing complement activation Brandwijk, Ricardo J. M. G. E. Michels, Marloes A. H. M. van Rossum, Mara de Nooijer, Aline H. Nilsson, Per H. de Bruin, Wieke C. C. Toonen, Erik J. M. Front Immunol Immunology BACKGROUND: The complement system is an essential component of our innate defense and plays a vital role in the pathogenesis of many diseases. Assessment of complement activation is critical in monitoring both disease progression and response to therapy. Complement analysis requires accurate and standardized sampling and assay procedures, which has proven to be challenging. OBJECTIVE: We performed a systematic analysis of the current methods used to assess complement components and reviewed whether the identified studies performed their complement measurements according to the recommended practice regarding pre-analytical sample handling and assay technique. Results are supplemented with own data regarding the assessment of key complement biomarkers to illustrate the importance of accurate sampling and measuring of complement components. METHODS: A literature search using the Pubmed/MEDLINE database was performed focusing on studies measuring the key complement components C3, C5 and/or their split products and/or the soluble variant of the terminal C5b-9 complement complex (sTCC) in human blood samples that were published between February 2017 and February 2022. The identified studies were reviewed whether they had used the correct sample type and techniques for their analyses. RESULTS: A total of 92 out of 376 studies were selected for full-text analysis. Forty-five studies (49%) were identified as using the correct sample type and techniques for their complement analyses, while 25 studies (27%) did not use the correct sample type or technique. For 22 studies (24%), it was not specified which sample type was used. CONCLUSION: A substantial part of the reviewed studies did not use the appropriate sample type for assessing complement activation or did not mention which sample type was used. This deviation from the standardized procedure can lead to misinterpretation of complement biomarker levels and hampers proper comparison of complement measurements between studies. Therefore, this study underlines the necessity of general guidelines for accurate and standardized complement analysis Frontiers Media S.A. 2022-10-18 /pmc/articles/PMC9623276/ /pubmed/36330514 http://dx.doi.org/10.3389/fimmu.2022.1007102 Text en Copyright © 2022 Brandwijk, Michels, van Rossum, de Nooijer, Nilsson, de Bruin and Toonen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Brandwijk, Ricardo J. M. G. E. Michels, Marloes A. H. M. van Rossum, Mara de Nooijer, Aline H. Nilsson, Per H. de Bruin, Wieke C. C. Toonen, Erik J. M. Pitfalls in complement analysis: A systematic literature review of assessing complement activation |
title | Pitfalls in complement analysis: A systematic literature review of assessing complement activation |
title_full | Pitfalls in complement analysis: A systematic literature review of assessing complement activation |
title_fullStr | Pitfalls in complement analysis: A systematic literature review of assessing complement activation |
title_full_unstemmed | Pitfalls in complement analysis: A systematic literature review of assessing complement activation |
title_short | Pitfalls in complement analysis: A systematic literature review of assessing complement activation |
title_sort | pitfalls in complement analysis: a systematic literature review of assessing complement activation |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623276/ https://www.ncbi.nlm.nih.gov/pubmed/36330514 http://dx.doi.org/10.3389/fimmu.2022.1007102 |
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