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ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation

Cell migration plays an important role in multicellular organism development. The cellular slime mold Dictyostelium discoideum is a useful model organism for the study of cell migration during development. Although cellular ATP levels are known to determine cell fate during development, the underlyi...

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Autores principales: Hiraoka, Haruka, Wang, Jiewen, Nakano, Tadashi, Hirano, Yasuhiro, Yamazaki, Shinichi, Hiraoka, Yasushi, Haraguchi, Tokuko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623536/
https://www.ncbi.nlm.nih.gov/pubmed/36054629
http://dx.doi.org/10.1002/2211-5463.13480
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author Hiraoka, Haruka
Wang, Jiewen
Nakano, Tadashi
Hirano, Yasuhiro
Yamazaki, Shinichi
Hiraoka, Yasushi
Haraguchi, Tokuko
author_facet Hiraoka, Haruka
Wang, Jiewen
Nakano, Tadashi
Hirano, Yasuhiro
Yamazaki, Shinichi
Hiraoka, Yasushi
Haraguchi, Tokuko
author_sort Hiraoka, Haruka
collection PubMed
description Cell migration plays an important role in multicellular organism development. The cellular slime mold Dictyostelium discoideum is a useful model organism for the study of cell migration during development. Although cellular ATP levels are known to determine cell fate during development, the underlying mechanism remains unclear. Here, we report that ATP‐rich cells efficiently move to the central tip region of the mound against rotational movement during the mound phase. A simulation analysis based on an agent‐based model reproduces the movement of ATP‐rich cells observed in the experiments. These findings indicate that ATP‐rich cells have the ability to move against the bulk flow of cells, suggesting a mechanism by which high ATP levels determine the cell fate of differentiation.
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spelling pubmed-96235362022-11-02 ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation Hiraoka, Haruka Wang, Jiewen Nakano, Tadashi Hirano, Yasuhiro Yamazaki, Shinichi Hiraoka, Yasushi Haraguchi, Tokuko FEBS Open Bio Research Articles Cell migration plays an important role in multicellular organism development. The cellular slime mold Dictyostelium discoideum is a useful model organism for the study of cell migration during development. Although cellular ATP levels are known to determine cell fate during development, the underlying mechanism remains unclear. Here, we report that ATP‐rich cells efficiently move to the central tip region of the mound against rotational movement during the mound phase. A simulation analysis based on an agent‐based model reproduces the movement of ATP‐rich cells observed in the experiments. These findings indicate that ATP‐rich cells have the ability to move against the bulk flow of cells, suggesting a mechanism by which high ATP levels determine the cell fate of differentiation. John Wiley and Sons Inc. 2022-09-23 /pmc/articles/PMC9623536/ /pubmed/36054629 http://dx.doi.org/10.1002/2211-5463.13480 Text en © 2022 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Hiraoka, Haruka
Wang, Jiewen
Nakano, Tadashi
Hirano, Yasuhiro
Yamazaki, Shinichi
Hiraoka, Yasushi
Haraguchi, Tokuko
ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation
title ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation
title_full ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation
title_fullStr ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation
title_full_unstemmed ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation
title_short ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation
title_sort atp levels influence cell movement during the mound phase in dictyostelium discoideum as revealed by atp visualization and simulation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623536/
https://www.ncbi.nlm.nih.gov/pubmed/36054629
http://dx.doi.org/10.1002/2211-5463.13480
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