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Driving down malaria transmission with engineered gene drives

The last century has witnessed the introduction, establishment and expansion of mosquito-borne diseases into diverse new geographic ranges. Malaria is transmitted by female Anopheles mosquitoes. Despite making great strides over the past few decades in reducing the burden of malaria, transmission is...

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Autores principales: Garrood, William T., Cuber, Piotr, Willis, Katie, Bernardini, Federica, Page, Nicole M., Haghighat-Khah, Roya E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627344/
https://www.ncbi.nlm.nih.gov/pubmed/36338968
http://dx.doi.org/10.3389/fgene.2022.891218
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author Garrood, William T.
Cuber, Piotr
Willis, Katie
Bernardini, Federica
Page, Nicole M.
Haghighat-Khah, Roya E.
author_facet Garrood, William T.
Cuber, Piotr
Willis, Katie
Bernardini, Federica
Page, Nicole M.
Haghighat-Khah, Roya E.
author_sort Garrood, William T.
collection PubMed
description The last century has witnessed the introduction, establishment and expansion of mosquito-borne diseases into diverse new geographic ranges. Malaria is transmitted by female Anopheles mosquitoes. Despite making great strides over the past few decades in reducing the burden of malaria, transmission is now on the rise again, in part owing to the emergence of mosquito resistance to insecticides, antimalarial drug resistance and, more recently, the challenges of the COVID-19 pandemic, which resulted in the reduced implementation efficiency of various control programs. The utility of genetically engineered gene drive mosquitoes as tools to decrease the burden of malaria by controlling the disease-transmitting mosquitoes is being evaluated. To date, there has been remarkable progress in the development of CRISPR/Cas9-based homing endonuclease designs in malaria mosquitoes due to successful proof-of-principle and multigenerational experiments. In this review, we examine the lessons learnt from the development of current CRISPR/Cas9-based homing endonuclease gene drives, providing a framework for the development of gene drive systems for the targeted control of wild malaria-transmitting mosquito populations that overcome challenges such as with evolving drive-resistance. We also discuss the additional substantial works required to progress the development of gene drive systems from scientific discovery to further study and subsequent field application in endemic settings.
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spelling pubmed-96273442022-11-03 Driving down malaria transmission with engineered gene drives Garrood, William T. Cuber, Piotr Willis, Katie Bernardini, Federica Page, Nicole M. Haghighat-Khah, Roya E. Front Genet Genetics The last century has witnessed the introduction, establishment and expansion of mosquito-borne diseases into diverse new geographic ranges. Malaria is transmitted by female Anopheles mosquitoes. Despite making great strides over the past few decades in reducing the burden of malaria, transmission is now on the rise again, in part owing to the emergence of mosquito resistance to insecticides, antimalarial drug resistance and, more recently, the challenges of the COVID-19 pandemic, which resulted in the reduced implementation efficiency of various control programs. The utility of genetically engineered gene drive mosquitoes as tools to decrease the burden of malaria by controlling the disease-transmitting mosquitoes is being evaluated. To date, there has been remarkable progress in the development of CRISPR/Cas9-based homing endonuclease designs in malaria mosquitoes due to successful proof-of-principle and multigenerational experiments. In this review, we examine the lessons learnt from the development of current CRISPR/Cas9-based homing endonuclease gene drives, providing a framework for the development of gene drive systems for the targeted control of wild malaria-transmitting mosquito populations that overcome challenges such as with evolving drive-resistance. We also discuss the additional substantial works required to progress the development of gene drive systems from scientific discovery to further study and subsequent field application in endemic settings. Frontiers Media S.A. 2022-10-19 /pmc/articles/PMC9627344/ /pubmed/36338968 http://dx.doi.org/10.3389/fgene.2022.891218 Text en Copyright © 2022 Garrood, Cuber, Willis, Bernardini, Page and Haghighat-Khah. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Garrood, William T.
Cuber, Piotr
Willis, Katie
Bernardini, Federica
Page, Nicole M.
Haghighat-Khah, Roya E.
Driving down malaria transmission with engineered gene drives
title Driving down malaria transmission with engineered gene drives
title_full Driving down malaria transmission with engineered gene drives
title_fullStr Driving down malaria transmission with engineered gene drives
title_full_unstemmed Driving down malaria transmission with engineered gene drives
title_short Driving down malaria transmission with engineered gene drives
title_sort driving down malaria transmission with engineered gene drives
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627344/
https://www.ncbi.nlm.nih.gov/pubmed/36338968
http://dx.doi.org/10.3389/fgene.2022.891218
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