Biosensor Cell-Fit-HD(4D) for correlation of single-cell fate and microscale energy deposition in complex ion beams

We present a protocol for the biosensor Cell-Fit-HD(4D). It enables long-term monitoring and correlation of single-cell fate with subcellular-deposited energy of ionizing radiation. Cell fate tracking using widefield time-lapse microscopy is uncoupled in time from confocal ion track imaging. Registr...

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Detalles Bibliográficos
Autores principales: Schlegel, Julian, Liew, Hans, Rein, Katrin, Dzyubachyk, Oleh, Debus, Jürgen, Abdollahi, Amir, Niklas, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9627659/
https://www.ncbi.nlm.nih.gov/pubmed/36340882
http://dx.doi.org/10.1016/j.xpro.2022.101798
Descripción
Sumario:We present a protocol for the biosensor Cell-Fit-HD(4D). It enables long-term monitoring and correlation of single-cell fate with subcellular-deposited energy of ionizing radiation. Cell fate tracking using widefield time-lapse microscopy is uncoupled in time from confocal ion track imaging. Registration of both image acquisition steps allows precise ion track assignment to cells and correlation with cellular readouts. For complete details on the use and execution of this protocol, please refer to Niklas et al. (2022).

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