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An ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice
Currently, implants are utilized clinically for bone transplant procedures. However, if infectious osteomyelitis occurs at implant sites, removal of bacteria can be challenging. Moreover, altered blood flow at peri-implant infectious sites can create an anaerobic environment, making it more difficul...
Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626628/ https://www.ncbi.nlm.nih.gov/pubmed/36319854 http://dx.doi.org/10.1038/s41598-022-23322-6 |
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author | Soma, Tomoya Iwasaki, Ryotaro Sato, Yuiko Kobayashi, Tami Ito, Eri Matsumoto, Tatsuaki Kimura, Atsushi Homma, Fuka Saiki, Keitarou Takahashi, Yukihiro Miyamoto, Kana Matsumoto, Morio Nakamura, Masaya Morita, Mayu Ishii, Ken Asoda, Seiji Kawana, Hiromasa Xingyu, Zhu Aizawa, Mamoru Nakagawa, Taneaki Miyamoto, Takeshi |
author_facet | Soma, Tomoya Iwasaki, Ryotaro Sato, Yuiko Kobayashi, Tami Ito, Eri Matsumoto, Tatsuaki Kimura, Atsushi Homma, Fuka Saiki, Keitarou Takahashi, Yukihiro Miyamoto, Kana Matsumoto, Morio Nakamura, Masaya Morita, Mayu Ishii, Ken Asoda, Seiji Kawana, Hiromasa Xingyu, Zhu Aizawa, Mamoru Nakagawa, Taneaki Miyamoto, Takeshi |
author_sort | Soma, Tomoya |
collection | PubMed |
description | Currently, implants are utilized clinically for bone transplant procedures. However, if infectious osteomyelitis occurs at implant sites, removal of bacteria can be challenging. Moreover, altered blood flow at peri-implant infectious sites can create an anaerobic environment, making it more difficult to treat infection with antibiotics. Thus, it would be beneficial if implants could be modified to exhibit antibacterial activity, even in anaerobic conditions. Here, we show antibacterial activity of silver ions coated on titanium rods, even against the anaerobic bacteria Porphyromonas gingivalis (P. gingivalis), both in vitro and in vivo. Specifically, we implanted silver-coated or control uncoated titanium rods along with P. gingivalis in mouse femoral bone BM cavities and observed significantly inhibited P. gingivalis infection with silver-coated compared with non-coated rods, based on in vivo bio-imaging. Osteonecrosis by infectious osteomyelitis and elevation of the inflammatory factors C-reactive protein and IL-6 promoted by P. gingivalis s were also significantly reduced in the presence of silver-coated rods. Overall, our study indicates that silver ion coating of an implant represents a therapeutic option to prevent associated infection, even in anaerobic conditions or against anaerobic bacteria. |
format | Online Article Text |
id | pubmed-9626628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-96266282022-11-03 An ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice Soma, Tomoya Iwasaki, Ryotaro Sato, Yuiko Kobayashi, Tami Ito, Eri Matsumoto, Tatsuaki Kimura, Atsushi Homma, Fuka Saiki, Keitarou Takahashi, Yukihiro Miyamoto, Kana Matsumoto, Morio Nakamura, Masaya Morita, Mayu Ishii, Ken Asoda, Seiji Kawana, Hiromasa Xingyu, Zhu Aizawa, Mamoru Nakagawa, Taneaki Miyamoto, Takeshi Sci Rep Article Currently, implants are utilized clinically for bone transplant procedures. However, if infectious osteomyelitis occurs at implant sites, removal of bacteria can be challenging. Moreover, altered blood flow at peri-implant infectious sites can create an anaerobic environment, making it more difficult to treat infection with antibiotics. Thus, it would be beneficial if implants could be modified to exhibit antibacterial activity, even in anaerobic conditions. Here, we show antibacterial activity of silver ions coated on titanium rods, even against the anaerobic bacteria Porphyromonas gingivalis (P. gingivalis), both in vitro and in vivo. Specifically, we implanted silver-coated or control uncoated titanium rods along with P. gingivalis in mouse femoral bone BM cavities and observed significantly inhibited P. gingivalis infection with silver-coated compared with non-coated rods, based on in vivo bio-imaging. Osteonecrosis by infectious osteomyelitis and elevation of the inflammatory factors C-reactive protein and IL-6 promoted by P. gingivalis s were also significantly reduced in the presence of silver-coated rods. Overall, our study indicates that silver ion coating of an implant represents a therapeutic option to prevent associated infection, even in anaerobic conditions or against anaerobic bacteria. Nature Publishing Group UK 2022-11-01 /pmc/articles/PMC9626628/ /pubmed/36319854 http://dx.doi.org/10.1038/s41598-022-23322-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Soma, Tomoya Iwasaki, Ryotaro Sato, Yuiko Kobayashi, Tami Ito, Eri Matsumoto, Tatsuaki Kimura, Atsushi Homma, Fuka Saiki, Keitarou Takahashi, Yukihiro Miyamoto, Kana Matsumoto, Morio Nakamura, Masaya Morita, Mayu Ishii, Ken Asoda, Seiji Kawana, Hiromasa Xingyu, Zhu Aizawa, Mamoru Nakagawa, Taneaki Miyamoto, Takeshi An ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice |
title | An ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice |
title_full | An ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice |
title_fullStr | An ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice |
title_full_unstemmed | An ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice |
title_short | An ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice |
title_sort | ionic silver coating prevents implant-associated infection by anaerobic bacteria in vitro and in vivo in mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626628/ https://www.ncbi.nlm.nih.gov/pubmed/36319854 http://dx.doi.org/10.1038/s41598-022-23322-6 |
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