Cargando…

Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei

One hundred proteins in Saccharomyces cerevisiae are known to contain iron. These proteins are found mainly in mitochondria, cytosol, nuclei, endoplasmic reticula, and vacuoles. Cells also contain non-proteinaceous low-molecular-mass labile iron pools (LFePs). How each molecular iron species interac...

Descripción completa

Detalles Bibliográficos
Autores principales: Lindahl, Paul A, Vali, Shaik Waseem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624242/
https://www.ncbi.nlm.nih.gov/pubmed/36214417
http://dx.doi.org/10.1093/mtomcs/mfac080
_version_ 1784822188541476864
author Lindahl, Paul A
Vali, Shaik Waseem
author_facet Lindahl, Paul A
Vali, Shaik Waseem
author_sort Lindahl, Paul A
collection PubMed
description One hundred proteins in Saccharomyces cerevisiae are known to contain iron. These proteins are found mainly in mitochondria, cytosol, nuclei, endoplasmic reticula, and vacuoles. Cells also contain non-proteinaceous low-molecular-mass labile iron pools (LFePs). How each molecular iron species interacts on the cellular or systems’ level is underdeveloped as doing so would require considering the entire iron content of the cell—the ironome. In this paper, Mössbauer (MB) spectroscopy was used to probe the ironome of yeast. MB spectra of whole cells and isolated organelles were predicted by summing the spectral contribution of each iron-containing species in the cell. Simulations required input from published proteomics and microscopy data, as well as from previous spectroscopic and redox characterization of individual iron-containing proteins. Composite simulations were compared to experimentally determined spectra. Simulated MB spectra of non-proteinaceous iron pools in the cell were assumed to account for major differences between simulated and experimental spectra of whole cells and isolated mitochondria and vacuoles. Nuclei were predicted to contain ∼30 μM iron, mostly in the form of [Fe(4)S(4)] clusters. This was experimentally confirmed by isolating nuclei from (57)Fe-enriched cells and obtaining the first MB spectra of the organelle. This study provides the first semi-quantitative estimate of all concentrations of iron-containing proteins and non-proteinaceous species in yeast, as well as a novel approach to spectroscopically characterizing LFePs.
format Online
Article
Text
id pubmed-9624242
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-96242422022-11-02 Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei Lindahl, Paul A Vali, Shaik Waseem Metallomics Paper One hundred proteins in Saccharomyces cerevisiae are known to contain iron. These proteins are found mainly in mitochondria, cytosol, nuclei, endoplasmic reticula, and vacuoles. Cells also contain non-proteinaceous low-molecular-mass labile iron pools (LFePs). How each molecular iron species interacts on the cellular or systems’ level is underdeveloped as doing so would require considering the entire iron content of the cell—the ironome. In this paper, Mössbauer (MB) spectroscopy was used to probe the ironome of yeast. MB spectra of whole cells and isolated organelles were predicted by summing the spectral contribution of each iron-containing species in the cell. Simulations required input from published proteomics and microscopy data, as well as from previous spectroscopic and redox characterization of individual iron-containing proteins. Composite simulations were compared to experimentally determined spectra. Simulated MB spectra of non-proteinaceous iron pools in the cell were assumed to account for major differences between simulated and experimental spectra of whole cells and isolated mitochondria and vacuoles. Nuclei were predicted to contain ∼30 μM iron, mostly in the form of [Fe(4)S(4)] clusters. This was experimentally confirmed by isolating nuclei from (57)Fe-enriched cells and obtaining the first MB spectra of the organelle. This study provides the first semi-quantitative estimate of all concentrations of iron-containing proteins and non-proteinaceous species in yeast, as well as a novel approach to spectroscopically characterizing LFePs. Oxford University Press 2022-10-10 /pmc/articles/PMC9624242/ /pubmed/36214417 http://dx.doi.org/10.1093/mtomcs/mfac080 Text en © The Author(s) 2022. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Paper
Lindahl, Paul A
Vali, Shaik Waseem
Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei
title Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei
title_full Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei
title_fullStr Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei
title_full_unstemmed Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei
title_short Mössbauer-based molecular-level decomposition of the Saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei
title_sort mössbauer-based molecular-level decomposition of the saccharomyces cerevisiae ironome, and preliminary characterization of isolated nuclei
topic Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624242/
https://www.ncbi.nlm.nih.gov/pubmed/36214417
http://dx.doi.org/10.1093/mtomcs/mfac080
work_keys_str_mv AT lindahlpaula mossbauerbasedmolecularleveldecompositionofthesaccharomycescerevisiaeironomeandpreliminarycharacterizationofisolatednuclei
AT valishaikwaseem mossbauerbasedmolecularleveldecompositionofthesaccharomycescerevisiaeironomeandpreliminarycharacterizationofisolatednuclei