ODP248 The Effects of Insulin-like Growth Factor-binding Protein-3 on Metabolism During Short Term Fasting in Mice in Vivo

IGFBP-3 is the main abundant circulating IGFBP and plays an important role in the metabolic effect of IGF-1. We investigated the effects of IGFBP-3 on metabolism in short-term fasting and found that when IGFBP-3 was added to mice, serum triglyceride concentration were significantly enhanced. Fasting also significantly enhanced triglyceride concentration in liver and serum to the same degree as IGFBP-3. IGFBP-3 transfection suppressed the expression of ketogenesis and beta oxidation genes in liver after 24 h fasting. Also, they were shown significantly increase oflipolysis related protein expression in peripheral adipocyte and decrease the adipocyte size. Additionally, fat accumulation was more increased in the liver transfected with IGFBP-3 after fasting than controls. These in vivo findings demonstrate that IGFBP-3 has potent lipid metabolism and fasting-antagonizing capability and suggest a role for IGFBP-3 in the pathogenesis and homeostasis control of metabolism. Presentation: No date and time listed