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Analysis of different adipose depot gene expression in cachectic patients with gastric cancer

PURPOSE: This study aimed to identify the differentially expressed genes (DEGs) that contributed to the different amount of fat loss between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) among cachectic patients. METHODS: RNA sequencing was performed and bioinformatic tools wer...

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Autores principales: Han, Jun, Ding, Zuoyou, Zhuang, Qiulin, Shen, Lei, Yang, Fan, Sah, Szechun, Wu, Guohao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624057/
https://www.ncbi.nlm.nih.gov/pubmed/36316707
http://dx.doi.org/10.1186/s12986-022-00708-x
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author Han, Jun
Ding, Zuoyou
Zhuang, Qiulin
Shen, Lei
Yang, Fan
Sah, Szechun
Wu, Guohao
author_facet Han, Jun
Ding, Zuoyou
Zhuang, Qiulin
Shen, Lei
Yang, Fan
Sah, Szechun
Wu, Guohao
author_sort Han, Jun
collection PubMed
description PURPOSE: This study aimed to identify the differentially expressed genes (DEGs) that contributed to the different amount of fat loss between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) among cachectic patients. METHODS: RNA sequencing was performed and bioinformatic tools were utilized to analyze the biological functions and construct regulation networks of DEGs. We presumed that iroquois homeobox 1 (IRX1) to be a hub gene and analyzed its clinical significance. Mouse model of cancer cachexia was established and differences between SAT and VAT were compared. The function of IRX1 on lipid metabolism was clarified by Oil Red O staining, qRT-PCR, and Western blotting in adipocytes. RESULTS: A total of 455 DEGs were screened between SAT and VAT in cachectic patients. Several hub genes were selected and IRX1 was presumed to contribute to the pathological difference between SAT and VAT in cancer cachexia. Patients with higher expression of IRX1 in SAT than VAT revealed significantly higher weight loss, IL-6 and TNF-α, as well as lower BMI, SAT, and VAT area. IRX1 expression in SAT was negatively correlated with SAT area. In cachectic mice, the expression of IRX1 in SAT was significantly higher than that in VAT. The inhibition effect on adipogenesis exerted by IRX1 was also proved in vitro. CONCLUSION: These data supported that DEGs contribute to the different degrees of fat loss among adipose depots in cachectic patients. IRX1 in SAT promoted fat loss by inhibiting adipocyte differentiation and adipogenesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-022-00708-x.
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spelling pubmed-96240572022-11-02 Analysis of different adipose depot gene expression in cachectic patients with gastric cancer Han, Jun Ding, Zuoyou Zhuang, Qiulin Shen, Lei Yang, Fan Sah, Szechun Wu, Guohao Nutr Metab (Lond) Research PURPOSE: This study aimed to identify the differentially expressed genes (DEGs) that contributed to the different amount of fat loss between subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) among cachectic patients. METHODS: RNA sequencing was performed and bioinformatic tools were utilized to analyze the biological functions and construct regulation networks of DEGs. We presumed that iroquois homeobox 1 (IRX1) to be a hub gene and analyzed its clinical significance. Mouse model of cancer cachexia was established and differences between SAT and VAT were compared. The function of IRX1 on lipid metabolism was clarified by Oil Red O staining, qRT-PCR, and Western blotting in adipocytes. RESULTS: A total of 455 DEGs were screened between SAT and VAT in cachectic patients. Several hub genes were selected and IRX1 was presumed to contribute to the pathological difference between SAT and VAT in cancer cachexia. Patients with higher expression of IRX1 in SAT than VAT revealed significantly higher weight loss, IL-6 and TNF-α, as well as lower BMI, SAT, and VAT area. IRX1 expression in SAT was negatively correlated with SAT area. In cachectic mice, the expression of IRX1 in SAT was significantly higher than that in VAT. The inhibition effect on adipogenesis exerted by IRX1 was also proved in vitro. CONCLUSION: These data supported that DEGs contribute to the different degrees of fat loss among adipose depots in cachectic patients. IRX1 in SAT promoted fat loss by inhibiting adipocyte differentiation and adipogenesis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12986-022-00708-x. BioMed Central 2022-10-31 /pmc/articles/PMC9624057/ /pubmed/36316707 http://dx.doi.org/10.1186/s12986-022-00708-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Han, Jun
Ding, Zuoyou
Zhuang, Qiulin
Shen, Lei
Yang, Fan
Sah, Szechun
Wu, Guohao
Analysis of different adipose depot gene expression in cachectic patients with gastric cancer
title Analysis of different adipose depot gene expression in cachectic patients with gastric cancer
title_full Analysis of different adipose depot gene expression in cachectic patients with gastric cancer
title_fullStr Analysis of different adipose depot gene expression in cachectic patients with gastric cancer
title_full_unstemmed Analysis of different adipose depot gene expression in cachectic patients with gastric cancer
title_short Analysis of different adipose depot gene expression in cachectic patients with gastric cancer
title_sort analysis of different adipose depot gene expression in cachectic patients with gastric cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624057/
https://www.ncbi.nlm.nih.gov/pubmed/36316707
http://dx.doi.org/10.1186/s12986-022-00708-x
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