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Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes
The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were us...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627484/ https://www.ncbi.nlm.nih.gov/pubmed/36340347 http://dx.doi.org/10.3389/fpls.2022.988594 |
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author | Zhou, Peina Chen, Hongyu Dang, Jingjie Shi, Zunrui Shao, Yongfang Liu, Chanchan Fan, Longjiang Wu, Qinan |
author_facet | Zhou, Peina Chen, Hongyu Dang, Jingjie Shi, Zunrui Shao, Yongfang Liu, Chanchan Fan, Longjiang Wu, Qinan |
author_sort | Zhou, Peina |
collection | PubMed |
description | The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were used to isolated protoplasts and successfully captured 33,254 protoplasts for sequencing purposes. After cell type annotation, all the cells were partitioned into six broad populations with 19 clusters. Cells from PGTs were identified based on the expression patterns of trichome-specific genes, monoterpene biosynthetic genes, and metabolic analysis of PGT secretions. The developmental trajectories of PGTs were delineated by pseudotime analysis. Integrative analysis of scRNA-seq data from N. tenuifolia leaves and Arabidopsis thaliana shoot revealed that PGTs were specific to N. tenuifolia. Thus, our results provide a promising basis for exploring cell development and differentiation in plants, especially glandular trichome initiation and development. |
format | Online Article Text |
id | pubmed-9627484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-96274842022-11-03 Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes Zhou, Peina Chen, Hongyu Dang, Jingjie Shi, Zunrui Shao, Yongfang Liu, Chanchan Fan, Longjiang Wu, Qinan Front Plant Sci Plant Science The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were used to isolated protoplasts and successfully captured 33,254 protoplasts for sequencing purposes. After cell type annotation, all the cells were partitioned into six broad populations with 19 clusters. Cells from PGTs were identified based on the expression patterns of trichome-specific genes, monoterpene biosynthetic genes, and metabolic analysis of PGT secretions. The developmental trajectories of PGTs were delineated by pseudotime analysis. Integrative analysis of scRNA-seq data from N. tenuifolia leaves and Arabidopsis thaliana shoot revealed that PGTs were specific to N. tenuifolia. Thus, our results provide a promising basis for exploring cell development and differentiation in plants, especially glandular trichome initiation and development. Frontiers Media S.A. 2022-10-19 /pmc/articles/PMC9627484/ /pubmed/36340347 http://dx.doi.org/10.3389/fpls.2022.988594 Text en Copyright © 2022 Zhou, Chen, Dang, Shi, Shao, Liu, Fan and Wu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhou, Peina Chen, Hongyu Dang, Jingjie Shi, Zunrui Shao, Yongfang Liu, Chanchan Fan, Longjiang Wu, Qinan Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes |
title | Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes |
title_full | Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes |
title_fullStr | Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes |
title_full_unstemmed | Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes |
title_short | Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes |
title_sort | single-cell transcriptome of nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627484/ https://www.ncbi.nlm.nih.gov/pubmed/36340347 http://dx.doi.org/10.3389/fpls.2022.988594 |
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