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Feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization

BACKGROUND: This research aims to evaluate the feasibility of using avian immunoglobulins (IgY) raised against adhesion factors of enterotoxigenic Escherichia coli (ETEC) as prophylaxis of diarrheal illness caused by these pathogens. ETEC requires adhesion to human intestinal epithelial cells as a p...

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Autores principales: Brumfield, Kyle, Seo, Hyesuk, Idegwu, Nnebuefe, Artman, Chad, Gonyar, Laura, Nataro, James, Zhang, Weiping, Sack, David, Geyer, James, Goepp, Julius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627289/
https://www.ncbi.nlm.nih.gov/pubmed/36341430
http://dx.doi.org/10.3389/fimmu.2022.1011200
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author Brumfield, Kyle
Seo, Hyesuk
Idegwu, Nnebuefe
Artman, Chad
Gonyar, Laura
Nataro, James
Zhang, Weiping
Sack, David
Geyer, James
Goepp, Julius
author_facet Brumfield, Kyle
Seo, Hyesuk
Idegwu, Nnebuefe
Artman, Chad
Gonyar, Laura
Nataro, James
Zhang, Weiping
Sack, David
Geyer, James
Goepp, Julius
author_sort Brumfield, Kyle
collection PubMed
description BACKGROUND: This research aims to evaluate the feasibility of using avian immunoglobulins (IgY) raised against adhesion factors of enterotoxigenic Escherichia coli (ETEC) as prophylaxis of diarrheal illness caused by these pathogens. ETEC requires adhesion to human intestinal epithelial cells as a primary step in establishing enteric infection. Therefore, inhibition of adhesion may prevent such infections and reduce clinical burdens of diarrheal illness. METHODS: IgY samples were prepared from eggs of hens immunized with an adhesin-tip multiepitope fusion antigen (MEFA), developed against nine adhesin tip epitopes derived from clinically relevant ETEC strains. The resulting IgY was evaluated for its ability to inhibit adhesion of ETEC to cell-surface targets. Potential impacts of anti-MEFA IgY on growth of both pathogenic and commensal E. coli isolates were also evaluated. RESULTS: Enzyme linked immunosorbent assay (ELISA) titers were achieved for IgY targeting each of the nine individual epitopes included in the adhesin-tip MEFA. Furthermore, anti-MEFA titers exceeding 1:2(19) were sustained for at least 23 weeks. All ETEC strains used in design of the adhesin-tip MEFA, and five of five clinical ETEC strains were significantly (P < 0.05) inhibited from adhesion to mammalian cells in culture. CONCLUSIONS: These findings demonstrate that IgY targeting ETEC adhesin-tip MEFA have the potential to disrupt in vitro adherence of ETEC. A formulation containing adhesin-tip MEFA IgY can be considered a potential candidate for in vivo evaluation as prophylaxis of diarrheal diseases. Animal studies of this formulation are planned.
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spelling pubmed-96272892022-11-03 Feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization Brumfield, Kyle Seo, Hyesuk Idegwu, Nnebuefe Artman, Chad Gonyar, Laura Nataro, James Zhang, Weiping Sack, David Geyer, James Goepp, Julius Front Immunol Immunology BACKGROUND: This research aims to evaluate the feasibility of using avian immunoglobulins (IgY) raised against adhesion factors of enterotoxigenic Escherichia coli (ETEC) as prophylaxis of diarrheal illness caused by these pathogens. ETEC requires adhesion to human intestinal epithelial cells as a primary step in establishing enteric infection. Therefore, inhibition of adhesion may prevent such infections and reduce clinical burdens of diarrheal illness. METHODS: IgY samples were prepared from eggs of hens immunized with an adhesin-tip multiepitope fusion antigen (MEFA), developed against nine adhesin tip epitopes derived from clinically relevant ETEC strains. The resulting IgY was evaluated for its ability to inhibit adhesion of ETEC to cell-surface targets. Potential impacts of anti-MEFA IgY on growth of both pathogenic and commensal E. coli isolates were also evaluated. RESULTS: Enzyme linked immunosorbent assay (ELISA) titers were achieved for IgY targeting each of the nine individual epitopes included in the adhesin-tip MEFA. Furthermore, anti-MEFA titers exceeding 1:2(19) were sustained for at least 23 weeks. All ETEC strains used in design of the adhesin-tip MEFA, and five of five clinical ETEC strains were significantly (P < 0.05) inhibited from adhesion to mammalian cells in culture. CONCLUSIONS: These findings demonstrate that IgY targeting ETEC adhesin-tip MEFA have the potential to disrupt in vitro adherence of ETEC. A formulation containing adhesin-tip MEFA IgY can be considered a potential candidate for in vivo evaluation as prophylaxis of diarrheal diseases. Animal studies of this formulation are planned. Frontiers Media S.A. 2022-10-19 /pmc/articles/PMC9627289/ /pubmed/36341430 http://dx.doi.org/10.3389/fimmu.2022.1011200 Text en Copyright © 2022 Brumfield, Seo, Idegwu, Artman, Gonyar, Nataro, Zhang, Sack, Geyer and Goepp https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Brumfield, Kyle
Seo, Hyesuk
Idegwu, Nnebuefe
Artman, Chad
Gonyar, Laura
Nataro, James
Zhang, Weiping
Sack, David
Geyer, James
Goepp, Julius
Feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization
title Feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization
title_full Feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization
title_fullStr Feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization
title_full_unstemmed Feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization
title_short Feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization
title_sort feasibility of avian antibodies as prophylaxis against enterotoxigenic escherichia coli colonization
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627289/
https://www.ncbi.nlm.nih.gov/pubmed/36341430
http://dx.doi.org/10.3389/fimmu.2022.1011200
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