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Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B

Most of WRKY transcription factors play important roles in plant development, protection against disease, and response to abiotic stress; however, their roles in lily are largely unknown. Transcriptome analysis in lily (Lilium longiflorum) led to the identification and isolation of a WRKY-IIe gene,...

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Autores principales: Wu, Ze, Li, Ting, Cao, Xing, Zhang, Dehua, Teng, Nianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627522/
https://www.ncbi.nlm.nih.gov/pubmed/36338843
http://dx.doi.org/10.1093/hr/uhac186
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author Wu, Ze
Li, Ting
Cao, Xing
Zhang, Dehua
Teng, Nianjun
author_facet Wu, Ze
Li, Ting
Cao, Xing
Zhang, Dehua
Teng, Nianjun
author_sort Wu, Ze
collection PubMed
description Most of WRKY transcription factors play important roles in plant development, protection against disease, and response to abiotic stress; however, their roles in lily are largely unknown. Transcriptome analysis in lily (Lilium longiflorum) led to the identification and isolation of a WRKY-IIe gene, LlWRKY22, which was found to be activated at high temperature and play a positive role in thermotolerance regulation. LlWRKY22 expression was continuously activated by heat stress. We further found that LlWRKY22 protein localized to the nucleus and exhibited transactivation activity in both yeast and plant cells, and that its C terminus contributed to its transactivation activity. Meanwhile, overexpression of LlWRKY22 in lily improved thermotolerance and activated the expression of heat-related LlDREB2B gene; however, silencing of LlWRKY22 exerted the opposite effects. Further analysis revealed that LlWRKY22 directly activated the expression of LlDREB2B by binding to two tandem W-box elements on its promoter. Simultaneously, we also found that LlWRKY22 can directly bind its own promoter, thereby activating its own expression and forming a positive regulatory loop. Combined, our findings demonstrated that LlWRKY22 may be a new regulator of heat stress response and positively participates in the establishment of thermotolerance by activating itself and LlDREB2B.
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spelling pubmed-96275222022-11-04 Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B Wu, Ze Li, Ting Cao, Xing Zhang, Dehua Teng, Nianjun Hortic Res Article Most of WRKY transcription factors play important roles in plant development, protection against disease, and response to abiotic stress; however, their roles in lily are largely unknown. Transcriptome analysis in lily (Lilium longiflorum) led to the identification and isolation of a WRKY-IIe gene, LlWRKY22, which was found to be activated at high temperature and play a positive role in thermotolerance regulation. LlWRKY22 expression was continuously activated by heat stress. We further found that LlWRKY22 protein localized to the nucleus and exhibited transactivation activity in both yeast and plant cells, and that its C terminus contributed to its transactivation activity. Meanwhile, overexpression of LlWRKY22 in lily improved thermotolerance and activated the expression of heat-related LlDREB2B gene; however, silencing of LlWRKY22 exerted the opposite effects. Further analysis revealed that LlWRKY22 directly activated the expression of LlDREB2B by binding to two tandem W-box elements on its promoter. Simultaneously, we also found that LlWRKY22 can directly bind its own promoter, thereby activating its own expression and forming a positive regulatory loop. Combined, our findings demonstrated that LlWRKY22 may be a new regulator of heat stress response and positively participates in the establishment of thermotolerance by activating itself and LlDREB2B. Oxford University Press 2022-08-25 /pmc/articles/PMC9627522/ /pubmed/36338843 http://dx.doi.org/10.1093/hr/uhac186 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Wu, Ze
Li, Ting
Cao, Xing
Zhang, Dehua
Teng, Nianjun
Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B
title Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B
title_full Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B
title_fullStr Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B
title_full_unstemmed Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B
title_short Lily WRKY factor LlWRKY22 promotes thermotolerance through autoactivation and activation of LlDREB2B
title_sort lily wrky factor llwrky22 promotes thermotolerance through autoactivation and activation of lldreb2b
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627522/
https://www.ncbi.nlm.nih.gov/pubmed/36338843
http://dx.doi.org/10.1093/hr/uhac186
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