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Benzyldimethyldodecyl Ammonium Chloride Doped Dental Adhesive: Impact on Core’s Properties, Biosafety, and Antibacterial/Bonding Performance after Aging

Current dental adhesives lack antibacterial properties. This study aimed to explore the effect of incorporating benzyldimethyldodecyl ammonium chloride (BDMDAC) on the degree of conversion, contact angle, ultimate tensile strength (UTS), microtensile bond strength (µTBS), cytotoxicity, antibacterial...

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Autores principales: Mokeem, Lamia Sami, Balhaddad, Abdulrahman A., Garcia, Isadora Martini, Collares, Fabrício Mezzomo, Melo, Mary Anne S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624347/
https://www.ncbi.nlm.nih.gov/pubmed/36278659
http://dx.doi.org/10.3390/jfb13040190
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author Mokeem, Lamia Sami
Balhaddad, Abdulrahman A.
Garcia, Isadora Martini
Collares, Fabrício Mezzomo
Melo, Mary Anne S.
author_facet Mokeem, Lamia Sami
Balhaddad, Abdulrahman A.
Garcia, Isadora Martini
Collares, Fabrício Mezzomo
Melo, Mary Anne S.
author_sort Mokeem, Lamia Sami
collection PubMed
description Current dental adhesives lack antibacterial properties. This study aimed to explore the effect of incorporating benzyldimethyldodecyl ammonium chloride (BDMDAC) on the degree of conversion, contact angle, ultimate tensile strength (UTS), microtensile bond strength (µTBS), cytotoxicity, antibacterial and bonding performance after artificial aging. A dental adhesive was doped with BDMDAC in the concentration range of 1–5 wt.%. For antibacterial assays, the BDMDAC compound was subject to planktonic cells of Streptococcus mutans. Then, after incorporation into the dental adhesive, an S. mutans biofilm model was used to grow 48 h-mature biofilms. The biofilms grown over the formulated materials were assessed by colony-forming unit (CFU) counting assay and fluorescence microscopy staining. In addition, the cytotoxicity was evaluated. Samples were subjected to 10,000 thermal cycles for aging and evaluated by UTS, µTBS, and CFU. Incorporating BDMDAC did not increase the cytotoxicity or change the physical properties when the mass fraction of the BDMDAC was 1–5 wt.%. The UTS of BDMDAC-doped adhesives was not impaired immediately or over time. A significant bacterial reduction was obtained for the mass fraction of the BDMDAC greater than 3 wt.%. However, the BDMDAC-doped adhesives did not offer an antibacterial effect after artificial aging. The overall results indicate that the BDMDAC strategy has the potential to control of microbial growth of cariogenic planktonic cells and biofilms. However, other new technological approaches are needed to overcome the deleterious effect of BDMDAC release over time such as those based on the principle of drug delivery systems whereby the BDMDAC is transported on microparticles or core shells, providing tangible benefits to oral health over time.
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spelling pubmed-96243472022-11-02 Benzyldimethyldodecyl Ammonium Chloride Doped Dental Adhesive: Impact on Core’s Properties, Biosafety, and Antibacterial/Bonding Performance after Aging Mokeem, Lamia Sami Balhaddad, Abdulrahman A. Garcia, Isadora Martini Collares, Fabrício Mezzomo Melo, Mary Anne S. J Funct Biomater Article Current dental adhesives lack antibacterial properties. This study aimed to explore the effect of incorporating benzyldimethyldodecyl ammonium chloride (BDMDAC) on the degree of conversion, contact angle, ultimate tensile strength (UTS), microtensile bond strength (µTBS), cytotoxicity, antibacterial and bonding performance after artificial aging. A dental adhesive was doped with BDMDAC in the concentration range of 1–5 wt.%. For antibacterial assays, the BDMDAC compound was subject to planktonic cells of Streptococcus mutans. Then, after incorporation into the dental adhesive, an S. mutans biofilm model was used to grow 48 h-mature biofilms. The biofilms grown over the formulated materials were assessed by colony-forming unit (CFU) counting assay and fluorescence microscopy staining. In addition, the cytotoxicity was evaluated. Samples were subjected to 10,000 thermal cycles for aging and evaluated by UTS, µTBS, and CFU. Incorporating BDMDAC did not increase the cytotoxicity or change the physical properties when the mass fraction of the BDMDAC was 1–5 wt.%. The UTS of BDMDAC-doped adhesives was not impaired immediately or over time. A significant bacterial reduction was obtained for the mass fraction of the BDMDAC greater than 3 wt.%. However, the BDMDAC-doped adhesives did not offer an antibacterial effect after artificial aging. The overall results indicate that the BDMDAC strategy has the potential to control of microbial growth of cariogenic planktonic cells and biofilms. However, other new technological approaches are needed to overcome the deleterious effect of BDMDAC release over time such as those based on the principle of drug delivery systems whereby the BDMDAC is transported on microparticles or core shells, providing tangible benefits to oral health over time. MDPI 2022-10-17 /pmc/articles/PMC9624347/ /pubmed/36278659 http://dx.doi.org/10.3390/jfb13040190 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mokeem, Lamia Sami
Balhaddad, Abdulrahman A.
Garcia, Isadora Martini
Collares, Fabrício Mezzomo
Melo, Mary Anne S.
Benzyldimethyldodecyl Ammonium Chloride Doped Dental Adhesive: Impact on Core’s Properties, Biosafety, and Antibacterial/Bonding Performance after Aging
title Benzyldimethyldodecyl Ammonium Chloride Doped Dental Adhesive: Impact on Core’s Properties, Biosafety, and Antibacterial/Bonding Performance after Aging
title_full Benzyldimethyldodecyl Ammonium Chloride Doped Dental Adhesive: Impact on Core’s Properties, Biosafety, and Antibacterial/Bonding Performance after Aging
title_fullStr Benzyldimethyldodecyl Ammonium Chloride Doped Dental Adhesive: Impact on Core’s Properties, Biosafety, and Antibacterial/Bonding Performance after Aging
title_full_unstemmed Benzyldimethyldodecyl Ammonium Chloride Doped Dental Adhesive: Impact on Core’s Properties, Biosafety, and Antibacterial/Bonding Performance after Aging
title_short Benzyldimethyldodecyl Ammonium Chloride Doped Dental Adhesive: Impact on Core’s Properties, Biosafety, and Antibacterial/Bonding Performance after Aging
title_sort benzyldimethyldodecyl ammonium chloride doped dental adhesive: impact on core’s properties, biosafety, and antibacterial/bonding performance after aging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624347/
https://www.ncbi.nlm.nih.gov/pubmed/36278659
http://dx.doi.org/10.3390/jfb13040190
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