Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea

Host genotype is important for enterotoxigenic E. coli (ETEC) susceptibility. We conducted two trials to evaluate the effect of CHCF1 genotype on incidence of ETEC diarrhea. In trial 1 (n = 15 pigs), pigs were inoculated with 10(8) CFU or 10(10) CFU doses of an ETEC F4ac strain. In trial 2 (n = 33 p...

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Autores principales: Rydal, Martin Peter, Gambino, Michela, Jørgensen, Claus Bøttcher, Poulsen, Louise Ladefoged, Brøndsted, Lone, Nielsen, Jens Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624054/
https://www.ncbi.nlm.nih.gov/pubmed/36320033
http://dx.doi.org/10.1186/s12917-022-03474-3
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author Rydal, Martin Peter
Gambino, Michela
Jørgensen, Claus Bøttcher
Poulsen, Louise Ladefoged
Brøndsted, Lone
Nielsen, Jens Peter
author_facet Rydal, Martin Peter
Gambino, Michela
Jørgensen, Claus Bøttcher
Poulsen, Louise Ladefoged
Brøndsted, Lone
Nielsen, Jens Peter
author_sort Rydal, Martin Peter
collection PubMed
description Host genotype is important for enterotoxigenic E. coli (ETEC) susceptibility. We conducted two trials to evaluate the effect of CHCF1 genotype on incidence of ETEC diarrhea. In trial 1 (n = 15 pigs), pigs were inoculated with 10(8) CFU or 10(10) CFU doses of an ETEC F4ac strain. In trial 2 (n = 33 pigs), pigs were inoculated with ETEC F4ab or F4ac. Across trials, all inoculated pigs that developed ETEC diarrhea were CHCF1 heterozygous susceptible (6/6). No inoculated CHCF1 homozygous resistant pigs developed ETEC diarrhea (0/26). Susceptibility towards ETEC F4ac/ab infection might correspond with CHCF1 genotype. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03474-3.
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spelling pubmed-96240542022-11-02 Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea Rydal, Martin Peter Gambino, Michela Jørgensen, Claus Bøttcher Poulsen, Louise Ladefoged Brøndsted, Lone Nielsen, Jens Peter BMC Vet Res Research Host genotype is important for enterotoxigenic E. coli (ETEC) susceptibility. We conducted two trials to evaluate the effect of CHCF1 genotype on incidence of ETEC diarrhea. In trial 1 (n = 15 pigs), pigs were inoculated with 10(8) CFU or 10(10) CFU doses of an ETEC F4ac strain. In trial 2 (n = 33 pigs), pigs were inoculated with ETEC F4ab or F4ac. Across trials, all inoculated pigs that developed ETEC diarrhea were CHCF1 heterozygous susceptible (6/6). No inoculated CHCF1 homozygous resistant pigs developed ETEC diarrhea (0/26). Susceptibility towards ETEC F4ac/ab infection might correspond with CHCF1 genotype. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12917-022-03474-3. BioMed Central 2022-11-01 /pmc/articles/PMC9624054/ /pubmed/36320033 http://dx.doi.org/10.1186/s12917-022-03474-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Rydal, Martin Peter
Gambino, Michela
Jørgensen, Claus Bøttcher
Poulsen, Louise Ladefoged
Brøndsted, Lone
Nielsen, Jens Peter
Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea
title Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea
title_full Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea
title_fullStr Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea
title_full_unstemmed Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea
title_short Pilot study on CHCF1 genotype in a pig challenge model for enterotoxigenic Escherichia coli F4ab/ac associated post-weaning diarrhea
title_sort pilot study on chcf1 genotype in a pig challenge model for enterotoxigenic escherichia coli f4ab/ac associated post-weaning diarrhea
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624054/
https://www.ncbi.nlm.nih.gov/pubmed/36320033
http://dx.doi.org/10.1186/s12917-022-03474-3
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