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In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino

Dioscorea nipponica Makino is an optimal candidate to develop the diosgenin industry in North China. Due to its increasing demand in the medicine industry, it is urgent to apply new biotechnological tools to foster breeds with desirable traits and enhanced secondary metabolite production. The produc...

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Autores principales: Dang, Shangni, Gao, Runmei, Zhang, Yuqing, Feng, Yumei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626472/
https://www.ncbi.nlm.nih.gov/pubmed/36319819
http://dx.doi.org/10.1038/s41598-022-22986-4
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author Dang, Shangni
Gao, Runmei
Zhang, Yuqing
Feng, Yumei
author_facet Dang, Shangni
Gao, Runmei
Zhang, Yuqing
Feng, Yumei
author_sort Dang, Shangni
collection PubMed
description Dioscorea nipponica Makino is an optimal candidate to develop the diosgenin industry in North China. Due to its increasing demand in the medicine industry, it is urgent to apply new biotechnological tools to foster breeds with desirable traits and enhanced secondary metabolite production. The production of useful metabolites by the in vitro cultured rhizomes can be explored successfully for utilization by various food and drug industries. In this study, we reported callus formation and plantlet regeneration of the medicinal plant D. nipponica. Explants of leaves, stem segments and rhizomes of aseptic seedlings were cultured on Murashige and Skoog (MS) medium containing various combinations of auxin and cytokinin to find the optimal PGRs of each type of explant for callus induction and shoot regeneration of D. nipponica. The paraffin section technique was also used to observe of the morphogenesis of callus and adventitious bud. Explants of seeds and rhizomes formed calli at high frequency in all lines we examined. However, the explant of leaves rarely formed callus. Three kinds of callus were detected during the induction phase. Here, we describe three types of callus (Callus I–III) with different structure characteristics. Greenish in color and a nodule-like protrusion surface (Callus type III) were arranged more closely of cells with less interstitial substance, cell differentiation ability stronger than other callus types. The optimum combination was the maximum shoot differentiation frequency of 90% in callus derived from seeds cultured on MS medium with 2.0 mg L(−1)6-BA + 0.2 mg L(−1)NAA. The shoot differentiation frequency (88.57%) of rhizome-induced callus was obtained by the combination of MS medium supplemented with 3.0 mg L(−1)6-BA + 2.0 mg L(−1)NAA. 1/2 MS medium plus 0.5 mg L(−1)NAA resulted in a higher root regeneration frequency of 86.70%. In vitro propagated plantlets with healthy roots were domesticated and transplanted into small plastic pots containing sterile soil rite under greenhouse conditions with 80% survivability. Bud differentiation is mostly of exogenous origin, mostly occurring on the near callus surface. Therefore, it may be surmised that in vitro morphogenesis of D. nipponica is mainly caused by indirect organogenesis (adventitious bud).
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spelling pubmed-96264722022-11-03 In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino Dang, Shangni Gao, Runmei Zhang, Yuqing Feng, Yumei Sci Rep Article Dioscorea nipponica Makino is an optimal candidate to develop the diosgenin industry in North China. Due to its increasing demand in the medicine industry, it is urgent to apply new biotechnological tools to foster breeds with desirable traits and enhanced secondary metabolite production. The production of useful metabolites by the in vitro cultured rhizomes can be explored successfully for utilization by various food and drug industries. In this study, we reported callus formation and plantlet regeneration of the medicinal plant D. nipponica. Explants of leaves, stem segments and rhizomes of aseptic seedlings were cultured on Murashige and Skoog (MS) medium containing various combinations of auxin and cytokinin to find the optimal PGRs of each type of explant for callus induction and shoot regeneration of D. nipponica. The paraffin section technique was also used to observe of the morphogenesis of callus and adventitious bud. Explants of seeds and rhizomes formed calli at high frequency in all lines we examined. However, the explant of leaves rarely formed callus. Three kinds of callus were detected during the induction phase. Here, we describe three types of callus (Callus I–III) with different structure characteristics. Greenish in color and a nodule-like protrusion surface (Callus type III) were arranged more closely of cells with less interstitial substance, cell differentiation ability stronger than other callus types. The optimum combination was the maximum shoot differentiation frequency of 90% in callus derived from seeds cultured on MS medium with 2.0 mg L(−1)6-BA + 0.2 mg L(−1)NAA. The shoot differentiation frequency (88.57%) of rhizome-induced callus was obtained by the combination of MS medium supplemented with 3.0 mg L(−1)6-BA + 2.0 mg L(−1)NAA. 1/2 MS medium plus 0.5 mg L(−1)NAA resulted in a higher root regeneration frequency of 86.70%. In vitro propagated plantlets with healthy roots were domesticated and transplanted into small plastic pots containing sterile soil rite under greenhouse conditions with 80% survivability. Bud differentiation is mostly of exogenous origin, mostly occurring on the near callus surface. Therefore, it may be surmised that in vitro morphogenesis of D. nipponica is mainly caused by indirect organogenesis (adventitious bud). Nature Publishing Group UK 2022-11-01 /pmc/articles/PMC9626472/ /pubmed/36319819 http://dx.doi.org/10.1038/s41598-022-22986-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Dang, Shangni
Gao, Runmei
Zhang, Yuqing
Feng, Yumei
In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino
title In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino
title_full In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino
title_fullStr In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino
title_full_unstemmed In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino
title_short In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino
title_sort in vitro regeneration and its histological characteristics of dioscorea nipponica makino
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626472/
https://www.ncbi.nlm.nih.gov/pubmed/36319819
http://dx.doi.org/10.1038/s41598-022-22986-4
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