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Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart

Aims: Gross pathology inspection (patho) is the gold standard for the morphological evaluation of focal myocardial pathology. Examination with 9.4 T magnetic resonance imaging (MRI) is a new method for very accurate display of myocardial pathology. The aim of this study was to demonstrate that lesio...

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Autores principales: Odehnalová, Eva, Valíková, Lucia, Caluori, Guido, Kulík, Tomáš, Římalová, Veronika, Jadczyk, Tomasz, Dražanová, Eva, Pavlova, Iveta, Pešl, Martin, Kubeš, Václav, Stárek, Zdeněk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626654/
https://www.ncbi.nlm.nih.gov/pubmed/36338496
http://dx.doi.org/10.3389/fphys.2022.834328
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author Odehnalová, Eva
Valíková, Lucia
Caluori, Guido
Kulík, Tomáš
Římalová, Veronika
Jadczyk, Tomasz
Dražanová, Eva
Pavlova, Iveta
Pešl, Martin
Kubeš, Václav
Stárek, Zdeněk
author_facet Odehnalová, Eva
Valíková, Lucia
Caluori, Guido
Kulík, Tomáš
Římalová, Veronika
Jadczyk, Tomasz
Dražanová, Eva
Pavlova, Iveta
Pešl, Martin
Kubeš, Václav
Stárek, Zdeněk
author_sort Odehnalová, Eva
collection PubMed
description Aims: Gross pathology inspection (patho) is the gold standard for the morphological evaluation of focal myocardial pathology. Examination with 9.4 T magnetic resonance imaging (MRI) is a new method for very accurate display of myocardial pathology. The aim of this study was to demonstrate that lesions can be measured on high-resolution MRI images with the same accuracy as on pathological sections and compare these two methods for the evaluation of radiofrequency (RF) ablation lesion dimensions in swine heart tissue during animal experiment. Methods: Ten pigs underwent radiofrequency ablations in the left ventricle during animal experiment. After animal euthanasia, hearts were explanted, flushed with ice-cold cardioplegic solution to relax the whole myocardium, fixed in 10% formaldehyde and scanned with a 9.4 T magnetic resonance system. Anatomical images were processed using ImageJ software. Subsequently, the hearts were sliced, slices were photographed and measured in ImageJ software. Different dimensions and volumes were compared. Results: The results of both methods were statistically compared. Depth by MRI was 8.771 ± 2.595 mm and by patho 9.008 ± 2.823 mm; p = 0.198. Width was 10.802 ± 2.724 mm by MRI and 11.125 ± 2.801 mm by patho; p = 0.049. Estuary was 2.006 ± 0.867 mm by MRI and 2.001 ± 0.872 mm by patho; p = 0.953. The depth at the maximum diameter was 4.734 ± 1.532 mm on MRI and 4.783 ± 1.648 mm from the patho; p = 0.858. The volumes of the lesions calculated using a formula were 315.973 ± 257.673 mm(3) for MRI and 355.726 ± 255.860 mm(3) for patho; p = 0.104. Volume directly measured from MRI with the “point-by-point” method was 671.702 ± 362.299 mm(3). Conclusion: Measurements obtained from gross pathology inspection and MRI are fully comparable. The advantage of MRI is that it is a non-destructive method enabling repeated measurements in all possible anatomical projections.
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spelling pubmed-96266542022-11-03 Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart Odehnalová, Eva Valíková, Lucia Caluori, Guido Kulík, Tomáš Římalová, Veronika Jadczyk, Tomasz Dražanová, Eva Pavlova, Iveta Pešl, Martin Kubeš, Václav Stárek, Zdeněk Front Physiol Physiology Aims: Gross pathology inspection (patho) is the gold standard for the morphological evaluation of focal myocardial pathology. Examination with 9.4 T magnetic resonance imaging (MRI) is a new method for very accurate display of myocardial pathology. The aim of this study was to demonstrate that lesions can be measured on high-resolution MRI images with the same accuracy as on pathological sections and compare these two methods for the evaluation of radiofrequency (RF) ablation lesion dimensions in swine heart tissue during animal experiment. Methods: Ten pigs underwent radiofrequency ablations in the left ventricle during animal experiment. After animal euthanasia, hearts were explanted, flushed with ice-cold cardioplegic solution to relax the whole myocardium, fixed in 10% formaldehyde and scanned with a 9.4 T magnetic resonance system. Anatomical images were processed using ImageJ software. Subsequently, the hearts were sliced, slices were photographed and measured in ImageJ software. Different dimensions and volumes were compared. Results: The results of both methods were statistically compared. Depth by MRI was 8.771 ± 2.595 mm and by patho 9.008 ± 2.823 mm; p = 0.198. Width was 10.802 ± 2.724 mm by MRI and 11.125 ± 2.801 mm by patho; p = 0.049. Estuary was 2.006 ± 0.867 mm by MRI and 2.001 ± 0.872 mm by patho; p = 0.953. The depth at the maximum diameter was 4.734 ± 1.532 mm on MRI and 4.783 ± 1.648 mm from the patho; p = 0.858. The volumes of the lesions calculated using a formula were 315.973 ± 257.673 mm(3) for MRI and 355.726 ± 255.860 mm(3) for patho; p = 0.104. Volume directly measured from MRI with the “point-by-point” method was 671.702 ± 362.299 mm(3). Conclusion: Measurements obtained from gross pathology inspection and MRI are fully comparable. The advantage of MRI is that it is a non-destructive method enabling repeated measurements in all possible anatomical projections. Frontiers Media S.A. 2022-10-19 /pmc/articles/PMC9626654/ /pubmed/36338496 http://dx.doi.org/10.3389/fphys.2022.834328 Text en Copyright © 2022 Odehnalová, Valíková, Caluori, Kulík, Římalová, Jadczyk, Dražanová, Pavlova, Pešl, Kubeš and Stárek. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Odehnalová, Eva
Valíková, Lucia
Caluori, Guido
Kulík, Tomáš
Římalová, Veronika
Jadczyk, Tomasz
Dražanová, Eva
Pavlova, Iveta
Pešl, Martin
Kubeš, Václav
Stárek, Zdeněk
Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart
title Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart
title_full Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart
title_fullStr Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart
title_full_unstemmed Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart
title_short Comparison of gross pathology inspection and 9.4 T magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart
title_sort comparison of gross pathology inspection and 9.4 t magnetic resonance imaging in the evaluation of radiofrequency ablation lesions in the left ventricle of the swine heart
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626654/
https://www.ncbi.nlm.nih.gov/pubmed/36338496
http://dx.doi.org/10.3389/fphys.2022.834328
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