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Serum extracellular traps associate with the activation of myeloid cells in SLE patients with the low level of anti-DNA antibodies

Neutrophil extracellular traps (NETs) are involved in systemic lupus erythematosus (SLE). We sought to cluster SLE patients based on serum NET levels. Serum NET levels were higher in SLE patients than healthy controls. Frequencies of pleuritis and myositis were increased in patients with high serum...

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Autores principales: Hanata, Norio, Ota, Mineto, Tsuchida, Yumi, Nagafuchi, Yasuo, Okamura, Tomohisa, Shoda, Hirofumi, Fujio, Keishi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626644/
https://www.ncbi.nlm.nih.gov/pubmed/36319843
http://dx.doi.org/10.1038/s41598-022-23076-1
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author Hanata, Norio
Ota, Mineto
Tsuchida, Yumi
Nagafuchi, Yasuo
Okamura, Tomohisa
Shoda, Hirofumi
Fujio, Keishi
author_facet Hanata, Norio
Ota, Mineto
Tsuchida, Yumi
Nagafuchi, Yasuo
Okamura, Tomohisa
Shoda, Hirofumi
Fujio, Keishi
author_sort Hanata, Norio
collection PubMed
description Neutrophil extracellular traps (NETs) are involved in systemic lupus erythematosus (SLE). We sought to cluster SLE patients based on serum NET levels. Serum NET levels were higher in SLE patients than healthy controls. Frequencies of pleuritis and myositis were increased in patients with high serum NET levels. Serum NET levels negatively correlated with anti–double stranded DNA (anti-dsDNA) antibody titers and C1q-binding immune complexes, but positively correlated with C-reactive protein (CRP) and monocyte counts. Neutrophil transcriptome analysis demonstrated no difference in NET-associated signatures, irrespective of serum NET levels, suggesting anti-dsDNA antibody-mediated clearance of NETs. In serum, NET levels were significantly correlated with myeloid cell-derived inflammatory molecules. Serum NET-based cluster analysis revealed 3 groups of patients based on serum NET and CRP levels, anti-dsDNA antibody titers, and monocyte count. Monocytes were consistently activated following NET-containing immune complex (NET-IC) stimulation. In conclusion, SLE patients with high serum NET levels had lower anti-dsDNA antibody titers and higher inflammatory responses. NET-IC-stimulated monocytes might associate with an inflammatory response characterized by elevated CRP levels. These findings can apply to precision medicine, as inflammatory processes, rather than antibody-dependent processes, can be targeted in specific subpopulations of SLE patients.
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spelling pubmed-96266442022-11-02 Serum extracellular traps associate with the activation of myeloid cells in SLE patients with the low level of anti-DNA antibodies Hanata, Norio Ota, Mineto Tsuchida, Yumi Nagafuchi, Yasuo Okamura, Tomohisa Shoda, Hirofumi Fujio, Keishi Sci Rep Article Neutrophil extracellular traps (NETs) are involved in systemic lupus erythematosus (SLE). We sought to cluster SLE patients based on serum NET levels. Serum NET levels were higher in SLE patients than healthy controls. Frequencies of pleuritis and myositis were increased in patients with high serum NET levels. Serum NET levels negatively correlated with anti–double stranded DNA (anti-dsDNA) antibody titers and C1q-binding immune complexes, but positively correlated with C-reactive protein (CRP) and monocyte counts. Neutrophil transcriptome analysis demonstrated no difference in NET-associated signatures, irrespective of serum NET levels, suggesting anti-dsDNA antibody-mediated clearance of NETs. In serum, NET levels were significantly correlated with myeloid cell-derived inflammatory molecules. Serum NET-based cluster analysis revealed 3 groups of patients based on serum NET and CRP levels, anti-dsDNA antibody titers, and monocyte count. Monocytes were consistently activated following NET-containing immune complex (NET-IC) stimulation. In conclusion, SLE patients with high serum NET levels had lower anti-dsDNA antibody titers and higher inflammatory responses. NET-IC-stimulated monocytes might associate with an inflammatory response characterized by elevated CRP levels. These findings can apply to precision medicine, as inflammatory processes, rather than antibody-dependent processes, can be targeted in specific subpopulations of SLE patients. Nature Publishing Group UK 2022-11-01 /pmc/articles/PMC9626644/ /pubmed/36319843 http://dx.doi.org/10.1038/s41598-022-23076-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hanata, Norio
Ota, Mineto
Tsuchida, Yumi
Nagafuchi, Yasuo
Okamura, Tomohisa
Shoda, Hirofumi
Fujio, Keishi
Serum extracellular traps associate with the activation of myeloid cells in SLE patients with the low level of anti-DNA antibodies
title Serum extracellular traps associate with the activation of myeloid cells in SLE patients with the low level of anti-DNA antibodies
title_full Serum extracellular traps associate with the activation of myeloid cells in SLE patients with the low level of anti-DNA antibodies
title_fullStr Serum extracellular traps associate with the activation of myeloid cells in SLE patients with the low level of anti-DNA antibodies
title_full_unstemmed Serum extracellular traps associate with the activation of myeloid cells in SLE patients with the low level of anti-DNA antibodies
title_short Serum extracellular traps associate with the activation of myeloid cells in SLE patients with the low level of anti-DNA antibodies
title_sort serum extracellular traps associate with the activation of myeloid cells in sle patients with the low level of anti-dna antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626644/
https://www.ncbi.nlm.nih.gov/pubmed/36319843
http://dx.doi.org/10.1038/s41598-022-23076-1
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