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Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes

The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were us...

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Autores principales: Zhou, Peina, Chen, Hongyu, Dang, Jingjie, Shi, Zunrui, Shao, Yongfang, Liu, Chanchan, Fan, Longjiang, Wu, Qinan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627484/
https://www.ncbi.nlm.nih.gov/pubmed/36340347
http://dx.doi.org/10.3389/fpls.2022.988594
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author Zhou, Peina
Chen, Hongyu
Dang, Jingjie
Shi, Zunrui
Shao, Yongfang
Liu, Chanchan
Fan, Longjiang
Wu, Qinan
author_facet Zhou, Peina
Chen, Hongyu
Dang, Jingjie
Shi, Zunrui
Shao, Yongfang
Liu, Chanchan
Fan, Longjiang
Wu, Qinan
author_sort Zhou, Peina
collection PubMed
description The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were used to isolated protoplasts and successfully captured 33,254 protoplasts for sequencing purposes. After cell type annotation, all the cells were partitioned into six broad populations with 19 clusters. Cells from PGTs were identified based on the expression patterns of trichome-specific genes, monoterpene biosynthetic genes, and metabolic analysis of PGT secretions. The developmental trajectories of PGTs were delineated by pseudotime analysis. Integrative analysis of scRNA-seq data from N. tenuifolia leaves and Arabidopsis thaliana shoot revealed that PGTs were specific to N. tenuifolia. Thus, our results provide a promising basis for exploring cell development and differentiation in plants, especially glandular trichome initiation and development.
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spelling pubmed-96274842022-11-03 Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes Zhou, Peina Chen, Hongyu Dang, Jingjie Shi, Zunrui Shao, Yongfang Liu, Chanchan Fan, Longjiang Wu, Qinan Front Plant Sci Plant Science The peltate glandular trichomes (PGTs) on Nepeta tenuifolia leaves can secrete and store bioactive essential oils. ScRNA-seq is a powerful tool for uncovering heterogeneous cells and exploring the development and differentiation of specific cells. Due to leaves rich in PGTs, the young leaves were used to isolated protoplasts and successfully captured 33,254 protoplasts for sequencing purposes. After cell type annotation, all the cells were partitioned into six broad populations with 19 clusters. Cells from PGTs were identified based on the expression patterns of trichome-specific genes, monoterpene biosynthetic genes, and metabolic analysis of PGT secretions. The developmental trajectories of PGTs were delineated by pseudotime analysis. Integrative analysis of scRNA-seq data from N. tenuifolia leaves and Arabidopsis thaliana shoot revealed that PGTs were specific to N. tenuifolia. Thus, our results provide a promising basis for exploring cell development and differentiation in plants, especially glandular trichome initiation and development. Frontiers Media S.A. 2022-10-19 /pmc/articles/PMC9627484/ /pubmed/36340347 http://dx.doi.org/10.3389/fpls.2022.988594 Text en Copyright © 2022 Zhou, Chen, Dang, Shi, Shao, Liu, Fan and Wu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhou, Peina
Chen, Hongyu
Dang, Jingjie
Shi, Zunrui
Shao, Yongfang
Liu, Chanchan
Fan, Longjiang
Wu, Qinan
Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes
title Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes
title_full Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes
title_fullStr Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes
title_full_unstemmed Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes
title_short Single-cell transcriptome of Nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes
title_sort single-cell transcriptome of nepeta tenuifolia leaves reveal differentiation trajectories in glandular trichomes
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627484/
https://www.ncbi.nlm.nih.gov/pubmed/36340347
http://dx.doi.org/10.3389/fpls.2022.988594
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