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Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves
This study examined the effects of changes in rumen fermentation during the weaning transition on abundance of transporters involved in volatile fatty acid (VFA) absorption or intracellular pH homeostasis. Holstein bull calves (n = 27) were assigned to 1 of 3 treatment groups in a randomized, comple...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623740/ https://www.ncbi.nlm.nih.gov/pubmed/36337096 http://dx.doi.org/10.3168/jdsc.2021-0088 |
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author | Hiltz, Rebecca L. McCurdy, Dana E. Moreland, Steven Klanderman, Keith Laarman, Anne H. |
author_facet | Hiltz, Rebecca L. McCurdy, Dana E. Moreland, Steven Klanderman, Keith Laarman, Anne H. |
author_sort | Hiltz, Rebecca L. |
collection | PubMed |
description | This study examined the effects of changes in rumen fermentation during the weaning transition on abundance of transporters involved in volatile fatty acid (VFA) absorption or intracellular pH homeostasis. Holstein bull calves (n = 27) were assigned to 1 of 3 treatment groups in a randomized, complete block design: 2 preweaning groups [animals fed milk only (PRE-M) or milk, calf starter, and hay (PRE-S)] and 1 postweaning group (animals fed milk, starter, and hay with a 2-wk weaning transition; POST-S). Calves were euthanized at 42 d of age (PRE-M and PRE-S) or at 63 d of age (POST-S), and rumen epithelium and rumen fluid samples were collected. Rumen fluid was analyzed for VFA concentration, and rumen epithelium was analyzed for the abundance of VFA transporter monocarboxylate transporter isoform 1 (MCT1) and the intracellular pH regulators sodium bicarbonate co-transporter 1 (NBC1) and sodium-proton exchanger 3 (NHE3) protein. Preweaning, total VFA concentrations tended to increase and NBC1 abundance increased with starter intake. Between pre- and postweaning, total VFA concentrations increased but NHE3 protein abundance decreased. In calves, rumen epithelial development during the weaning transition appears to show more pronounced changes in intracellular pH homeostasis than in VFA transport capacity. |
format | Online Article Text |
id | pubmed-9623740 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96237402022-11-04 Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves Hiltz, Rebecca L. McCurdy, Dana E. Moreland, Steven Klanderman, Keith Laarman, Anne H. JDS Commun Animal Nutrition and Farm Systems This study examined the effects of changes in rumen fermentation during the weaning transition on abundance of transporters involved in volatile fatty acid (VFA) absorption or intracellular pH homeostasis. Holstein bull calves (n = 27) were assigned to 1 of 3 treatment groups in a randomized, complete block design: 2 preweaning groups [animals fed milk only (PRE-M) or milk, calf starter, and hay (PRE-S)] and 1 postweaning group (animals fed milk, starter, and hay with a 2-wk weaning transition; POST-S). Calves were euthanized at 42 d of age (PRE-M and PRE-S) or at 63 d of age (POST-S), and rumen epithelium and rumen fluid samples were collected. Rumen fluid was analyzed for VFA concentration, and rumen epithelium was analyzed for the abundance of VFA transporter monocarboxylate transporter isoform 1 (MCT1) and the intracellular pH regulators sodium bicarbonate co-transporter 1 (NBC1) and sodium-proton exchanger 3 (NHE3) protein. Preweaning, total VFA concentrations tended to increase and NBC1 abundance increased with starter intake. Between pre- and postweaning, total VFA concentrations increased but NHE3 protein abundance decreased. In calves, rumen epithelial development during the weaning transition appears to show more pronounced changes in intracellular pH homeostasis than in VFA transport capacity. Elsevier 2021-09-13 /pmc/articles/PMC9623740/ /pubmed/36337096 http://dx.doi.org/10.3168/jdsc.2021-0088 Text en © 2021. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Animal Nutrition and Farm Systems Hiltz, Rebecca L. McCurdy, Dana E. Moreland, Steven Klanderman, Keith Laarman, Anne H. Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves |
title | Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves |
title_full | Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves |
title_fullStr | Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves |
title_full_unstemmed | Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves |
title_short | Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves |
title_sort | effects of weaning on regulators of volatile fatty acid absorption and intracellular ph in holstein calves |
topic | Animal Nutrition and Farm Systems |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623740/ https://www.ncbi.nlm.nih.gov/pubmed/36337096 http://dx.doi.org/10.3168/jdsc.2021-0088 |
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